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  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructural cytochemistry of the secretory granules of the hamster submandibular gland (1977)
    Springer
    Histochemistry and cell biology 51 (1977), S. 261-268 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure and cytochemistry of the secretory granules of the male hamster submandibular salivary gland were studied. After fixation in glutaraldehyde followed by osmium tetroxide the granules exhibit a characteristic bipartite substructure, with an electron lucid crescenteric rim and a more dense central core. A differentiation into two regions of the granules could also be visualized in specimens primarily fixed in Millonig's osmium tetroxide or in potassium permanganate. The electron lucid peripheral portion of the membrane bounded secretory granules further displays a strong positive reaction after staining of ultrathin sections with the periodic acid-chromic acid-(PA-CrA)-silver technique. The strong periodate reactivity of the rim relative to the core, suggests a difference in mucin composition of the two granule regions. With the PA-CrA-silver staining technique a positive reaction was also observed within the Golgi apparatus of the acinar cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494362
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Peroxidase and amylase activity of the guinea pig submandibular gland during the secretory cycle (1974)
    Springer
    Histochemistry and cell biology 38 (1974), S. 271-280 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Endogenous peroxidase activity was studied at the electronhistochemical level at various stages of the secretory cycle in the guinea pig submandibular gland. The histochemical findings were correlated to quantitative assays of peroxidase and amylase activity in the glands. Electron microscopy revealed that peroxidase reaction product was localized to the rough endoplasmic reticulum, including the perinuclear cisternae and to the secretory granules of the acinar cells. The reaction of the Golgi apparatus was ambiguous. A few minutes after induction of secretion, granule discharge was observed, as was the presence of peroxidase-positive material within the acinar lumina. Ninety minutes after injection of a combination of pilocarpine and isoprenaline the acinar cells were depleted of secretory granules and the amylase activity had decreased by roughly 75 per cent and the peroxidase activity by 50 per cent. Six hours after the injection, reaccumulation of peroxidase-positive granules was observed. Twenty-four hours after administration of the drugs the acinar cells were completely refilled with cytoplasmic granules displaying positive peroxidase staining. At this stage the enzyme concentrations of the glands were restored to the levels of non-treated control animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493125
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of glucose, insulin and glucagon on amylase secretion from incubated mouse pancreas (1974)
    Springer
    Pflügers Archiv 348 (1974), S. 333-342 
    Details
    ISSN: 1432-2013
    Keywords: Mouse Pancreas ; Amylase ; Glucose ; Insulin ; Glucagon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Glucose inhibited amylase release from batch-incubated and perifused pieces of mouse pancreas. Insulin (100μg/ml) reduced the amylase secretion. These effects, however, seemed to be independent of each other, since diazoxide, a potent inhibitor of glucose-induced insulin release, did not abolish the effect of glucose on amylase secretion. Glucagon (10–100 μg/ml) had no effect on the release of amylase from incubated pancreas. Glucagon has previously been shown to inhibit exocrine secretionin vivo. The present results suggest that the inhibitory action of glucagon may be mediated via an increased level of serum glucose and the subsequent release of insulin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00589222
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of cholecystokinin-pancreozymin (CCK-PZ) on glycoprotein secretion from mouse gallbladder epithelium (1976)
    Springer
    Cell & tissue research 171 (1976), S. 425-435 
    Details
    ISSN: 1432-0878
    Keywords: Cholecystokinin-pancreozymin (CCK-PZ) ; Glycoprotein secretion ; Mouse gallbladder ; Exo-endocytosis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Structural changes in the gallbladder epithelial cells of the mouse were studied following in vivo and in vitro stimulation of the gallbladder with the gastrointestinal hormone cholecystokinin-pancreozymin (CCK-PZ). Signs of increased secretory activity were observed within the first 2–3 min after hormone administration. At the ultrastructural level, best visualized with the PA-CrA-silver technique, granule discharge was observed, as was an overall increase in size of the granules. After prolonged in vitro incubation or repeated in vivo stimulation, there was an almost total depletion of secretory granules. This phenomenon is accompanied by an enhanced uptake of extracellular thorium dioxide by endocytotic vesicles at the apical cell surface. An exocytosisendocytosis coupling mechanism may be important for membrane conservation in the gallbladder epithelial cells. The findings establish that the hormone CCK-PZ stimulates the secretion of glycoproteins from the mouse gallbladder epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220235
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Impact of gastrointestinal dysfunction on survival after liver transplantation for familial amyloidotic polyneuropathy (1996)
    Springer
    Digestive diseases and sciences 41 (1996), S. 1909-1914 
    Details
    ISSN: 1573-2568
    Keywords: hereditary amyloidosis ; inborn errors of metabolism ; malabsorption ; fat ; bile acids ; liver transplantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Liver transplantation is the only effective treatment of familial amyloidotic polyneuropathy type I (FAP). The aim of the present investigation was to identify factors at the time of submission for transplantation that had impact on survival, with special reference to gastrointestinal disturbances. All 28 liver-transplanted FAP patients evaluated at Umeå University Hospital were included in the study. A modified body mass index was used to assess nutritional status. Intestinal examinations were performed to diagnose bile acid malabsorption, gastric retention, and bacterial contamination of the small bowel. A significantly improved survival rate was found for patients in a good nutritional state (P=0.002). Peripheral neurological symptoms were unrelated to survival, whereas increased mortality was found for patients with bile acid malabsorption (P〈0.05). Bacterial contamination and gastric retention were common complications of the disease. In conclusion, malabsorption and malnutrition have a profound impact on the outcome of liver transplantation for familial amyloidotic polyneuropathy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02093589
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Trophic effect of the sympathetic nervous system on the early development of the rat parotid gland: A quantitative ultrastructural study (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 201 (1981), S. 645-654 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Rats were sympathetically denervated on one side by avulsion of the superior cervical ganglion either immediately after birth (within 4 hr) or when the salivary glands were fully developed. Nine weeks after ganglionectomy the parotid glands were subjected to microscopical studies. As shown by the lack of specific fluorescence, sympathetic denervation caused an almost total depletion of catecholamines in the acini. This was further substantiated at the electron microscopic level using KMnO4 as fixative. No alterations in either gland weight or in acinar cell size were noticeable after adult sympathectomy. On the other hand, neonatal denervation caused a decrease in gland weight as well as acinar cell hypotrophy. The mean volume of individual acinar cells was reduced by roughly 25% and the granule volume density by about 50%. Also the mean volume of individual granules was decreased. These findings indicate an important role for the sympathetic nerve system in the maturation of the rat parotid gland.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092010409
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    Paper (German National Licenses)
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