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  • 1
    Electronic Resource
    Electronic Resource
    Chemiluminescence of mononuclear cells is enhanced during antigen recognition (1993)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 8 (1993), S. 159-167 
    Details
    ISSN: 0884-3996
    Keywords: Mononuclear cells ; chemiluminescence ; antigen stimulation ; interferon-gamma ; T lymphocytes ; Candida albicans ; Rabies (Lyssa) virus ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Stimulation of phagocytes by several cytokines causes superoxide generation and consequently chemiluminescence. Since antigen-activated lymphocytes generate cytokines, we investigated whether antigen recognition by mononuclear cells, which contain both lymphocytes and monocytes, is accompanied by changes in lucigenin-dependent chemiluminescence. Mononulcear cells which underwent antigen-induced proliferation showed a delayed rise in lucigenin-dependent chemiluminescence in the absence of other stimuli. The common recall antigen Candida albicans increased spontaneous chemiluminescence of mononuclear cells from unselected donors up to 20-fold over control values after 48-72h of culture. With Rabies virus vaccine as specific antigenic stimulus, only mononuclear cells from rabies immunized individuals responded with enhanced delayed chemiluminescence. In contrast to opsonized zymosan and phorbol myristate acetate, antigens induced no oxidative burst within one hour after addition. Delayed mononuclear cel chemiluminescence was inhibited by the superoxide scavenger superoxide dismutase and by di-phenylene iodonium, a selective inhibitor of the phagocyte NADPH oxidase. A neutralizing monoclonal antibody against interferon-gamma completely abrogated antigen-induced chemiluminescence. Recombinant interferon-gamma by itself induced delayed mononuclear cell chemiluminescence. Thus, antigen-induced delayed mononuclear cell chemiluminescence represents activation of phagocyte NADPH oxidase by interferon-gamma generated by activated lymphocytes.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170080306
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  • 2
    Electronic Resource
    Electronic Resource
    Aspects of Formation of the D-Penicillamine-Antigenic Determinant from Penicilloyl Compounds (1973)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 56 (1973), S. 1235-1243 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The formation of D-penicillamine-L-cysteine mixed disulfide from benzylpenicilloic acid, benzylpenilloic acid and benzylpenicilloyl amide derivatives in L-cysteine suspensions at pH 7,6 and 37° was studied. The reaction is faster than direct penicilloylation of proteins known to be a route to penicilloyl antigenic determinants. The production of S-bound penicillamine on protein from penicilloyl compounds must therefore definitely be considered as a potential antigenforming step. The reaction may be partly if not fully blocked by acylation of the thiazolidine nitrogen of penicilloyl compounds. When formylation is applied a considerable reduction of the capacity of penicilloyl antigenic determinants to interact with anti-penicilloyl antibody is noted. A D-penicillamine-specific test antigen was prepared by binding D-penicillamine via thioether links to a partly succinylated poly-L-lysine. Clinical test reactions elicited with this conjugate and with penicilloic acid cannot be well correlated. Penicilloic acid probably detects reactions of undefined specificity in addition to D-penicillamine-specific ones.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19730560408
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Epitope analysis: N4-Formylation of the D-benzylpenicilloyl antigenic structure (1991)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1991 (1991), S. 85-87 
    Details
    ISSN: 0170-2041
    Keywords: Epitope analysis ; Penicillin antigens ; Thiazolidines, formylated ; Mutarotated penicilloyl ; Benzylpenicilloic acid ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Acetic anhydride treatment of 6-(benzylpenicilloylamido)-hexanoic acid bis(benzylammonium) salt (1a) in formic acid gave the N4-formylated derivatives 2a and 2b as diastereomeric mixtures. The inhibitory capacity of these haptens was assessed in a precipitating system involving anti-D-α-benzylpenicilloyl antibody and D-α-benzylpenicilloyl antigen.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.199119910116
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    Paper (German National Licenses)
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