ISSN:
1365-2222
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Using guinea-pig lung membranes and cloned human β2-receptor adrenergic receptors the effects of whole serum, plasma, purified immunoglobulins and cellular activation products on β2-adrenergic receptor ligand binding and function were investigated. Sera from 24 non-asthmatic subjects and 115 asthmatics in different clinical categories were studied. There were no significant differences between antagonist ([125I] cyanopindolol) inhibition mediated by serum, plasma or by purified IgG when the asthmatics were compared with non-asthmatics. There was also no inhibition of 10−6m isoproterenol stimulated cAMP release from L cells expressing human β2-adrenergic receptors, by plasma, DEAE purified IgG fractions from asthmatics and non-asthmatics, or by products of activated platelets or lymphocytes. Since we have no evidence that immunoglobulins from asthmatic subjects exert functional inhibition of human β2-adrenergic receptors we conlcude that autoantibodies to the β2-adrenergic receptors do not play an important functional role in the pathophysiology of asthma.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2222.1993.tb00885.x
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