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  • 1
    Electronic Resource
    Electronic Resource
    Preparation of 67Ga-labelled human IgG and its Fab fragments using desferoxamine as chelating agent (1985)
    Springer
    European journal of nuclear medicine 11 (1985), S. 240-245 
    Details
    ISSN: 1619-7089
    Keywords: Desferoxamine ; Gallium ; Human gamma globulin ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Human IgG and its Fab fragments were chosen as a model system for studying the desferoxamine (DF) coupling reaction using glutaraldelyde or carbodiimide at various concentrations. The labelling of the IgG-DF conjugates with 67Ga proved to be highly efficient and reproductible. The labelling efficiency in relation to storage time of the conjugate was analysed over a 1-month period by Sephadex-G-50 chromatography. Gel-filtration analysis on Sephacryl S-300 showed that, following the coupling procedure, a high proportion (more than 80%) of the conjugate remained monomeric. Immunoelectrophoresis on agar plates demonstrated that the antibody immunoreactivity was preserved. The biodistribution in mice of the 67Ga-DF-IgG conjugates was comparable to that of the conventional radiopharmaceutical, 125I-HSA. Conditions were established for the coupling of DF to two rat IgG2b monoclonal antibodies M10/76 and 11/160, that are specific for the Hooded rat sarcomata MC 24 and HSN respectively; the immunoreactivity of these conjugates was tested by a cellbinding assay. The data indicate that monoclonal-antibody/DF conjugates prepared with the bifunctional reagent glutaraldelyde maintain their capacity for binding to their tumour-associated antigens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279077
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    99mTc-HMPAO as a tumour blood flow agent (1987)
    Springer
    European journal of nuclear medicine 13 (1987), S. 90-94 
    Details
    ISSN: 1619-7089
    Keywords: Tumours ; Blood flow ; Propranolol ; Anaesthesia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The cerebral blood flow agent, 99mTc-HMPAO (‘CeretecTM’) has been investigated to see if it can be used to estimate tumour blood flow. Its distribution in Balb/c mice bearing either a subcutaneously implanted sarcoma or a plasmacytoma has been shown to be similar to that of 86RbCl. The changes in peripheral blood flow caused by the beta-blocker, propranolol, and by Nembutal anaesthesia, are manifested equally by 99mTc-HMPAO and 86Rb. We conclude, therefore, that HMPAO may be useful in estimating tumour perfusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00256023
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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