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  • 1
    Electronic Resource
    Electronic Resource
    Basophil histamine release tests in the diagnosis of allergy and asthma (2001)
    Oxford, UK : Blackwell Science, Ltd
    Clinical & experimental allergy 31 (2001), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2001.01043.x
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  • 2
    Electronic Resource
    Electronic Resource
    Basotest® and suxamethonium all_ergy (2001)
    Copenhagen : Munksgaard International Publishers
    Allergy 56 (2001), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2001.00223.x
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  • 3
    Electronic Resource
    Electronic Resource
    Immunoglobulin isotype composition of circulating and intra-articular immune complexes in patients with inflammatory joint disease (1991)
    Springer
    Rheumatology international 11 (1991), S. 169-174 
    Details
    ISSN: 1437-160X
    Keywords: Immune complexes ; Immunoglobulin isotypes ; Rheumatoid arthritis ; Flow cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The immunoglobulin (Ig) heavy chain isotype composition of intra-articular and circulating immune complexes (ICs) were determined by a Raji cell flow cytometric assay in paired serum and synovial fluid samples from 15 patients with rheumatoid arthritis (RA) and 15 patients with other articular diseases (osteoarthritis, ankylosing spondylitis, gout, psoriatic arthritis, Reiter's discase). ICs were most prevalent in synovial fluid samples of patients with RA but were infrequently detected in serum and synovial fluid samples from the non-RA patients. ICs in patients with RA were heterogeneous both in the prevalence of Ig subclasses identified and in the distribution of the respective Ig isotypes within the complexes. Furthermore, differences were observed in the Ig isotype composition of ICs in paired serum and synovial fluid samples in dicating that circulating ICs may not always arise simply by spill-over from articular sites. The possible mechanisms for IC formation in RA are discussed with reference to four patients who displayed features of extra-articular disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332556
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    CD4 subsets (CD45RA/RO) exhibit differences in proliferative responses, IL-2 and γ-interferon production during intravenous methylprednisolone treatment of multiple sclerosis (1996)
    Springer
    Journal of neurology 243 (1996), S. 475-481 
    Details
    ISSN: 1432-1459
    Keywords: Multiple sclerosis ; CD4 lymphocyte subsets ; Methylprednisolone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Phytohaemagglutinin (PHA)-induced proliferative responses, interleukin 2 (IL-2) and γ-interferon production were determined in purified CD4CD45RA and CD4CD45RO lymphocytes isolated by immunomagnetic bead separations from normal subjects and multiple sclerosis (MS) patients. Significantly higher proliferative activities were observed for CD4CD45RA cells compared with the corresponding CD4CD45RO cell population in normal subjects and MS patients. CD4CD45RA lymphocyte proliferative responses declined by 50% 3 h following a single dose (500 mg) of intravenous methylprednisolone (IVMP). At 24 h, levels were similar to those determined pre-therapy, as were the levels observed 24 h after a 5-day course (500 mg daily) of IVMP. In contrast, CD4CD45RO cells were unaffected by IVMP. In vitro incorporation of methylprednisolone (10−6 M) to cell cultures resulted in a modest reduction in proliferative activities of both CD4 subsets. In MS patients subnormal levels of IL-2 and γ-interferon were observed in PHA-stimulated cultures of CD4CD45RA and CD4CD45RO cells. Following 5 days of IVMP therapy, IL-2 and γ-interferon production was similar to that observed in CD4CD45RA and CD4CD45RO cells from normal subjects. IVMP therapy causes selective, but transient, inhibition of CD4CD45RA lymphocyte proliferative responses and enhancement of PHA-induced IL-2 and γ-interferon production by both CD4CD45RA and CD4CD45RO cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00900503
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  • 5
    Electronic Resource
    Electronic Resource
    Cytochemistry of lymphoid cells: a review of findings in the normal and leukaemic state (1984)
    Springer
    Journal of molecular histology 16 (1984), S. 1027-1050 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Conclusions The cytochemical investigation of lymphoid cells, often regarded as unrewarding, is now proving to be of value in both scientific terms and clinical practice. This new era of cytochemical research, following in the wake of pioneering work with monoclonal antibodies, is beginning to provide information on the enzymatic properties of distinctive lymphocyte subsets. Thus, cytochemical profiles of normal lymphocyte subpopulations are emerging which are providing a basis for understanding previously reported heterogeneous cytochemical reactivities. In lymphoproliferative disorders, cytochemical analyses are providing information on two fronts: (a) as a means of distinguishing differing disease types, thus aiding differential diagnosis, and (b) as a means of investigating the patterns of enzyme expression during lymphocyte development. The future will undoubtedly see further correlation of enzyme reactivities with immunological markers (particularly monoclonal antibodies) and this, in turn, will give rise to more detailed descriptions of the cytochemical properties of precisely defined lymphocyte subpopulations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01002893
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural cytochemistry of chronic T-cell leukaemias. A study with four acid hydrolases (1983)
    Springer
    Journal of molecular histology 15 (1983), S. 895-909 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural localization of four acid hydrolases (acid phosphatase, β-glucuronidase, β-glucosaminidase and α-naphthylacetate esterase) has been studied in lymphocytes from 16 patients with three types of chronic T-cell leukaemia, namely, T-prolymphocytic leukaemia (T-PLL), T-chronic lymphocytic leukaemia (T-CLL) and adult T-cell lymphoma leukaemia (ATLL). Different patterns of enzyme distribution were observed in the leukaemic T-cells from these disorders. In T-PLL, reactivity for the four acid hydrolases was confined to single or a few large granules. Gall bodies were reactive for β-glucuronidase, β-glucosaminidase and α-naphthylacetate esterase but apparently unreactive for acid phosphatase. In T-CLL, scattered small-to medium-size cytoplasmic granules and many parallel tubular arrays were strongly reactive for acid phosphatase, β-glucuronidase and β-glucosidase but showed no reactivity for α-naphthylacetate esterase. Intermediate features were observed in ATLL. The observed differences in enzyme reactivity reflect a different content of lysosomal granules in the various types of leukaemic T-cells. They also suggest that similar differences may be found in normal T-lymphocyte subsets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01011828
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    Paper (German National Licenses)
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