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  • 1
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 51 (1995), S. 1834-1836 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    European Journal of Medicinal Chemistry 29 (1994), S. 471-477 
    ISSN: 0223-5234
    Keywords: aldose reductase inhibitor ; antioxidant ; pyridazinone
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A precocious line ofEimeria intestinalis was obtained by selection for early development of oocysts in rabbits and after six consecutive passages in animals. This line (EiP) was derived from a wild strain (EiO) isolated in 1975 from the caecal content of a rabbit with coccidiosis. The prepatent period of the EiP strain was reduced from 215 h to〈144 h, the result being that the oocyst sporulation time was the same for both lines. The excreted and unsporulated oocysts had exactly the same shape, but microscopical examination of the sporulated oocysts showed a marked difference between EiP and EiO strains. A huge refractile globule was located in each of two sporocysts of the precocious line, whereas no refractile globule was seen in the other two. The EiP line had a reproductive potential much lower (1000 times) than that of its parent strain EiO and, as judged by the weight gain, mortality and lesions that also occurred in the jejunum and above all in the ileum, its pathogenicity was substantially reduced.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 79 (1993), S. 593-598 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The invasive phase ofEimeria coecicola was studied during the first 80 h postinoculation (p.i.). Using a method that synchronized the life cycle, sporozoites were observed in the duodenum and the jejunum until 32 h p.i. They were seen first in the villous epithelial cells or in host cells resembling intraepithelial lymphocytes (IEL). Later they were observed in IEL in the lamina propria. After 48 h p.i., no coccidian stage was identifiable in the mucosa of the small intestine but sporozoites appeared in the lymphoid cells of lymphatic follicles of the gut-associated lymphoid tissue (vermiform appendix, sacculus rotundus, and Peyer's patches). The first merogony was observed 64 h p.i. in these lymphoid cells and in membranous epithelial cells (M-cells) but was never seen in the epithelium itself. Morphologically there were two types of meronts, depending on the host cell type, but in both cases the merozoites contained a refractile body and resembled sporozoites. The first meronts of the second generation were observed 80 h p.i. in the villous epithelial cells of the domes of the follicles of the gut-associated lymphoid tissue, where the further development of thisEimeria takes place. This pattern of invasion strongly suggests that sporozoites take an exclusively extraintestinal route to reach the target cells. Moreover, to our knowledge this is the first description of an eimerian merogony that does not take place in epithelial cells.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The pathogenicity and immunogenicity ofEimeria intestinalis was evaluated in SPF rabbits. The antimals were given immunizing doses of 6, 6×102, 6×103, and 6×104 sporulated oocysts and were challenged with 3×103 oocysts. The criteria analysed were the daily weight gain and the occyst output. This study showed thatE. intestinalis had strong immunogenicity, as the inoculation of 6 oocysts was sufficient to minimize the clinical expression of the disease following the challenge and to reduce the oocyst output by about 60%. The immunity towards the excretion of oocysts and the illness was absolute in animals inoculated with 600 or more oocysts. Moreover, this protection seemed to be efficient at least 8 weeks after the challenge. The present results also confirm the pathogenicity ofE. intestinalis, although the occurrence of diarrhoea may be irregular, and emphasize the fact that the capacity of thisEimeria for multiplication is not a criterion for clinical diagnosis of the disease.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 80 (1994), S. 48-52 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A precocious line ofEimeria media was obtained by selection for early development of oocysts in rabbits. The prepatent period was reduced from 108 to 72 h. The precocious line was less pathogenic than the original strain, and its multiplication rate was lower. Rabbits given oocysts of the precocious line were totally immune to challenge with the original strain as assessed by change in weight gain but were partially protected as assessed by oocyst output. Selection for precocious development was accompanied by morphological changes in the sporulated oocysts; each sporocyst contained only one large refractile body instead of the two smaller bodies seen in the original strain.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 82 (1996), S. 347-351 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Coccidia-free rabbits were inoculated with different doses of a pure strain of Eimeria coecicola and samples of gut were taken at 80, 96, 112, 128, 144, and 160 h postinoculation. The use of a very low infective dose (2–20 oocysts) was sufficient to study the last merogony. The number of merozoites in meronts increased when the infective dose decreased. Only the first merogony of this coccidium in lymphocytes or M-cells of gut-associated lymphoid tissue (GALT) has previously been described. Three other generations of meronts are described herein. All these endogenous stages were observed in the epithelium of the vermiform appendix, sacculus rotundus, and Peyer’s patches, especially at the bases of the domes. However, in heavily infected tissues the gamonts were seen throughout the epithelium of the GALT. The third- and fourth-generation meronts were of two types. As in other eimerian species of the rabbit, type A meronts produced thick polynucleated merozoites, whereas type B meronts gave rise to large numbers of thin merozoites with one nucleus. Microgamonts were polynucleated and less numerous than macrogamonts. Type A meronts were also polynucleated and less numerous at the end of the merogony. Therefore, types A and B could correspond to a sexual phenotype differentiation occuring during the two asexual phases of multiplication.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 85 (1999), S. 850-854 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The endogenous development of the rabbit coccidium Eimeria vejdovskyi was studied in SPF rabbits using light and electron microscopy. All endogenous stages were seen in the ileal epithelium. There were two types of meronts in each of five asexual generations. Type A produced fat, polynucleate merozoites while type B meronts had slender uninucleate merozoites. First- second- and third-generation meronts were in the crypts. The first meronts were at the bottom of the crypts, very often in the Paneth cells. The meronts of the fourth generation were in the middle of the villi; the fifth-generation meronts were recorded in the middle and at the top of the villi. The prepatent period was 10 days.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 59 (1979), S. 227-234 
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Summary Eimeria perforans was isolated in pure strain by utilizing specific biological characters and specific-pathogen free rabbits. The purity of this strain was demonstrated by measurements of oocysts, derived from a line, started by infection from a single oocyst. The parasite, when inoculated in large numbers, causes a mild disease response for only about two days.
    Notes: Zusammenfassung Die sogenannten kleinen Coccidien sind morphologisch schwierig zu unterscheiden. Um einen reinen Stamm von Coccidien zu erhalten, müssen absolut coccidienfreie Kaninchen zur Verfügung stehen. Solche werden am INRA in Tours durch vielseitige Einrichtungen und Maßnahmen gehalten. E. perforans wurde charakterisiert durch seine Zykluslänge von 5 Tagen, einer Sporulationszeit von 21 h bei 26°C und den typischen morphologischen Kriterien der sporulierten Oocysten. Von einem Stamm, der diesen Erforderungen entsprach, wurden nach einer statistischen Verteilung der Länge und Breite der Oocysten aus dem mittleren Bereich 12 Oocysten einzeln isoliert und je in einem SPF Kaninchen vermehrt. Der so neu gewonnene Stamm, dessen Größenverteilung dem Mutterstamm am meisten entsprach, wurde als rein bezeichnet. Zur Prüfung der Pathogenität wurden 3 verschiedene Mengen verabreicht. Es traten keine Todesfälle auf, bei hohen Mengen zeigte sich der Caecuminhalt 2 Tage nach Inokulation leicht verflüssigt. Alle Tiere (alle Mengen) zeigten zwischen dem 2. und 4. Tag kleinere Gewichtszunahmen als die Kontrolltiere. Parallel dazu war auch die Futteraufnahme entsprechend vermindert. Mit diesen Befunden wirdE. perforans als praktisch apathogen bezichnet.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 6 Wochen alte männliche SPF Kaninchen wurden mit einem reinen Stamm (von einer Oocyste aus gewonnen) vonE. perforans infiziert. In Zeitabständen von 12 h wurden während einer Zyklusdauer von 5 Tagen den getöteten Kaninchen mehrere Darmstücke entnommen, die anschließend licht- und elektronenmikroskopisch untersucht wurden. Durch genaue Identifizierung der einzelnen Entwicklungsstadien in den Dünndarmepithelzellen zu jedem Zeitpunkt konnte der endogene Zyklus festgelegt werden: Von 12–36 h treten Sporozoiten auf, von 24 h an finden sich Schizonten, die bei 36–48 h sehr häufig sind und deshalb der ersten Generation zugeteilt werden. Mit 84 h tritt der Höhepunkt der zweiten Schizontengeneration auf. Anschließend, von 96–108 h, erfolgt die Gametogonie mit Ausbildung der Mikro-und Makrogametocyten. Sowohl während der ersten als auch der zweiten Schizontengeneration konnten 2 Typen beobachtet werden: a) der eine mit vielen kleinen uninukleären Merozoiten, die an der Oberfläche gebildet werden. b) der andere mit wenigen, plumpen, großen multinukleären Merozoiten, die im Innern gebildet werden. Anhand von quantitativen und morphologischen Kriterien wurden die beiden Typen einer weiblich (a) und einer männlich (b) determinierten Linie zugeteilt. Dieser fürE. perforns erarbeitete Zyklus wird als Modell für Zyklen von sämtlichenEimeria-Arten aller Tiergattungen angenommen.
    Notes: Summary Specific pathogen free rabbits of certified origin were inoculated with a pure strain ofEimeria perforans that originated from a single oocyst. A sequential study of the endogenous development for the whole five day cycle was made by examining at light and electron microscopic levels samples of duodenal mucus, taken at 12 h intervals. A first schizogony was found to take place between 12 and 60 h, a second one between 72 and 84 h. Both asexual generations were represented by two types of schizonts: (1) containing 16–36 small mononucleated merozoites, produced by ectomerogony and determined as female, (2) containing 2–12 large multinucleated merozoites, produced by endomerogony and determined as male.
    Type of Medium: Electronic Resource
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