ISSN:
1573-5028
Keywords:
acyl-CoA-binding protein
;
Brassica napus
;
diazepam-binding inhibitor protein
;
linkage map
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A cDNA encoding an acyl-CoA-binding protein (ACBP) homologue has been cloned from a λgt11 library made from mRNA isolated from developing seeds of oilseed rape (Brassica napus L.). The derived amino acid sequence reveals a protein 92 amino acids in length which is highly conserved when compared with ACBP sequences from yeast, cow, man and fruit fly. Southern blot analysis ofBrassica napus genomic DNA revealed the presence of 6 genes, 3 derived from theBrassica rapa parent and 3 fromBrassica oleracea. Northern blot analysis showed that ACBP genes are expressed strongly in developing embryo, flowers and cotyledons of seedlings and to a lesser extent in leaves and roots.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00028886
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