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  • 1
    Electronic Resource
    Electronic Resource
    Construction of a shuttle lacmZα-based Escherichia coli-actinomycetes vector containing the phage JHJ-3 replicon (1992)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 99 (1992), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Using the broad replicating range JHJ-3 phage replicon, a shuttle vector for Escherichia coli and actinomycetes has been constructed. The vector, pOJ31, bears the lacZα fragment allowing a blue/white gene cloning system. pOJ31 also contains a polylinker of 15 unique cloning sites and the phage T7 promoter. The vector has been used to stably express the mel gene from plasmid pIJ702 in Streptomyces lividans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1992.tb05577.x
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  • 2
    Electronic Resource
    Electronic Resource
    Chitinolytic properties of Streptomyces lividans (1991)
    Springer
    Archives of microbiology 156 (1991), S. 192-197 
    Details
    ISSN: 1432-072X
    Keywords: Streptomyces lividans ; Chitin ; Chitosan ; Chitinase ; N-Acetylglucosaminidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Streptomyces lividans TK24, an established host for genetic and molecular studies in actinomycetes, is able to use chitin as sole carbon and nitrogen source. Extracellular chitinase and N-acetyl-β-d-glucosamidinase (chitobiase) activities were detected in liquid cultures. Chitinase production was inducible by chitin and its low molecular weight derivatives. Low levels of chitinase were also produced in the absence of chitin. Production of extracellular N-acetylglucosaminidase was correlated with the beginning of the stationary phase of growth and was independent of the presence of chitin. Beside highly N-acetylated chitin, supernatants of chitin-induced cultures were able to hydrolyse chitosans with a wide range of degrees of N-acetylation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249114
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Saccharopolyspora hirsuta 367 encodes clustered genes similar to ketoacyl synthase, ketoacyl reductase, acyl carrier protein, and biotin carboxyl carrier protein (1993)
    Springer
    Molecular genetics and genomics 240 (1993), S. 146-150 
    Details
    ISSN: 1617-4623
    Keywords: Polyketide biosynthesis ; Fatty acid biosynthesis ; Biotin carboxyl carrier protein ; Carboxylase ; Saccharopolyspora hirsuta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The actI gene, encoding a component of the actinorhodin polyketide synthase of Streptomyces coelicolor, was used to identify and clone a homologous 11.7 kb BamHI DNA fragment from Saccharopolyspora hirsuta 367. The cloned fragment complemented actinorhodin production in a strain of Streptomyces coelicolor bearing a mutant actI gene. The DNA sequence of a 5.1 kb fragment revealed 6 open reading frames (ORF). ORF1 does not resemble any known DNA or deduced protein sequence, while the deduced protein sequence of ORF2 resembles that of biotin carboxyl carrier proteins. Based on the similarity to deduced protein sequences from cloned genes of polyketide producers, ORF3 would code for a ketoreductase, ORF4 and ORF5 for the putative heterodimeric β-ketoacyl synthase, and ORF6 for an acyl carrier protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276894
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    Paper (German National Licenses)
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