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  • 1
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Science Ltd
    European journal of neuroscience 10 (1998), S. 0 
    ISSN: 1460-9568
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: It is well known that noradrenergic locus coeruleus (LC) neurons decrease their activity during slow wave sleep (SWS) and are virtually quiescent during paradoxical sleep (PS). It has been proposed that a GABAergic input could be directly responsible for this sleep-dependent neuronal inactivation. To test this hypothesis, we used a new method combining polygraphic recordings, microiontophoresis and single-unit extracellular recordings in unanaesthetized head-restrained rats. We found that iontophoretic application of bicuculline, a specific GABAA-receptor antagonist, during PS and SWS restore a tonic firing in the LC noradrenergic neurons. We further observed that the application of bicuculline during wakefulness (W) induced an increase of the discharge rate. Of particular importance for the interpretation of these results, using the microdialysis technique, Nitz and Siegel (Neuroscience 1997; 78: 795) recently found an increase of the GABA release in the cat LC during SWS and PS as compared with waking values. Based on these and our results, we therefore propose that during W, the LC cells are under a GABAergic inhibitory tone which progressively increases at the entrance and during SWS and PS and is responsible for the inactivation of these neurons during these states.
    Materialart: Digitale Medien
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  • 2
    ISSN: 1460-9568
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Midbrain dopamine neurons in vivo discharge in a single-spike firing pattern or in a burst-firing pattern. Such activity in vivo strikingly contrasts with the pacemaker activity of the same dopamine neurons recorded in vitro. We have recently shown that burst activity in vivo of midbrain dopamine neurons is due to the local activation of excitatory amino acid receptors, as microapplication of the broad-spectrum antagonist of excitatory amino acids, kynurenic acid, strongly regularized the spontaneous firing pattern of these dopamine neurons. In the present study, we investigated which subtypes of excitatory amino acid receptors are involved in the burst-firing of midbrain dopamine neurons in chloral hydrate-anaesthetized rats, Iontophoretic or pressure microejections of 6-cyano,7-nitroquinoxaline-2,3-dione (CNQX), a non-N-methyl-d-aspartate (NMDA) receptor antagonist, did not alter the spontaneous burst firing of dopamine neurons (n= 36). In contrast, similar ejections of (±)2-amino,5-phos-phonopentanoic acid (AP-5), a specific antagonist at NMDA receptors, markedly regularized the firing pattern by reducing the occurrence of bursts (n= 52). In addition, iontophoretic ejections of NMDA, but not kainate or quisqualate, elicited a discharge of these dopamine neurons in bursts (n= 20, 12 and 14, respectively). These data suggest that burst-firing of midbrain dopamine neurons in vivo results from the tonic activation of NMDA receptors by endogenous excitatory amino acids. In view of the critical dependency of catecholamine release on the discharge pattern of source neurons, excitatory amino acid inputs to midbrain dopamine neurons may constitute a major physiological substrate in the control of the dopamine level in target areas.
    Materialart: Digitale Medien
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  • 3
    ISSN: 1460-9568
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Microiontophoretic application of selective agonists for the three major excitatory amino acid receptors, N-methyl-d-aspartate (NMDA), quisqualate and kainate, increased the discharge rate of noradrenergic locus coeruleus (LC) neurons in vivo. NMDA activation was selectively attenuated by iontophoretic application of 2-amino-5-phosphonopentanoate (AP5), an antagonist at NMDA receptors, whereas kainate- and quisqualate-evoked responses were attenuated by both NMDA and non-NMDA antagonists iontophoresis. NMDA- and quisqualate-evoked responses were significantly decreased by co-iontophoresis of serotonin (5-HT). When the NMDA receptor-mediated component of the response to kainate was blocked with AP5 iontophoresis, 5-HT increased the response of LC neurons to kainate. These results revealed that 5-HT differentially modulates the responsiveness of LC neurons to excitatory amino acids, depending on the receptor subtypes responsible for the neuronal activation.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    ISSN: 1435-1463
    Schlagwort(e): Norepinephrine ; electrophysiology ; α-adrenergic receptors ; cerebellum ; hippocampus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Previous pharmacological characterization of central noradrenergic receptors has been interpreted as favoring beta-type receptors on cerebellar Purkinje neurons and hippocampal pyramidal neurons. However, the recent development of additional noradrenergic antagonists suitable for single neuron analysis, prompted an initial re-evaluation of alpha and beta adrenergic receptors in these two cell populations. In contrast with earlier data based on local antagonism of iontophoretric or synaptically released norepinephrine (NE), we now find that systemic phenoxybenzamine, the alpha antagonist, was effective in blocking responses to NE in cerebellum and hippocampus, whereas systemic beta antagonists metoprolol, ICI 181.551, or proprandol did not interfere with local NE responses at systemic doses that altered spontaneous discharge rates. These preliminary data suggest that a more complete re-evaluation of the nature of central noradrenergic response mechanisms may be warranted.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Pflügers Archiv 365 (1976), S. 183-189 
    ISSN: 1432-2013
    Schlagwort(e): Temperature regulation ; Circadian rhythms ; Ambiant temperature ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Résumé L'enregistrement chronique des températures cérébelleuses et sous-cutanées par thermistance a été réalisé chez le rat soumis à différentes températures ambiantes et à une alternance lumière-obscurité de 12 h (lumière de 7 à 19 h). La période du rythme des températures cérébelleuses et sous-cutanées est de 24 h avec acrophase nocturne (1 h) et amplitude de 0,75° C. L'amplitude et l'acrophase ne sont pas modifiées au cours des expositions à différentes températures ambiantes (20-25-30-34-35° C). Chaque augmentation d'ambiance se traduit par une élévation du niveau moyen de la température interne. Cette augmentation, peu perceptible entre 25 et 30° C, atteint 0,5° C entre 20 et 25° C et 1° C entre 30 et 34° C. Elle se maintient tout au long des 10 jours d'observation. Ces résultats soulèvent au moins 3 questions: le degré de liaison entre les rythmes d'activité (éveil) et de température, la labilité de la température interne qui varie avec l'ambiance alors que l'amplitude du rythme circadien reste constante, l'absence de diminution de la température interne après plusieurs jours en ambiance chaude alors qu'activité et métabolisme sont diminués dès le 2ème jour d'exposition.
    Notizen: Summary Chronic recording of cerebellar and subcutaneous temperatures were carried out in rats maintained at different ambient temperatures in a 12 h light-dark cycle (light from 7 to 19 h). Cerebellar and subcutaneous temperatures followed a rhythm with a period of 24 h with acrophase at 1 h and an amplitude of 0.75° C. The amplitude and acrophase were not altered by modification of the ambient temperature (20-25-30-34 or 35° C), but each elevation of ambient temperature produced a rise in the mean internal temperature of the rat. This rise, hardly perceptible at ambient temperatures, of 25 and 30° C, reaches 0.5° C between 20 and 25° C and 1° C between 30 and 34° C. This elevation of temperature was maintained for the duration of the 10 days of observation. These results pose at least three questions: the degree of liaison between the rhythms of activity (waking) and temperature; the lability of the mean internal temperature, which alter with ambient temperature while the amplitude of the circadian rhythm is unaltered and the absence of reduction of mean internal temperature several days exposure to a raised ambient temperature, even when activity and metabolism are reduced by the second day of exposure.
    Materialart: Digitale Medien
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