ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
The purpose of this study is to make use of trans10,cis12 CLA (t-CLA) that has potentialfor proliferation and differentiation to form adipocyte on the collagen-coated surface. Resultsprovided evidences of good adhesion, growth, viability, and differentiation of adipocyte oncollagen-coated surface compared with non-coated surface. Also, the results showed that mouse3T3-L1 preadipocyte can be successfully and reproducibly cultured on the collagen-coated surface,and the adipocyte precursor cells placed on the collagen-coated surface are able to undergo fullmaturation into adipocytes in the control cells. Moreover, glycerol-3-phosphate dehydrogenase(GPDH) activity in 3T3-L1 preadipocyte cultured on collagen-coated surface with t-CLA washigher than that on polystyrene (PS) surface due to higher cell adhesion and cell viability. Theseresults suggest that collagen coating may provide a promising approach to develop a new adipocytereplacement strategy using CLA
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.261.pdf
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