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Regional Distribution of the GABAA/Benzodiazepine Receptor (α Subunit) mRNA in Rat Brain (1988)

Montpied, Pascale ; Martin, Brian M. ; Cottingham, Sandra L. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 51 (1988), S. 0

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ISSN: 1471-4159

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Abstract: A human cDNA clone containing the 5’coding region of the GABAA/benzodiazepine receptor α subunit was used to quantify and visualize receptor mRNA in various regions of the rat brain. Using a [32P]CTP-labelled antisense RNA probe (860 bases) prepared from the α subunit cDNA, multiple mRNA species were detected in Northern blots using total and poly A rat brain RNA. In all brain regions, mRNAs of 4.4 and 4.8 kb were observed, and an additional mRNA of 3.0 kb was detected in the cerebellum and hippocampus. The level of GABAA/benzodiazepine receptor mRNA was highest in the cerebellum followed by the thalamus = frontal cortex = hippocampus = parietal cortex = hypothalamus ≫ pons = striatum = medulla. In situ hybridization revealed high levels of α subunit mRNA in cerebellar gray matter, olfactory bulb, thalamus, hippocampus/dentate gyrus, and the arcuate nucleus of the hypothalamus. These data suggest the presence of multiple GABAA/benzodiazepine receptor α subunit mRNAs in rat brain and demonstrate the feasibility of studying the expression of genes encoding the GABAA/benzodiazepine receptor after pharmacological and/or environmental manipulation.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1471-4159.1988.tb01137.x

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Tyrosine hydroxylase and cholecystokinin mRNA levels in the substantia nigra, ventral tegmental area, and locus ceruleus are unaffected by acute and chronic haloperidol administration (1990)

Cottingham, Sandra L. ; Pickar, David ; Shimotake, Thomas K. ; [et al.]

Springer

Cellular and molecular neurobiology 10 (1990), S. 41-50

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ISSN: 1573-6830

Keywords: tyrosine hydroxylase ; cholecystokinin ; mRNA ; in situ hybridization ; antipsychotics ; haloperidol ; reserpine ; substantia nigra ; ventral tegmental area ; locus ceruleus ; catecholamine ; dopamine

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary 1. The studies described herein were designed to test the hypothesis that a neuroleptic, haloperidol, may alter the level of expression of the tyrosine hydroxylase and cholecystokinin genes in discrete brain regions. 2. In situ hybridization was employed to quantitate changes in concentration of mRNA for tyrosine hydroxylase and cholecystokinin in the ventral tegmental area, substantia nigra, and locus ceruleus after acute or chronic treatment with haloperidol or vehicle. 3. Haloperidol had no effect on the level of tyrosine hydroxylase or cholecystokinin mRNAs, in the ventral tegmentum, substantia nigra, or locus ceruleus, at either 3 or 19 days of drug administration. 4. These data suggest that haloperidol administration does not alter the level of tyrosine hydroxylase or cholecystokinin mRNAs in midbrain dopamine neurons of the rat.