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  • 1
    Electronic Resource
    Electronic Resource
    The presence and distribution of glycogen particles in chondrogenic cells of the tibiotarsal anlage of developing chick embryos (1977)
    Springer
    Calcified tissue international 23 (1977), S. 45-51 
    Details
    ISSN: 1432-0827
    Keywords: Electron Microscopy ; Cytochemistry ; Glycogen ; Chondrogenesis ; Chick Embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The distribution and localization of glycogen particles in mesenchymal cells, chondroblasts and in young, hypertrophic and degenerating chondrocytes of the tibiotarsal anlage of developing chick embryos were examined ultrastructurally using the periodic acidthiocarbohydrazide-silver proteinate. The distribution of glycogen particles increased with the degree of differentiation and maturation of the chondrogenic cells. Mesenchymal cells showed minimal content of glycogen particles which gradually increased in the chondroblasts. The young chondrocytes further showed increased glycogen particle content compared to chondroblasts. This content reached a maximum level in hypertrophic chondrocytes. This level subsided in degenerating chondrocytes which showed a much reduced distribution of glycogen particles compared to hypertrophic chondrocytes. The morphological nature of glycogen particles depended on the degreee of cellular differentiation. Both mesenchymal cells and chondroblasts contained single particles, while the glycogen particles of chondrocytes aggregated and formed a series of interconnected particles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02012765
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of calcium around the membrane of the surface connected system in the human platelet (1978)
    Springer
    Histochemistry and cell biology 55 (1978), S. 271-279 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization of calcium in the membrane system of human platelets was determined by ultrahistochemical methods equipped with an electron probe x-ray microanalyzer. After potassium oxalate-glutaraldehyde treatment large amounts of electron opaque precipitates were observed around the membrane of the surface connected system. Electron probe x-ray microanalysis clearly defined that the precipitates were composed of calcium oxalate. The localization of calcium on the membrane of the surface connected system was also confirmed even after treatment of the platelets with potassium antimonate-OsO4. These results support a model which depicts the surface connected membrane system taking part in the store and the transport of calcium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00508795
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  • 3
    Electronic Resource
    Electronic Resource
    Cytochemical demonstration of amine-storing vacuoles and lysosomes in the chicken thrombocytes (1982)
    Springer
    Histochemistry and cell biology 75 (1982), S. 309-317 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A combined electron microscopic and cytochemical study of the thrombocytes of the chicken has clearly identified the amine-storing organelles and lysosomes. A chormaffin positive-reaction product was observed on the inner surface and the granules of the large electronlucent vacuoles. No acid phosphatase activity was localized in these amine-storing vacuoles. However, the acid phosphatase activity was observed in the small vesicles, the primary lysosomes, and in the large electron dense inclusions with myelin which may be secondary lysosomes. The results of this study suggest that the large empty vacuoles, with one or two very dense osmiophilic peripherally-situated granules, in the chicken thrombocytes are comparable to the vesicles with electron dense materials called “dense bodies” in mammalian thrombocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00496734
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  • 4
    Electronic Resource
    Electronic Resource
    Cytochemical evidence of the origin of the dense tubular system in the mouse platelet (1982)
    Springer
    Histochemistry and cell biology 76 (1982), S. 189-196 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Glucose-6-phosphatase (G6Pase) was used as a marker enzyme for the endoplasmic reticulum in mouse megakaryocytes and platelets. G6Pase activity was localized in the dense tubular system of the platelets. Enzyme activity was also observed in the nuclear envelope, and in the rough endoplasmic reticulum of the megakaryocytes. However, the Golgi apparatus of the megakaryocyte was never involved. The present study has added new cytochemical evidence for the hypothesis that the dense tubular system of the platelet originates from the endoplasmic reticulum of the megakaryocyte.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501921
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  • 5
    Electronic Resource
    Electronic Resource
    Precursors of monoamine-storage organelles in developing megakaryocytes of the rat (1983)
    Springer
    Histochemistry and cell biology 77 (1983), S. 353-363 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Identification and distribution of the precursors of aminestorage organelles in rat megakaryocytes during cell maturation were studied, using the uranaffin reaction for adenine nucleotide. The precursors of the amine-storage organelles appeared as 200–300 nm vesicles having an uranaffin electron dense granule, whereas they appeared as empty vesicles by conventional glutaraldehyde-OsO4 fixation. X-ray probe microanalysis confirmed the existence of U and P in the uranaffin reaction positive vesicles. The precursors appeared in the immature megakaryocytes, especially at the trans(mature) face of the Golgi apparatus, and rapidly increased in number in the maturing cells. The size of the uranaffin granules in the precursory organelles increased gradually during cell maturation and became almost equivalent to the dense body of blood platelets in the final stage of cell maturation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490898
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural distribution of peroxidase in thrombocytes of mammals and submammals (1985)
    Springer
    Histochemistry and cell biology 82 (1985), S. 345-350 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization and distribution of peroxidase (PPO) activity were studied ultracytochemically in thrombocytes from lampreys, carps, frogs, snakes, tortoises, rabbits, sheep, dogs, and monkeys. PPO activity was not deteetable in the thrombocytes of lampreys, carps, frogs, and snakes. However, this enzyme activity was demonstrated in the nuclear envelope and endoplasmic reticulum of tortoise thrombocytes. Dog and monkey thrombocytes (blood platelets) exhibited PPO activity in the dense tubular system, but this enzyme activity was not detectable in rabbit and sheep thrombocytes. Our observations are interpreted to suggest that thrombocytes from animals lower than amphibia are peroxidase negative. Furthermore, it can be said that thrombocytes from animals higher than reptiles are generally positive, although there are exceptions. PPO activity was localized in the endoplasmic-reticulum system, but not in the cytoplasmic granules of thrombocytes common to submammals and mammals. In this study, we also compared the distribution of peroxidase activity in thrombocytes, neutrophils, and eosinophils and conclude that these are significant differences in the distribution of PPO and myeloperoxidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494063
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  • 7
    Electronic Resource
    Electronic Resource
    Uptake of 3H-dopamine in megakaryocytes and blood platelets measured by quantitative electron-microscope autoradiography (1986)
    Springer
    Histochemistry and cell biology 85 (1986), S. 453-456 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We studied the uptake of dopamine by mature megakaryocytes and blood platelets in mouse spleen after a single intraperitoneal injection of 3H-dopamine. In order to compare the uptake of 3H-dopamine in mature megakaryocytes and blood platelets, we used quantitative autoradiography at the electron-microscope level. Dense accumulations of silver grains were observed on both mature megakaryocytes and blood platelets; all other tissue elements of the spleen exhibited considerably less dense labeling. No sigificant differences with regard to dopamine uptake were observed in megakaryocytes and blood platelets. This is in contrast to the previous finding of very different patterns of 3H-5-hydroxytryptamine labeling in mature megakaryocytes and blood platelets (Daimon and Uchida 1985). The results of the present study provide new evidence in favor of the hypothesis that the active uptake mechanism of dopamine through the plasma membrane is different from the uptake mechanism of 5-hydroxytryptamine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00508426
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  • 8
    Electronic Resource
    Electronic Resource
    Location of sites of dopamine storage in megakaryocytes by autoradiography (1990)
    Springer
    Histochemistry and cell biology 93 (1990), S. 263-267 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We have used quantitative electron microscope autoradiography to study the subcellular sites of 3H-dopamine uptake in mouse megakaryocytes after a single intraperitoneal injection. Autoradiographic grains were found to be associated almost exclusively with the vesicles of precursors of monoamine-storage organelles. The labeling intensity (radioactivity) of the demarcation membrane system which is continuous with the plasmalemma was also significantly greater than would be expected. On the other hand, radioactivity associated with the remaining sites in the cytoplasm was not significantly different from that expected of a random distribution. In order to compare 3H-dopamine uptake during cell maturation, light microscope autoradiographic studies were also done. Immature megakaryocytes were labeled slightly, but the number of silver grains increased significantly in maturing cells. Mature megakaryocytes were 2.7 times more radioactive than the maturing cells. Our results suggest that the megakaryocytes were able to accumulate dopamine from the early stages of cell maturation and to store dopamine in precursors of monoamine-storage organelles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266387
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  • 9
    Electronic Resource
    Electronic Resource
    Elemental composition of the dense bodies of rat platelets determined by electron probe X-ray microanalysis of freeze-substituted sections (1983)
    Springer
    Histochemistry and cell biology 77 (1983), S. 329-337 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A semiquantitative x-ray microanalytical study was made of dense bodies in rat and human blood platelets prepared by freeze substitution. After the freeze substitution, electron dense bodies containing 5-hydroxytryptamine(5-HT) were well preserved. Electron probe x-ray microanalyses clearly defined species differences in the cations of dense bodies of rat and man. Direct calculation of elemental concentration ratios demonstrated a high level of Mg, with lesser amounts of K and Ca in rat, while there was a negligible level of Mg in man but a high amount of Ca. These results indicate that freeze-substitution is a suitable preparation for the study of diffusible electrolytes which may open the way to quantitative analysis using appropriate standards for analytical electron microscopy of resin sections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490896
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  • 10
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of acid protein polysaccharides and calcium in the vacuoles of the chicken thrombocyte (1977)
    Springer
    Histochemistry and cell biology 52 (1977), S. 25-32 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The coexistence of acid protein polysaccharides and calcium in the vacuoles of chicken thrombocytes were studied by means of ultrahistochemical methods and electron probe X-ray microanalysis. The thrombocytes possessed large vacuoles of a surface connected membrane system. After both ruthenium red staining and tannic acid fixation the innersurface coat of vacuoles was always strongly and continuously visualized. Electron microscopic X-ray microprobe analysis of antimonate precipitates in thrombocytes fixed in K-antimonate-OsO4 revealed calcium localization on the innersurface of vacuoles. From these facts it seems likely that the vacuoles of the surface connected membrane system may take part in the pool or the transport of calcium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495811
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