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  • 1
    Electronic Resource
    Electronic Resource
    Transformation of Medicago arborea L. with an Agrobacterium rhizogenes binary vector carrying the hygromycin resistance gene (1991)
    Springer
    Plant cell reports 10 (1991), S. 300-303 
    Details
    ISSN: 1432-203X
    Keywords: Transformation ; Agrobacterium rhizogenes ; forage legumes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plants of Medicago arborea have been infected with Agrobacterium rhizogenes strain LBA9402 harbouring the plasmids Ri 1855 and AGS125 carrying a gene conferring resistance to the antibiotic hygromycin. About 7056 of the hairy roots showed callus formation on hygromycin-supplemented medium. Regeneration took place on antibiotic free medium only. Plantlets suitable for transfer to soil were obtained after the manual removal of most of the leaves. Plant morphology showed the usual alterations induced by the Ri plasmid; moreover, two years after soil-transfer, transformants have not flowered. Molecular analysis indicates the presence of T-DNA from both pAGS 125 and p1855. The expression of the hygromycin phosphotransferase gene allowed callus and protoplasts of transformed plants to grow on media supplemented with the antibiotic. This trait will be utilized as a marker in protoplast fusion between Medicago arborea and Medicago sativa (alfalfa).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193146
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Introduction of hygromycin resistance inLotus spp. throughAgrobacterium rhizogenes transformation (1993)
    Springer
    Transgenic research 2 (1993), S. 330-335 
    Details
    ISSN: 1573-9368
    Keywords: Lotus ; Agrobacterium rhizogenes ; transformation ; hygromycin resistance ; tannins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The speciesLotus corniculatus andL. tenuis were transformed with anAgrobacterium rhizogenes binary vector, conferring resistance to the antibiotic hygromycin. Transgenic plants recovered from both species were tested for the ability of leaf-derived calluses to grow in a hygromycin-supplemented medium. Molecular analysis showed the integration of the Ri T-DNA and of the gene for hygromycin resistance, with a high frequency of co-transformation. Progeny analysis of the hygromycin resistance indicated this to be a single Mendelian trait in test plants. The transformed plants will be utilized in somatic hybridization experiments with lucerne for producing non-bloating genotypes with condensed tannins in leaves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01976174
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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