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  • 1
    Electronic Resource
    Electronic Resource
    High-throughput robotic system for sequencing of microbial genomes (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 500-503 
    Details
    ISSN: 0173-0835
    Keywords: Automation ; Robotics ; DNA sequencing ; Genome ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A high-throughput robotic workstation system was used for double-stranded plasmid DNA template preparation and sequencing reaction setup to streamline the sequencing process in genome projects. All 96-well miniprep kits that were tested provided high quality plasmid DNA suitable for fluorescent DNA sequencing. After quantitation in a 96-well UV spectrophotometer, the plasmid DNA was used as template to automatically set up sequencing reactions. The setup was controlled by spread sheets that were imported into the robotic system. We utilized this integrated system to prepare all necessary shotgun templates for our contributions to a number of large-scale genome projects as well as a full-length cDNA sequencing project.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150190408
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Completion of the Saccharomyces cerevisiae Genome Sequence Allows Identification of KTR5, KTR6 and KTR7 and Definition of the Nine-Membered KRE2/MNT1 Mannosyltransferase Gene Family in this Organism (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 13 (1997), S. 267-274 
    Details
    ISSN: 0749-503X
    Keywords: mannosyltransferase ; gene family ; protein glycosylation ; cell wall ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The KRE2/MNT1 mannosyltransferase gene family of Saccharomyces cerevisiae currently consists of the KRE2, YUR1, KTR1, KTR2, KTR3 and KTR4 genes. All six encode putative type II membrane proteins with a short cytoplasmic N-terminus, a membrane-spanning region and a highly conserved catalytic lumenal domain. Here we report the identification of the three remaining members of this family in the yeast genome. KTR5 corresponds to an open reading frame (ORF) of the left arm of chromosome XIV, and KTR6 and KTR7 to ORFs on the left arms of chromosomes XVI and IX respectively. The KTR5, KTR6 and KTR7 gene products are highly similar to the Kre2p/Mnt1p family members. Initial functional characterization revealed that some mutant yeast strains containing null copies of these genes displayed cell wall phenotypes. None was K1 killer toxin resistant but ktr6 and ktr7 null mutants were found to be hypersensitive and resistant, respectively, to the drug Calcofluor White. The sequences have been deposited in the GenBank data library under Accession Numbers Z71305; U39205; Z46728.©1997 John Wiley & Sons, Ltd.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 3
    Electronic Resource
    Electronic Resource
    XI. Yeast sequencing reports. DNA sequencing and analysis of a 24·7 kb segment encompassing centromere CEN11 of Saccharomyces cerevisiae reveals nine previously unknown open reading frames (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 8 (1992), S. 749-759 
    Details
    ISSN: 0749-503X
    Keywords: DNA sequencing ; chromosome XI ; centromere CEN11 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A 24·7 kb segment of the cosmid clone pUKG047 containing a Sau3AI-partial fragment from the centromere region of Saccharomyces cerevisiae chromosome XI was sequenced and analysed. A mexed strategy of directed methods including exonuclease III nested deletion, restriction fragment subcloning and oligonucleotide-directed sequences was carried out. Exclusive use waas made of the Applied Biosystems Taq DyeDeoxy™ Terminator Cycle technology and a laser-based ABI373A sequencing system for reactions, gel electrophoresis and automated reading. A total of 12 open reading frames (ORFs) was found. Nine new ORFs (YK102 to YK110) were identified, three of which (YK102, YK107, YK108)showed homologies to proteins of known function from other organisms. In addition, sequence analysis reveled three recently functionally characterized genes (MET14, VPS/SPO15, PAP1), which could be joined to the earlier published CEN11 region.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320080908
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    Paper (German National Licenses)
    Fulltext
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