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  • 1
    Electronic Resource
    Electronic Resource
    Erythrosin B as an effective inhibitor of electrogenic H+ extrusion (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 9 (1986), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract In 24-h-genninaled radish seedlings erythrosin B (EB), an effective inhibitor of microsomal as well as of partially purified vanadate-sensitive ATPase markedly inhibited the basal and the FC-stimulated proton extrusion, and induced a rapid depolarization of FC-hyperpolarized trans-membrane electric potential (PD) without causing any significant change of ATP level. The effects of EB on H+ extrusion were partially additive with those of vanadatc, another inhibitor of plasma membrane H+-ATPase. These effects are interpreted as due to a direct inhibition by EB on plasma membrane H+-ATPase involved in H+ electrogenic transport in the higher plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1986.tb01626.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Mycochromone decreases ATP-driven proton translocation in plant plasma membrane- and tonoplast-enriched vesicles (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 10 (1987), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Mycochromone, a metabolite produced by Mycosphaerella rosigena, inhibits the ATP-dependent proton translocation and the ATP-generated electrical potential in pea stem tonoplast-enriched vesicles, without affecting the H+/K+ exchange induced by nigericin or an artificially imposed proton gradient. The inhibition is dependent on the time of pre-incubation and mycochromone concentration. In addition, mycochromone inhibits the ATP-dependent proton translocation in radish plasma membrane-enriched vesicles, though it does not alter ATPase activity (evaluated by hydrolysis of ATP) in either type of plant vesicle. Mycochromone seems to act on the H+ channels for proton translocation of the H+-pumping ATPase localized on plasmalemma and tonoplast, without affecting the catalytic site of ATP hydrolysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/1365-3040.ep11603612
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of canavanine on IAA- and fusicoccin-stimulated cell enlargement, proton extrusion and potassium uptake in maize coleoptiles (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 48 (1980), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In maize coleoptiles (Zea mays F1 XL 640A, cv. Dekalb) canavanine and cycloheximide strongly and simultaneously inhibit cell elongation, H+ extrusion and K+ uptake, induced by IAA. They inhibit also, although to a much lesser degree, the same phenomena induced by fusicoccin. Cycloheximide severely depresses the incorporation of leucine into proteins, while canavanine leaves leucine incorporation almost unchanged. The data confirm that elongation, H+ extrusion and K+ uptake can be regarded as correlated processes; they also support the view that normal protein synthesis is essential for IAA-induced growth, while this requirement is only partial in growth stimulated by fusicoccin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1980.tb03248.x
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  • 4
    Electronic Resource
    Electronic Resource
    Regulation of isocitrate lyase level in yeast growing on external carbon sources or on lipid reserves (1969)
    Springer
    Cellular and molecular life sciences 25 (1969), S. 131-132 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Riassunto Quando il lievitoRhodotorula gracilis cresce sulle proprie riserve lipidiche si registra un aumento dell'enzima isocitrato liasi, fino ad un livello paragonabile come ordine di grandezza a quello raggiunto quando il lievito cresce su etanolo. Il glucosio reprime vigorosamente la sintesi dell'enzima, anche in presenza di etanolo. Dai dati finora noti un effetto induttivo dell'etanolo sembra da escludere, e la regolazione del livello di isocitrato liasi appare basata su meccanismi di repressione da cataboliti.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01899079
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Biochemical and morphological aspects of zinc deficiency in rhodotorula gracilis (1972)
    Springer
    Archives of microbiology 85 (1972), S. 267-279 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cells of Rhodotorula gracilis cultured in a liquid medium containing Zn only as impurity stop growing at a density of about 5·107 cells/ml. The addition of Zn during the prestationary or the stationary phase of growth reestablishes the growth rate, thus showing that Zn++ is a limiting factor for growth. An analysis of the changes of the fine structure and of RNA, DNA and protein levels induced by the addition of Zn to Zn-deficient cultures indicates that the most notable features of Zn deficiency are: a) the decrease of net RNA synthesis, and consequently of protein synthesis; b) the appearance of several large vacuole-like structures containing degraded cytoplasmic components, membranous whorls and amorphous material; a decrease in the number of mitochondria and in the organization of cristae. The net synthesis of DNA appears to be much less affected, and lipid synthesis is somewhat stimulated in the Zn-deficient cultures. No important effect of Zn-deficiency was observed on either oxygen uptake or intracellular amino acid level. These results are interpreted as indicating that Zn++ is an essential element for this organism, and that the area of RNA metabolism and protein synthesis is the one primarily affected by Zn++ deficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00549265
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Oxygen pressure, fatty acid composition and ergosterol level in Rhodotorula gracilis (1975)
    Springer
    Archives of microbiology 105 (1975), S. 17-20 
    Details
    ISSN: 1432-072X
    Keywords: Rhodotorula gracilis ; pO2 ; Fatty Acid ; Ergosterol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of Rhodotorula gracilis grown for 6 hrs at 2 mm Hg O2 pressure when compared with cells grown for 6 hrs at 140 mm Hg, show: a) a large decrease in the level of ergosterol, b) a significant increase in the level of oleic acid and a decrease in the levels of linoleic and linolenic acids, both in the fatty acid fraction of the phospholipids and in the free fatty acids and neutral fat fractions. The results suggest that the dehydrogenation of oleic acid to linoleic acid is preferentially inhibited at low O2 pressure. The possibility is considered that these changes of lipid metabolism might be causally related with decrease of the growth rate observed at low O2 pressure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447106
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    Paper (German National Licenses)
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