ISSN:
1432-0843
Keywords:
Key words Multidrug resistance
;
P-glycoprotein
;
MRP
;
DACA
;
Idarubicin
;
Paclitaxel
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The effectiveness of N-[2-(dimethylamino) ethyl]acridine-4-carboxamide (DACA) relative to that of amsacrine, idarubicin, daunorubicin and paclitaxel against three different forms of multidrug resistance (MDR) was determined using two sublines of the CCRF-CEM human leukaemia cell line, the P-glycoprotein-expressing CEM/VLB100 subline and the MRP-expressing CEM/E1000 subline, and two extended-MDR sublines of the HL60 human leukaemia cell line, HL60/E8 and HL60/V8. DACA was effective against P-glycoprotein-mediated MDR and MRP- mediated MDR, whereas the extended-MDR pheno- type showed only low levels of resistance (〈2-fold) to DACA. In comparison, idarubicin was ineffec- tive against the MRP and extended-MDR phenotypes. Repeated exposure of the K562 human leukaemia cell line to DACA (55, 546 or 1092 nM for 3 days over 10 weeks) did not result in the development of any significant drug resistance. We conclude that DACA has the potential to treat refractory leukaemia.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002800050593
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