ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract— Studies were made on isolated ganglia of the locust in a modified O2 electrode. The basic requirements of a suitable incubation medium were determined and found to include saturation of the medium with 100% O2, and 10mm-glucose as energy source. The effects of incubation in vitro were determined for a number of biochemical characteristics including O2 uptake and the concentrations of Arg P, ATP, ADP, glycogen, glucose, lactate and pyruvate. Action potentials were recorded from a major nerve both in vivo and in vitro. On the basis of these biochemical and physiological studies, it was concluded that the tissue functions well in vitro. Some comparisons were made between the metabolic characteristics found in the locust nervous system, and those of mammalian tissue. The locust ganglia were found to have a rate of O2 uptake much higher than comparable mammalian tissue, and to contain a considerable reserve of glycogen. The low ratio of lactate/pyruvate suggests a more aerobic metabolism than is the case in mammalian brain.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1978.tb12441.x
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