ISSN:
1573-2576
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Addition of cultured and then carefully-washed bovine pulmonary artery endothelial cells (EC) decreased (p 〈 0.05) human neutrophil elastase activity (HNE) in vitro. HNE activity was also decreased (p 〈 0.05) by addition of histone or protamine treated EC. However, addition of papain or trypsin treated EC decreased HNE activity less than addition of untreated cells suggesting that a protein rather than a difference in cell surface charge was responsible. Other observations suggest that EC anti-elastolytic activity was not due to binding of antiprotease from culture media but was dependent on EC protein synthesis. First, addition of EC grown previously in serum-free media decreased HNE activity the same (p 〈 0.05) as addition of EC cultured in media containing serum. Second, addition of EC treated beforehand with cycloheximide decreased HNE activity less than (p 〈 0.05) addition of untreated control EC. We conclude that EC most likely make and have antielastolytic activity on their surfaces and speculate that EC associated anti-elastolytic activity may modulate inflammatory, repair and other biologic processes involving neutrophil elastase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01488207
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