ISSN:
1573-6784
Keywords:
indirubin
;
two-phase suspension culture
;
Polygonum tinctorium
;
dimethylpolysiloxane
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Dimethylpolysiloxane (DMPS), used as a second phase in a suspension culture of Polygonum tinctorium, increased indirubin production by up to 259% and 16% in shake flask and air-lift bioreactors, respectively. In the shake flask culture, indirubin production increased by up to 248 mg/l using 90% v/v DMPS (volume of DMPS/volume of medium). In an air-lift bioreactor culture grown at a perfusion rate of 0.05 day−1 the indirubin concentration reached a maximum of 130 mg/l using 50% (v/v) DMPS. For the same DMPS content, the lower indirubin production in an air-lift bioreactor was probably due to the mass transfer limitation of indirubin between the two phases. © Rapid Science Ltd. 1998
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008800331295
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