ISSN:
1617-4623
Keywords:
Recombination
;
Plasmid
;
Streptomyces
;
Escherichia coli
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary An Escherichia coli-Streptomyces shuttle plasmid pIF132 containing two direct mel repeats was constructed. While pIF132 replicated relatively stably in E. coli (Rec+ or recA), its structure was unstable in S. lividans: recombination between the mel repeats resulted in a smaller plasmid, pIF138. Furthermore, pIF132 formed oligomers extensively in E. coli but not in S. lividans.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00329851
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