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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Community dentistry and oral epidemiology 3 (1975), S. 0 
    ISSN: 1600-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A partial recording system, suitable for use in caries incremental studies, was developed from first principles using data from 330 11- to 14-year-old English schoolchildren. This system was shown to be rapid, accurate and highly efficient when the results were compared with full-mouth statistics. Comparisons were also made with five published caries partial recording methods, using 2- and 3-year incremental data from a recent clinical trial. The results have been calculated both excluding and including precavitation lesions, as well as for three separate surface types (fissure, free smooth surface and approximal). Three methods of expressing “relative efficiency” are compared. The need for partial recording systems to be maximally efficient is discussed. The results confirm that many partial recording techniques are accurate, efficient and capable of considerably reducing examination time.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 25 (2002), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: China's soils tend to be phosphate deficient. Application of phosphorus fertilisers to the soil is yield and cost ineffective as much of the phosphate applied is rapidly locked-in and is inaccessible to the crop. Chinese Institutes have established intensive wheat breeding programmes to generate wheat varieties that produce adequate yields and grain quality in such soils. Three such wheat cultivars have been identified with good performance characteristics in the field. These three cultivars are thought to harbour chromosome translocations that may confer enhanced phosphate scavenging abilities to the plants. The isolation and study of the expression of high-affinity phosphate transporters in tissues of these wheats, in two of the donor wheatgrasses and in another widely planted Chinese wheat variety is presented and the first full-length sequence of a wheat phosphate transporter and partial clones of several other putative phosphate transporters are reported. Relative quantitative reverse-transcription – polymerase chain-reaction was used to demonstrate that different phosphate transporters have different expression patterns within a given variety and respond differently to phosphate deprivation. The significance of the genetic background for these findings and for the different phosphate acquisition properties of the wheats under study is discussed.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 23 (2000), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Solute transport systems are one of the major ways in which organisms interact with their environment. Typically, transport is catalysed by integral membrane proteins, of which one of the largest groups is the ATP-binding cassette (ABC) proteins. On the basis of sequence similarities, a large family of ABC proteins has been identified in Arabidopsis. A total of 60 open reading frames (ORFs) encoding ABC proteins were identified by BLAST homology searching of the nuclear genome. These 60 putative proteins include 89 ABC domains. Based on the assignment of transmembrane domains (TMDs), at least 49 of the 60 proteins identified are ABC transporters. Of these 49 proteins, 28 are full-length ABC transporters (eight of which have been described previously), and 21 are uncharacterized half-transporters. Three of the remaining proteins identified appear to be soluble, lacking identifiable TMDs, and most likely have non-transport functions. The eight other ORFs have homology to the nucleotide-binding and transmembrane components of multi-subunit permeases. The majority of ABC proteins found in Arabidopsis can, on the basis of sequence homology, be assigned to subfamilies equivalent to those found in the yeast genome. This assignment of the Arabidopsis ABC proteins into easily recognizable subfamilies (with distinguishable subclusters) is an important first step in the elucidation of their functional role in higher plants.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Community dentistry and oral epidemiology 11 (1983), S. 0 
    ISSN: 1600-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract 751 14- and 15-year old children completed a 3-year, double-blind, caries preventive program. The effects of daily, supervised toothbrushing with an 0.76% sodium monofluorophosphate dentifrice, rinsing with a 0.05% sodium fluoride mouthrinse, and the combined effects of the two treatments were investigated. Both the dentifrice and mouthrinse reduced the incidence of dental caries, but their combined use at die same lime had no greater effect than either used alone.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 224 (1969), S. 553-560 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Eruptions, apparently associated with the caldera fault zone, have created a new island and a new 5 km fissure. Several explosive vents along the fissure caused a rift in the overlying glacier releasing a flood of water carrying ice ...
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 79 (1990), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Gradients of gene expression in the developing fourth leaf of the grass Lolium temulentum L. strain Ba 3081 were investigated using cell-free translation and Northern analysis of RNA extracted from succesive leaf segments. The transition from growth at the leaf base to photosynthesis in the blade was reflected in relative levels of transcripts from the plastid genome (psb A, pet A and rbc L, encoding plastid polypeptides) and nuclear genome (cab and PCR, encoding plastid polypeptides, and histone H3 and PAL encoding extra-plastidic polypeptides). These data combined with information on polypeptide synthesis in vivo indicate that post-transcriptional regulation plays an important part in controlling gene expression in this tissue.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cellular and compositional changes related to tissue growth and assembly of the photosynthetic apparatus were established with reference to the developmental gradient along the expanding fourth leaf of Lolium temulentum L. Ba 3081. The number of cells and the fresh weight per leaf segment fell with increasing cell age (distance from the leaf base). Components of the photosynthetic apparatus increased in concentration towards the more mature part of the leaf. Appreciable levels of chlorophyll and carotenoid could be detected in basal tissue enclosed in the sheaths of previous leaves prior to emergence into full light. The distributions of NADPH-protochlorophyllide oxidoreductase (PCR. EC 1.6.99.1) and cytochrome f along the age gradient were visualized by Western blotting. Leaf base tissue contained two forms of PCR, 41 and 39 kDa, the smaller of which diminished with increasing cell age and proximity to full light. Cytochrome f comprised a 52 kDa species, which also declined with distance from the base, and a group of polypeptides at around 30–33 kDa which greatly intensified with tissue maturity. The significance of multiple forms of plastid proteins and the role of the light gradient penetrating the leaf sheath in regulating growth and plastid assembly processes are discussed.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-2048
    Keywords: Avena (aleurone) ; Membrane protein (microsomal) ; Protoplast ; Tonoplast intrinsic Protein Vacuole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cultured oat (Avena sativa L. — naked form) aleurone protoplasts were employed as a model system for following changes which accompany the development of vacuoles during in-vitro incubation. Over a 5-d period, the aleurone grains progressively grew and fused to form a large central vacuole and the volume of the protoplasts increased sevenfold. The growth of the vacuole was accompanied by a progressive acidification of the vacuolar sap. Vacuolation was inhibited by high concentrations of mannitol and by cycloheximide and cordycepin applied at various times during the incubation period. Neither cycloheximide nor cordycepin affected the initial phases of vacuolation but cycloheximide retarded subsequent stages, particularly if added early in the incubation. Cordycepin inhibited only the later stages of vacuolation. Radiolabelling studies identified at least three novel microsomal proteins, with relative molecular masses of approximately 34, 47 and 48 kDa, which appeared during vacuolation and whose synthesis was markedly affected by these inhibitors.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-5079
    Keywords: cytochrome f ; ELISA ; Festuca pratensis ; senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A non-competitive enzyme-linked immunosorbent assay (ELISA) which enables detection of as little as 0.1 ng cytochrome f in leaf extracts has been developed. No evidence for specific or non-specific interference by proteins other than cytochrome f was found. The assay was applied to a comparative study of age-related changes in the cytochrome f content of leaves of Festuca pratensis Huds. cv. Rossa, and a non-yellowing mutant genotype (Bf993) having a lesion in the mechanism responsible for thylakoid membrane disassembly. Cytochrome f in senescent leaves of the latter genotype was found to be present at significantly higher levels than in the wild-type, implying an inability on the part of the mutant to degrade this protein. The results obtained by ELISA were confirmed by antibody probing of Western blots.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Communications in Numerical Methods in Engineering 11 (1995), S. 927-935 
    ISSN: 1069-8299
    Keywords: incompressible continuum ; fundamental solutions ; boundary element method ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Novel fundamental solutions for elastodynamics of fully incompressible media are derived, by reason of their many applications to engineering and physical problems. It is demonstrated that, when Poisson's ratio equals 1/2, the frequency domain fundamental solutions (Stokes's solutions) are independent of the dilatational wave velocity, which tends to infinity. Consequently, dynamic problems involving incompressible materials can be rigorously analysed by the boundary element method without special difficulty. Numerical solutions to geotechnical problems of incompressible half-spaces are presented to demonstrate the applicability and accuracy of the formulations.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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