ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Thin layer chromatography
;
Ammonium tungstophosphate
;
Aminoacids
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The chromatographic behaviour of aminoacids on columns of silica coated with different percentages of ammonium tungstophosphate at several buffer phosphate concentrations is reported. The stationary phase shows a high selectivity towards aminobutyric acids and methyl derivatives of tryptophan due to the distance between the carboxylic group and the −NH 3 + group involved in the cation-exchange process and/or to the steric hindrance by a methyl substituent. A relationship between HPLC and HPTLC data has been verified and applied to the prediction of the separation of six aminoacids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02311405
Permalink