ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract : Cyclic nucleotide-gated channels have been proposed tomediate the electrical response to light in the ventral photoreceptor cells ofthe horseshoe crab, Limulus polyphemus. However, a cyclicnucleotide-gated channel has not been identified from Limulus. Wehave cloned a putative full-length cyclic nucleotide-gated channel cDNA byscreening cDNA libraries constructed from Limulus brain using a probedeveloped from Limulus ventral eye nerves. The putative full-lengthcDNA was derived from two overlapping partial cDNA clones. The open readingframe encodes 905 amino acids ; the sequence shows 44% identity to that of theα subunit of the bovine rod cyclic GMP-gated channel over the regioncontaining the transmembrane domains and the cyclic nucleotide binding domain.This Limulus channel has a novel C-terminal region of ~200 aminoacids, containing three putative Src homology domain 3 binding motifsand a putative coiled-coil domain. The possibility that this cloned channel isthe same as that detected previously in excised patches from thephotoreceptive membrane of Limulus ventral photoreceptors is discussed in terms of its sequence and its expression in the ventral eye nerves.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1471-4159.1999.0720461.x
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