ISSN:
1573-8221
Keywords:
multiple resistance to antibiotics
;
cloning
;
modification
;
restriction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Cells ofEscherichia coli SK are not colicinogenic but they possess multiple resistance to antibiotics (tetracycline, kanamycin, penicillin, polymixin, ampicillin). By cloning of the original culture the whole series of variants sensitive to one or more of the abovementioned antibiotics was obtained. Resistance of theE. coli SK cells to all five antibiotics could not be completely removed with acridine orange. The partial changes in the spectrum of antibiotic resistance did not affect the system of host specificity present in the SK cells. Capacity for restriction and activity of methylating enzymes were identical in all the clones studied and in the original strain.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00801989
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