ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract: In PC12 cells, preincubated with [3H]inositol, nerve growth factor (NGF) stimulated an ∼ 100% increase in the levels of [3H]inositol 1,3,4-trisphosphate {[3H]-Ins(1,3,4)P3}, [3H]inositol 1,4,5-trisphosphate {[3H]lns(1,4,5)P3}, and [3H]inositol 1,3,4,5-tetrakisphosphate {[3H]-Ins(1,3,4,5)P4} as early as 5–15 s after addition of NGF. This NGF-mediated response was apparent only when the cells had been cultured in the absence of fetal bovine serum (FBS). PC12 cells cultured in FBS-containing medium did not display NGF-mediated increases in [3H]-Ins(1,3,4)P3, [3H]-Ins(1,4,5)P3, and [3H]-Ins(1,3,4,5)P4 levels. Using cells cultured in the absence of FBS, epidermal growth factor (EGF) and fibroblast growth factor also stimulated production of [3H]lns(1,3,4)P3, [3H]-Ins(1,4,5)P3, and [3H]lns(1,3,4,5)P4. Lavendustin A, a tyrosine kinase inhibitor, inhibited both the EGF-and NGF-stimulated increases in the levels of these tritiated inositol phosphates. These results suggest that NGF stimulates the production of lns(1,3,4)P3, lns(1,4,5)P3, and lns(1,3,4,5)P4 and that this response is dependent on tyrosine kinase activity. Furthermore, although the production of lns(1,3,4)P3, lns(1,4,5)P3, and lns(1,3,4,5)P4 may be a common response to factors stimulating neuronal differentiation, it is not sufficient for stimulation of neuronal differentiation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1993.tb03617.x
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