ISSN:
0749-1581
Keywords:
13C NMR
;
DNA-protein complex
;
Isotopic labeling
;
HMQC-NOESY
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Carbon-13 and proton resonance assignments for a DNA fragment complexed with its regulatory protein were obtain with the use of a selectively 13C-labelled oligonucleotide. Data obtained on the Lac operator-Lac repressor complex from the HMQC-NOESY maps of the free and complexed Lac operator demonstrate that only minor conformational modifications are induced on complexation.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260321007
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