ISSN:
1420-9071
Keywords:
Hybridization histochemistry
;
hybridocytochemistry
;
in situ hybridization
;
gene expression
;
histochemical hybridization
;
nucleic acid hybridization
;
histocytochemistry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The location of gene expression by hybridization histochemistry is being applied in many areas of research and diagnosis. The aim of this technique is to detect specific mRNA in cells and tissues by hybridization with a complementary DNA or RNA probe. Requirements for optimal specificity, sensitivity, resolution and speed of detection may not all be encompassed in one simple technique suitable for all applications, thus appropriate procedures should be selected for specific objectives. With reference to published procedures and our own extensive experience, we have evaluated fixatives, probes, labels and other aspects of the technique critical to the preservation and hybridization in situ of mRNA and detection and quantition of hybrids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01945351
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