ISSN:
0021-8995
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
,
Physics
Notes:
α-Chymotrypsin has been immobilized onto partially hydrolyzed poly(ethylene)-g-co-hydroxyethyl methacrylate (120% graft), using 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide-p-toluene-sulphonate (CMC) as the activating agent. The influence of the enzyme concentration, the carbodiimide concentration, and the coupling medium on the immobilization reaction was studied. A system with 160 mg of coupled enzyme per gram copolymer, providing 126 mg of active enzyme per gram of copolymer was obtained. The KM, Vmax, and the optimum temperature profile and optimum pH value for the free and immobilized α-chymotrypsin were determined.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/app.1990.070410722
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