ISSN:
1573-2592
Keywords:
B cells
;
abnormalities
;
HIV
;
viral load
;
plasma viremia
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Many B cell abnormalities have been reported in human immunodeficiency virus (HIV)-infected patients, including changes in the expression of μ, γ, and CD22 molecules on the cell surface. Phenotypic changes in these markers on B cells isolated from HIV-seropositive patients with high or low levels of plasma viremia were measured. The phenotypic changes in B cells isolated from such patients were compared with the markers on B cells isolated from HIV-seronegative individuals using three-color flow cytometry. HIV patients showed a reduction in the proportion of mature B cells isolated from peripheral blood mononuclear cells compared with B cells isolated from HIV-seronegative individuals. An increase in the proportion of B cells expressing both μ and γ molecules on the cell surface was also seen in association with high-HIV plasma viremia. A low plasma viral load was accompanied by a reduction in the proportion of B cells expressing both μ and γ molecules to a level comparable to those seen in HIV-seronegative individuals. HIV-seropositive individuals demonstrated an increase in the proportion of committed B cells, as indicated by an increase in the proportion of B cells expressing γ molecules. This observation may explain the poor humoral response of HIV seropositive patients to neo-antigens. Our results demonstrate that phenotypic changes indicative of in vivo B cell activation and an increase in immature cells are associated with HIV infection, particularly with a high plasma viral load. Phenotypic changes in B cell markers may correlate with functional deficits of B cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1020539207593
Permalink
