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  • 1
    Electronic Resource
    Electronic Resource
    Analytical conditions for the determination of 23 phenylthiocarbamyl amino acids and ethanolamine in musts and wines by high-performance liquid chromatography
    Amsterdam : Elsevier
    Journal of Chromatography A 685 (1994), S. 21-30 
    Details
    ISSN: 0021-9673
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0021-9673(94)00674-1
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  • 2
    Electronic Resource
    Electronic Resource
    Assessment of Genetic Diversity in Three Subsets Constituted from the ICRISAT Sorghum Collection Using Random vs Non-Random Sampling Procedures B. Using molecular markers (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 197-202 
    Details
    ISSN: 1432-2242
    Keywords: Key words Core collection ; Sorghum ; SSRs ; Genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The large size of the sorghum [Sorghum bi-color (L.) Moench] landrace collection maintained by ICRISAT lead to the establishment of a core collection. Thus, three subsets of around 200 accessions were established from: (1) a random sampling after stratification of the entire landrace collection (L), (2) a selective sampling based on quantitative characters (PCS), and (3) a selection based on the geographical origin of landraces and the traits under farmers’ selection (T). An assessment was done of the genetic diversity retained by each sampling strategy using the polymorphisms at 15 microsatellite loci. The landraces of each subset were genotyped with three multiplex polymerase chain reactions (PCRs) of five fluorescent primer-pairs each with semi-automated allele sizing. The average allelic richness for each subset was equivalent (16.1, 16.3 and 15.4 alleles per locus for the subsets PCS, L, and T, respectively). The average genetic diversity was also comparable for the three subsets (0.81, 0.77 and 0.80 for the subsets PCS, L, and T, respectively). Allelic frequency distribution for each subset was compared with a chi-square test but few significant differences were observed. A high percentage of rare alleles (71 to 76% of 206 total rare alleles) was maintained in the three subsets. The global molecular diversity retained in each subset was not affected by a sampling procedure based upon phenotypic characters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051469
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  • 3
    Electronic Resource
    Electronic Resource
    Application of synteny across Poaceae to determine the map location of a sugarcane rust resistance gene (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 962-969 
    Details
    ISSN: 1432-2242
    Keywords: Key words Sugarcane ; Rust resistance gene ; Comparative mapping ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A major rust resistance gene has been identified in a self-progeny of the sugarcane cultivar R570. Until now, this gene was known to be linked to a marker revealed by the sugarcane probe CDSR29 but unassigned to any linkage group of the current genetic map. We used synteny relationships between sugarcane and three other grasses in an attempt to saturate the region around this rust resistance gene. Comparison of sugarcane, sorghum, maize and rice genetic maps led to the identification of homoeologous chromosome segments at the extremity of sorghum linkage group D, rice linkage group 2, maize linkage group 4 and in the centromeric region of maize linkage group 5. One hundred and eighty-four heterologous probes were selected and tested for cross-hybridization with sugarcane DNA; 106 produced a good hybridization signal and were hybridized on 88 individuals of the R570 selfed progeny. Two hundred and seventeen single-dose markers were added to the R570 genetic map, of which 66% mapped to linkage group VII, together with the rust resistance gene. This gene has now been mapped to the end of a co-segregating group consisting of 19 RFLP markers. None of the mapped loci were located closer to the gene than CDSR29. The gene thus appears to reside at the edge of a ’’synteny cluster’’ used to describe the different grass genomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051568
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  • 4
    Electronic Resource
    Electronic Resource
    Comparative genetic mapping between duplicated segments on maize chromosomes 3 and 8 and homoeologous regions in sorghum and sugarcane (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 1024-1030 
    Details
    ISSN: 1432-2242
    Keywords: Key words Maize ; Sorghum ; Sugarcane ; RFLP ; Synteny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Comparative mapping within maize, sorghum and sugarcane has previously revealed the existence of syntenic regions between the crops. In the present study, mapping on the sorghum genome of a set of probes previously located on the maize and sugarcane maps allow a detailed analysis of the relationship between maize chromosomes 3 and 8 and sorghum and sugarcane homoeologous regions. Of 49 loci revealed by 46 (4 sugarcane and 42 maize) polymorphic probes in sorghum, 42 were linked and were assigned to linkage groups G (28), E (10) and I (4). On the basis of common probes, a complete co-linearity is observed between sorghum linkage group G and the two sugarcane linkage groups II and III. The comparison between the consensus sorghum/sugarcane map (G/II/III) and the maps of maize chromosomes 3 and 8 reveals a series of linkage blocks within which gene orders are conserved. These blocks are interspersed with non-homoeologous regions corresponding to the central part of the two maize chromosomes and have been reshuffled, resulting in several inversions in maize compared to sorghum and sugarcane. The results emphasize the fact that duplication will considerably complicate precise comparative mapping at the whole genome scale between maize and other Poaceae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050225
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  • 5
    Electronic Resource
    Electronic Resource
    RFLP diversity in cultivated sorghum in relation to racial differentiation (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 838-844 
    Details
    ISSN: 1432-2242
    Keywords: Sorghum ; RFLP ; Genetic diversity Cultivated ; Racial differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Careful assessment of the comparative diversity for molecular markers and for potentially-useful morpho-agronomic traits is paramount to the analysis of a genome through the mapping of favorable genes. Sorghum (Sorghum bicolor ssp.bicolor) varieties are traditionally classified into five races on the basis of morphological traits, especially panicle and grain traits. Isozyme diversity has provided a new insight into genetic diversity, and showed a marked geographic structure. We performed RFLP analysis on 94 varieties, chosen to represent the main cross combinations (race × geographic origin), using 35 maize probes that detect polymorphism with at least one of the two restriction enzymesHindIII andXbaI. A total of 50 polymorphic probe-enzyme combinations yielded 158 polymorphic bands. The bicolor race appeared highly variable and included many rare markers. Among the other races multivariate analysis of the data differentiated six clusters corresponding, by decreasing magnitude of divergence, to: the margaritiferum types (a sub-race of race guinea); the guinea forms from western Africa; race caudatum; race durra; race kafir; and the guinea forms from southern Africa.The apparent geographic differentiation was related to the contrasting distribution of these races and to a higher similarity between races localized in southern Africa. The data agree with the current hypotheses on sorghum domestication but reveal associations between neutral markers and traits probably highly subjected to human selection. Whether such associations will be observed with other useful traits, and to what extent they are maintained by genetic linkage, is worth exploring.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01253994
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  • 6
    Electronic Resource
    Electronic Resource
    Construction of a composite sorghum genome map and comparison with sugarcane, a related complex polyploid (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 409-418 
    Details
    ISSN: 1432-2242
    Keywords: Key words Sorghum ; RFLP map ; Comparative mapping ; Sugarcane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A sorghum composite linkage map was constructed with two recombinant inbred line populations using heterologous probes already mapped on maize and sugarcane. This map includes 199 loci revealed by 188 probes and distributed on 13 linkage groups. A comparison based on 84 common probes was performed between the sorghum composite map and a map of a sugarcane (Saccharum spp.) cultivar being developed and presently comprising 10 tentative linkage groups. A straight synteny was observed for 2 pairs of linkage groups; in two cases, 1 sorghum linkage group corresponded to 2 or 3 sugarcane linkage groups, respectively; in two cases 1 sugarcane link- age group corresponded to 2 separate sorghum linkage groups; for 2 sorghum linkage groups, no complete correspondance was found in the sugarcane genome. In most cases loci appeared to be colinear between homoeologous chromosomal segments in sorghum and sugarcane. These results are discussed in relation to published data on sorghum genomic maps, with specific reference to the genetic organization of sugarcane cultivars, and they, illustrate how investigations on relatively simple diploid genomes as sorghum will facilitate the mapping of related polyploid species such as sugarcane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050430
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  • 7
    Electronic Resource
    Electronic Resource
    Comparative genetic mapping between duplicated segments on maize chromosomes 3 and 8 and homoeologous regions in sorghum and sugarcane (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 1024-1030 
    Details
    ISSN: 1432-2242
    Keywords: Maize ; Sorghum ; Sugarcane ; RFLP ; Synteny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Comparative mapping within maize, sorghum and sugarcane has previously revealed the existence of syntenic regions between the crops. In the present study, mapping on the sorghum genome of a set of probes previously located on the maize and sugarcane maps allow a detailed analysis of the relationship between maize chromosomes 3 and 8 and sorghum and sugarcane homoeologous regions. Of 49 loci revealed by 46 (4 sugarcane and 42 maize) polymorphic probes in sorghum, 42 were linked and were assigned to linkage groups G (28), E (10) and I (4). On the basis of common probes, a complete co-linearity is observed between sorghum linkage group G and the two sugarcane linkage groups II and III. The comparison between the consensus sorghum/sugarcane map (G/II/III) and the maps of maize chromosomes 3 and 8 reveals a series of linkage blocks within which gene orders are conserved. These blocks are interspersed with non-homoeologous regions corresponding to the central part of the two maize chromosomes and have been reshuffled, resulting in several inversions in maize compared to sorghum and sugarcane. The results emphasize the fact that duplication will considerably complicate precise comparative mapping at the whole genome scale between maize and other Poaceae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224044
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Towards a saturated sorghum map using RFLP and AFLP markers (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 320-328 
    Details
    ISSN: 1432-2242
    Keywords: Key words Sorghum ; RFLP ; AFLP ; Genetic linkage map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A near-saturated sorghum genetic linkage map was produced using RFLP, AFLP and morphological markers. First a composite, essentially RFLP-based genetic linkage map was obtained from analyses of two recombinant inbred populations. This map includes 343 loci for 11 linkage groups spanning 1352 cM. Since this map was constructed with many previously mapped heterologous probes, it offers a good basis for synteny studies. Separately, an AFLP map was obtained from the analysis of 168 bands revealed from 12 primer pair combinations. It includes 137 loci for 11 linkage groups spanning 849 cM. Taking into account the different data sets, we constructed a combined genetic linkage map including 443 loci spanning 1899 cM. Two main features are to be noted: (1) the distribution of AFLPs along the genome is not uniform; (2) an important stretching of the former core map is induced after adding the AFLPs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051076
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  • 9
    Electronic Resource
    Electronic Resource
    Comparative genome analysis between several tropical grasses (1997)
    Springer
    Euphytica 96 (1997), S. 13-21 
    Details
    ISSN: 1573-5060
    Keywords: genome ; tropical grasses ; comparative mapping ; synteny ; collinearity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The availability of molecular markers allowed comparing genetic maps between distinct taxa. Among the Poaceae (grasses) family, species as divergent as rice, wheat and maize show collinearity within wide regions along the whole genome. This opens the way for the integration of knowledge across all grasses and the development of molecular tools of general interest using the simple genome of rice. Many agronomically important tropical grasses belong to two major sub-families. Comparisons between maize, sorghum and sugarcane, three members of the Andropogoneae tribe, are briefly reviewed. One example of a particular genome region is used for extending the comparison to rice and illustrating various applications of comparative genome mapping.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002987620250
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