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  • 1
    Electronic Resource
    Electronic Resource
    Cloning of a Ca2+/calmodulin-dependent protein kinase gene from the filamentous fungus Arthrobotrys dactyloides (2002)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 212 (2002), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A Ca2+/calmodulin-dependent protein kinase (CaMK) gene was cloned and characterized from Arthrobotrys dactyloides, a nematode-trapping fungus. The resulting 373-amino-acid protein, FCaMK, has significant homology to mammalian CaMKs. FCaMK contains a serine/threonine kinase domain followed by a calmodulin-binding domain. The activation loop in FCaMK (amino acids 184–199) contains a phosphorylation site at threonine-188, which could be the target of a kinase activator. Truncated FCaMK mutants revealed that amino acids 296–324 are essential for calmodulin binding. An oligopeptide designed from residues 297–324 formed a stable peptide–calmodulin complex of 1:1 stoichiometry. Southern blot analysis detected a single copy of the fcamk gene, suggesting that FCaMK plays an important role in Ca2+/calmodulin signaling in A. dactyloides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2002.tb11237.x
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  • 2
    Electronic Resource
    Electronic Resource
    Biological clock in the prokaryote Synechococcus RF-1 (1994)
    Springer
    Planta 193 (1994), S. 131-136 
    Details
    ISSN: 1432-2048
    Keywords: Biological clock ; Circadian rhythm ; Leucine uptake ; Nitrogenase activity ; Polypeptide synthesis ; Prokaryote ; Synechococcus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The prokaryote Synechococcus RF-1 exhibits circadian rhythms of nitrogen fixation and leucine uptake after being entrained in a diurnal light/dark regimen. The rhythms can also be established by small temperature changes, in either a raised- or a lowered-temperature cycle, within the growth-permissive range. At the biochemical level, the circadian oscillator in Synechococcus RF-1 controls, in addition to the nitrogenase, the synthesis rate at which more than ten polypeptides are synthesized. At the physiological and the biochemical levels, the overt rhythms have fixed phase relationships, though the phase is dependant on the particular induction stimuli. A “clock” consisting of a molecular complex which can respond differentially to different induction conditions is hypothesized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00191616
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  • 3
    Electronic Resource
    Electronic Resource
    Forced calcium entry and polarized growth of Funaria spores (1979)
    Springer
    Planta 144 (1979), S. 401-406 
    Details
    ISSN: 1432-2048
    Keywords: A23187 gradients ; Calcium ; Germination (moss spores) ; Electric fields-Funaria ; Ionophore gradient ; Moss spores-Polarization ; Spores (moss)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have used both steady electric fields, and gradients of the divalent ionophore, A23187, to control the point at which rhizoids emerge from spores of the common moss Funaria hygrometrica. The spores were grown in a medium containing calcium nitrate as the only major salt. Spores tend to form rhizoids towards the positive electrode, with a half maximal response to a difference of 4–8 mV across each cell. They also tend to form rhizoids towards the end of higher ionophore concentration in response to A23187 gradients. Both of these responses are the same at pH 5.5 and 8.0. Our tentative explanation is that Funaria spores tend to form rhizoids where most calcium enters. However, the point of chloronema emergence is scarcely affected by steady fields of up to 45 mV/cell. Moreover, when steady fields are applied across already developed rhizoids or chloronemata, their subsequent growth is directed towards the negative electrode in both cases, with rhizoids giving a 50% response at only 3—5 mV/cell, and chloronemata being less responsive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380114
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of membrane potential on calcium fluxes of Pelvetia eggs (1978)
    Springer
    Planta 140 (1978), S. 63-67 
    Details
    ISSN: 1432-2048
    Keywords: Calcium fluxes ; Fucoid eggs ; Ion fluxes ; Membrane potential ; Pelvetia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 45Ca2+ fluxes across the plasma membrane of zygotes of the fucoid alga, Pelvetia fastagiata (J. Ag.) De Toni, were studied in artificial sea waters of various potassium concentrations. Except for two cases, hyperpolarization of the cell membrane (with low [K+]) increases, and depolarization (with high [K+]) decreases the influx of Ca2+ over the range of [K+] studied (1–100 mM). The fractional increases of influx during hyperpolarization are close to the fractional increases in membrane potential but the decreases during depolarization are much smaller than those in membrane potential. In two anomalous cases, the influxes of 45Ca2+ at a potassium concentration of 30 mM were about 20% higher than the control value instead of being 10% lower. The effluxes of 45Ca2+ are increased by both hyperpolarization and by depolarization. On balance (and excepting the two anomalous cases) the net result of hyperpolarization should be to increase and that of depolarization to decrease intracellular [Ca2+].
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389381
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  • 5
    Electronic Resource
    Electronic Resource
    Calcium requirement in nitrogen fixation in the cyanobacterium Synechococcus RF-1 (1988)
    Springer
    Planta 173 (1988), S. 253-256 
    Details
    ISSN: 1432-2048
    Keywords: Calcium and N2 fixation ; Cyanobacteria ; Nitrogen fixation and Ca2+ ; Rhythm, circadian (in N2 fixation) ; Synechococcus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Under diurnal 16/8-h light-dark cycles, ethyleneglycol-bis-(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) at 1 mM completely blocked the appearance of rhythmic N2-fixing activity in Synechococcus RF-1. Ca2+ at 2 mM, when supplied either together with or several hours after the EGTA application, restored the nitrogenase activity, whereas, when Ca2+ was supplied several hours later, the peak of nitrogenase activity was shifted from the dark to the light period in which the activity is normally suppressed. Sr2+ also reversed the inhibition by EGTA, but only partially. When O2 in the gas phase above the culture was below 1%, the inhibition of nitrogenase activity by EGTA was reduced to less than 20% of the control value without EGTA. Thus Ca2+ appears to be required by the cell to protect its nitrogenase from inactivation by O2. In media without EGTA, a close correlation between nitrogenase activity and concentrations of Ca2+ was also observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403017
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  • 6
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and plant regeneration from cultured young inflorescences of Oryza sativa L. (rice) (1985)
    Springer
    Plant cell, tissue and organ culture 3 (1985), S. 51-54 
    Details
    ISSN: 1573-5044
    Keywords: Oryza sativa ; rice ; callus ; inflorescence ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Compact calli initiated from young inflorescences of Oryza sativa L. (rice) on the Linsmaier and Skoog's (LS) medium containing 1 to 2.5mg/l of 2,4-dichlorophen-oxyacetic acid (2,4-D) were used for regeneration studies. After smooth and compact nodules appeared, these calli were transferred to the regeneration medium containing indole-3-acetic acid (IAA) and either kinetin or 6-benzylaminopurine (BAP). Somatic embryos developed in ten days and were examined by histological studies. Some of the embryos showed scutellum-like structures and a coleoptile-coleorhiza bipolar organization. Regenerated plants had the normal chromosome number of 2n=24.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041655
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