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  • 1
    Electronic Resource
    Electronic Resource
    The Microcirculation of the Testis (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 564 (1989), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1989.tb25901.x
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic selection for reproductive photoresponsiveness in deer mice (1986)
    [s.l.] : Nature Publishing Group
    Nature 322 (1986), S. 172-173 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In the northern United States and southern Canada, members of the rodent genus Peromyscus usually exhibit a well-defined breeding season. Reproduction is largely limited to the spring and summer when food is plentiful, the climate benign and photoperiod long2'3. Several northern populations of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/322172a0
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  • 3
    Electronic Resource
    Electronic Resource
    Hypophyseal LH and FSH release and uterine nucleic acid changes during the mouse estrous cycleThis investigation was supported in part by an allocation from Public Health Service General Research Support grant FR-05545-05 to The Jackson Laboratory, in part by Public Health Service Training grant T1CA-5013 from the National Cancer Institute, and in part by Public Health Service Research grant HD-00767 from the National Institute of Child Health and Human Development. The principles of laboratory animal care as promulgated by the Council of the American Physiological Society are observed in this laboratory. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 167 (1970), S. 465-471 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Concentrations of hypophyseal and plasma luteinizing hormone (LH) and follicle stimulating hormone (FSH) were measured along with the total amount of uterine DNA and RNA during four stages of the mouse estrous cycle: proestrus, estrus, metestrus, and diestrus. Female mice were exposed to male olfactory stimuli to induce repeatable 4-5 day estrous cycles. The mean concentration of hypophyseal LH dropped from 2.9 μg/mg at diestrus to 0.3 μg/mg at proestrus. During this same time period the concentration of plasma LH increased from 3.3 μg/100 ml to 10.6 μg/100 ml. In contrast to the fluctuations in LH, the concentration of FSH in the hypophyses and plasma remained relatively constant throughout the estrous cycle. Uterine weight and total uterine DNA, RNA, and the ratio of RNA:DNA were all significantly greater (P 〈 0.05) at proestrus than at metestrus suggesting significant fluctuations in cell numbers and protein synthetic activity of the uterus during the estrous cycle. The fluctuations in LH and FSH release and in uterine nucleic acids noted during the mouse estrous cycle generally agree with observations in laboratory rats. However, the pattern of LH and perhaps FSH release preceding ovulation in mice may be different than that suggested for rats. These differences may be attributed to the greater dependence of mice on male olfactory stimuli.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091670407
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  • 4
    Electronic Resource
    Electronic Resource
    The cycle of the seminiferous epithelium and spermatogenesis in the bovine testisThis investigation was supported in part by U. S. Public Health Service Research grant HD-04578 from the National Institute of Child Health and Human Development and in part by an allocation from the Oklahoma State University Agricultural Experiment Station. Journal article J-2587 of the Agricultural Experiment Station, Oklahoma State University, Stillwater, Oklahoma 74074. Portions of this work were presented at the 5th Annula Meeting of the Society for the Study of Reproduction, June, 1972. (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 140 (1974), S. 167-179 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Morphological changes in the acrosomic system and nuclei of developing spermatids were evaluated as a basis for classifying the stages of the cycle of the seminiferous epithelium in the bovine testis. Light microscopic examination of periodic acid-Schiff-stained testicular tissue permitted identification of 14 steps of spermatid development (spermiogenesis). The first 12 steps in this sequence were utilized as the major criterion to divide the cycle of the seminiferous epithelium into 12 distinct stages. Following this, the pattern of germ cell differentiation was investigated by counting the number of germ cells at each stage of the cycle. Based on cell counts, type A spermatogonia divided primarily during stages VII-VIII and IX-X of the cycle. Some type A cells divided again at the end of stage XII to produce intermediate spermatogonia, while others apparently remained “dormant” until the following cycle. At the end of stage IV, intermediate spermatogonia divided to produce type B1 spermatogonia which in turn divided at the end of stage V to produce type B2 spermatogonia. Primary spermatocytes appeared during stage VIII and divided late in stage XI of the following cycle to form secondary spermatocytes. These divided to form young spermatids at the end of stage XII. It was concluded that changes in the acrosomic system and nuclear morphology of developing spermatids provide useful criteria for dividing the cycle of the seminiferous epithelium into stages as well as investigating the pattern of germ cell development during spermatogenesis in the bovine testis.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001400204
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  • 5
    Electronic Resource
    Electronic Resource
    Vasculature of the mouse, rat, and rabbit testis-epididymis (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 165 (1982), S. 357-372 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The arrangement of blood vessels serving the testis-epididymis was investigated microscopically in the mouse, rat, and rabbit. Blood vessels were visualized by infusing liquid silicone rubber into the vessels and subsequently clearing the surrounding tissue. Comprehensive illustrations of the vasculature were prepared from three-dimensional examinations.Arterial and venous vessels serving the testis-epididymis follow similar routes in all three species. However, the arrangements and characteristics of the blood vessels demonstrate dramatic species differences. For example, arteries within the testes have tight coils in the mouse and artery-artery anastomoses in the rat. Veins form vascular pathways that connect the testis and efferent ductules in all three species but also form a connection between the testis and cauda epididymidis in the rabbit only.Testosterone concentrations were determined in blood obtained by micropuncture of selected testis-epididymal veins. The measurements establish that the highest levels of testosterone are found in testicular surface veins. Also, vas deferential veins of the rabbit had significant amounts of testosterone. Studies of the blood-vessel volumes suggested that the volumes of arteries and veins in the testis are similar, whereas venous volumes exceed arterial volumes in all of the other organs examined.The studies provide comprehensive information about the architecture and physiology of blood vessels serving the testis-epididymis in the mouse, rat, and rabbit. Each species exhibits diversity in the vasculature and testosterone content of the veins. Veins connecting the testis to the efferent ductules and cauda epididymidis may provide for the preferential delivery of testicular secretions to androgen-dependent organs before the secretions are metabolized or diluted in the systemic blood.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001650402
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  • 6
    Electronic Resource
    Electronic Resource
    Active cell aggregation by immature rat Sertoli cells in primary culture: A role for cell surface glycoproteins (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 99 (1979), S. 175-182 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Follicle stimulating hormone (FSH) stimulates “colony formation” by immature rat Sertoli cells in primary culture. “Colony formation” involves cell aggregation. Consequently, the involvement of cell surface glycoproteins in cell aggregation was investigated by treatment of dissociated 10-day rat testis cells with sodium metaperiodate, glucosamine, various lectins, tunicamycin, and puromycin. Treatment of control cultures with 5 μM glucosamine stimulated cell aggregation; however, glucosamine did not affect FSH-stimulated cultures. Treatment of dissociated testis cells with 5 μM sodium metaperiodate, 10 μg/ml castor bean agglutinin (ricin), or 2.5 μg/ml horseshoe crab agglutinin inhibited FSH stimulation of cell aggregation. A similar inhibition of cell aggregation was observed following addition of 10 μg/ml puromycin or tunicamycin to culture media from 0- to 18-hours incubation. Treatment with soybean agglutinin, concanavalin A, or wheat germ agglutinin had no effect. The galactose-specific lectins, Ricin, Ricinus communis agglutinin I, and Bendeirea simplicifolia agglutinin, inhibit the FSH stimulation of 3H-aminoacid incorporation as well as cell aggregation in 24-hour cultres. The inhibition of cell aggregation by sodium metaperiodate treatment was reversed with 5 μM sodium borohydride reduction. Sodium metaperiodate treatment did not alter cell viability (as assayed with trypan blue dye exclusion), did not alter cell attachment, nor significantly decrease 125I-FSH binding by cultured testis cells. The results suggest that FSH stimulation of cell aggregation by immature rat Sertoli cells requires cell surface glycoprotein interactions. Furthermore, the specificity of lectin inhibition suggests that glycoproteins with terminal galactose and sialic acid residues are required for the FSH induction of cell aggregation.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040990203
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