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  • 1
    Electronic Resource
    Electronic Resource
    The IgG subclasses of antibodies to grass pollen allergens produced in hay fever patients during hyposensitization (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 6 (1976), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Total IgG, IgG subclass and IgE antibodies specific for grass pollen allergens were measured by the red cell linked antigen-antiglobulin reaction (RCLAAR) in serum samples from nineteen patients who had undergone a course of hyposensitization. Increases in both specific IgG and IgE antibodies were seen after treatment in most patients. In the IgG subclasses the predominant response was for IgG1 and IgG4 antibodies. Attempts were made to correlate the antibody responses with the clinical response and the results are discussed with reference to the possible mechanisms of hyposensitization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1976.tb01901.x
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  • 2
    Electronic Resource
    Electronic Resource
    The IgG subclasses of antibodies to castor bean allergen in patients with allergic asthma: detection of a high incidence of antibodies of the IgG4 subclass (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 5 (1975), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Sera from patients with allergic asthma to castor bean dust were tested for specific antibodies in the red cell linked antigen-antiglobulin reaction (RCLAAR) using antiglobulin sera specific for IgE and the four subclasses of IgG. Approximately one-third of the patients with specific IgG antibodies had an antibody response that was predominantly in the IgG4 subclass. Two patients who were thought not to be sensitive to castor bean allergen, despite long exposure to the dust, did not have detectable IgE antibodies, but had high titres of IgG antibodies that were predominantly IgG4. Specific IgG4 antibodies to castor bean allergen were heat stable, did not appear to passively sensitize monkey skin in the PCA reaction and did not passively sensitize human basophils for the rosette reaction with castor bean-coated red cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1975.tb01874.x
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  • 3
    Electronic Resource
    Electronic Resource
    An RFLP linkage map for loblolly pine based on a three-generation outbred pedigree (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 273-278 
    Details
    ISSN: 1432-2242
    Keywords: RFLP ; Genetic linkage mapping ; Pinus taeda L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genetic linkage map for loblolly pine (Pinus taeda L.) was constructed using segregation data from a three-generation outbred pedigree consisting of four grandparents, two parents, and 95 F2 progeny. The map was based predominantly on restriction fragment length polymorphism (RFLP) loci detected by cDNA probes. Sixty-five cDNA and three genomic DNA probes revealed 90 RFLP loci. Six polymorphic isozyme loci were also scored. One-fourth (24%) of the cDNA probes detected more than 1 segregating locus, an indication that multigene families are common in pines. As many as six alleles were observed at a single segregating locus among grandparents and it was not unusual for the progeny to segregate for three or four alleles per locus. Multipoint linkage analysis placed 73 RFLP and 2 isozyme loci into 20 linkage groups; the remaining 17 RFLP and 4 isozyme loci were unlinked. The mapped RFLP probes provide a new set of codominant markers for genetic analyses in loblolly pine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223631
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  • 4
    Electronic Resource
    Electronic Resource
    Mapped DNA probes from loblolly pine can be used for restriction fragment length polymorphism mapping in other conifers (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 279-282 
    Details
    ISSN: 1432-2242
    Keywords: Pinus taeda L. ; Conifers ; Restriction fragment length polymorphisms (RFLPs) ; Genetic mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A high-density genetic map based on restriction fragment length polymorphisms (RFLPs) is being constructed for loblolly pine (Pinus taeda L.). Consequently, a large number of DNA probes from loblolly pine are potentially available for use in other species. We have used some of these DNA probes to detect RFLPs in 12 conifers and an angiosperm. Thirty complementary DNA and two genomic DNA probes from loblolly pine were hybridized to Southern blots containing DNA from five species of Pinus (P. elliottii, P. lambertiana, P. radiata, P. sylvestris, and P. taeda), one species from each of four other genera of Pinaceae (Abies concolor, Larix laricina, Picea abies, and Pseudotsuga menziesii), one species from each of three other families of Coniferales [Sequoia sempervirens (Taxodiaceae), Torreya californica (Taxaceae) and Calocedrus decurrens (Cupressaceae)], and to one angiosperm species (Populus nigra). Results showed that mapped DNA probes from lobolly pine will cross-hybridize to genomic DNA of other species of Pinus and some other genera of the Pinaceae. Only a small proportion of the probes hybridized to genomic DNA from three other families of the Coniferales and the one angiosperm examined. This study demonstrates that mapped DNA probes from loblolly pine can be used to construct RFLP maps for related species, thus enabling the opportunity for comparative genome mapping in conifers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223632
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  • 5
    Electronic Resource
    Electronic Resource
    A genetic linkage map for Pinus radiata based on RFLP, RAPD, and microsatellite markers (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 673-679 
    Details
    ISSN: 1432-2242
    Keywords: Pinus radiata ; Genetic linkage map ; RFLP ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genetic linkage map for radiata pine (Pinus radiata D. Don) has been constructed using segregation data from a three-generation outbred pedigree. A total of 208 loci were analyzed including 165 restriction fragment length polymorphism (RFLP), 41 random amplified polymorphic DNA (RAPD) and 2 microsatellite markers. The markers were assembled into 22 linkage groups of 2 or more loci and covered a total distance of 1382 cM. Thirteen loci were unlinked to any other marker. Of the RFLP loci that were mapped, 93 were detected by loblolly pine (P. taeda L.) cDNA probes that had been previously mapped or evaluated in that species. The remaining 72 RFLP loci were detected by radiata pine probes from a PstI genomic DNA library. Two hundred and eighty RAPD primers were evaluated, and 41 loci which were segregating in a 1∶1 ratio were mapped. Two microsatellite markers were also placed on the map. This map and the markers derived from it will have wide applicability to genetic studies in P. radiata and other pine species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226088
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  • 6
    Electronic Resource
    Electronic Resource
    Interval mapping of quantitative trait loci affecting NESTUR, a stem growth efficiency index of radiata pine seedlings (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1062-1068 
    Details
    ISSN: 1432-2242
    Keywords: Key words NESTUR ; Stem growth efficiency ; RAPD ; QTL ; Haploid megagametophyte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  NESTUR (needle-to-stem unit rate) is a stem growth index of conifer seedlings that measures the efficiency of stemwood production per unit of needle growth, and is related to other seedling traits such as height, stem diameter, stem volume and needle volume. Quantitative trait loci (QTLs) affecting the expression of stem growth efficiency in radiata pine seedlings were investigated using a RAPD linkage map constructed from markers scored on haploid, megagametophytic DNA. Four putative QTLs were detected which accounted for 8.5–36.4% of the population variance. A search for evidence of epistasis, using both complete pairwise and conditional interactions, did not yield any statistically significant result. Over a 3-year period, seedlings with high-NESTUR marker alleles showed a superior growth performance of 17–40% for height, diameter and volume over those with low-NESTUR marker alleles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050992
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  • 7
    Electronic Resource
    Electronic Resource
    Age-related changes in the expression of QTLs for growth in radiata pine seedlings (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1053-1061 
    Details
    ISSN: 1432-2242
    Keywords: Key words RAPD ; QTL ; Age effects ; Stem growth ; Linkage map ; Haploid megagametophyte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Haploid megagametophytes from a full-sib cross of Pinus radiata were used to construct a genetic linkage map for radiata pine based on random amplified polymorphic DNA (RAPD) markers. The map, which was made from 222 markers, was used to carry out a QTL analysis of growth using measurements made on seedlings from which these megagametophytes were collected at an early germination stage. Trends in the expression of QTLs for stem diameter, volume and height were compared using measurements made at 5 months of age, and at 1, 2 and 3 years of age. None of the observed trends showed complete stability, i.e. none of the putative QTL positions detected at any one age was strongly expressed at all of the four stages of measurement. However, 45% of the trends showed partial stability, i.e. putative QTLs significant at one age were also detected at a subsequent age. Trends in QTL expression with age followed one of three patterns: (1) putative QTLs at some locations showed a gradual linear increase in influence from 5 months of age and were highest at 3 years of age; (2) QTLs detected at 5 months of age gradually became less significant with age; and (3) some putative QTLs showed a curvilinear increase in effect from 5 months of age, reaching their peak expression at 1 to 2 years, and sometimes were still detected at 3 years of age.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050991
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  • 8
    Electronic Resource
    Electronic Resource
    Comparative mapping in loblolly and radiata pine using RFLP and microsatellite markers (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 656-662 
    Details
    ISSN: 1432-2242
    Keywords: Key words Comparative mapping ; RFLP ; Microsatellite markers ; Loblolly pine ; Radiata pine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Genetic linkage maps were constructed for loblolly pine (Pinus taeda L.) and radiata pine (P. radiata D. Don) using a common set of RFLP and microsatellite markers. The map for loblolly pine combined data from two full-sib families and consisted of 20 linkage groups covering 1281 cM. The map for radiata pine had 14 linkage groups and covered 1223 cM. All of the RFLP probes readily hybridise between loblolly and radiata pine often producing similar hybridisation patterns. There were in total 60 homologous RFLP loci mapped in both species which could be used for comparative purposes. A set of 20 microsatellite markers derived from radiata pine were also assayed; however, only 9 amplified and revealed polymorphic loci in both species. Single-locus RFLP and microsatellite markers were used to match up linkage groups and compare order between species. Twelve syntenic groups were obtained each consisting of from 3 to 9 homologous loci. The order of homologous loci was colinear in most cases, suggesting no major chromosomal rearrangements in the evolution of these species. Comparative mapping between loblolly and radiata pine should facilitate genetic research in both species and provide a framework for mapping in other pine species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051281
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  • 9
    Electronic Resource
    Electronic Resource
    Genetic analysis of Verticillium wilt tolerance in cotton using pedigree data from three crosses (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 162-167 
    Details
    ISSN: 1432-2242
    Keywords: Gossypium hirsutum L. ; Verticillium dahliae Kleb. ; Generation means analysis ; Minimum chisquare ; Genetic segregation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three crosses and descendant generations were used in a field study of the inheritance of tolerance to Verticillium wilt, caused by Verticillium dahliae Kleb., in upland cotton (Gossypium hirsutum L.). The tolerant cultivar ‘Acala SJC-1’ was crossed to more susceptible parents, breeding line S5971 and cultivars ‘Acala 4-42’ and ‘Deltapine 70’. Seven generations were evaluated for each cross: the two parents (P1 and P2), F1; F2, F3, and reciprocal backcrosses (B1 and B2). The genetic control of tolerance in these crosses appears to involve more than one gene, based on an unsatisfactory fit to expected phenotypic distributions for the generations under a single-locus model. An analysis of generation means indicated that pooled additive and pooled dominance effects over loci were adequate to explain the variation among generations for crosses of SJC-1 X S5971 and SJC-1 X DPL70. Tolerance in these crosses appeared to be controlled by recessive factors. For the SJC-1 X 4-42 cross, an adequate fit to a digenic epistatic model was not possible, and none of the genetic parameters except the F2 mean were significant. Heritabilities for tolerance to Verticillium wilt, determined from regressions of F3 progeny on F2 parents for the crosses of SJC-1 X S5971 and SJC-1 X DPL70, ranged from 0.12 to 0.28. Therefore, individual plant selection for improved tolerance is expected to be inefficient.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290099
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  • 10
    Electronic Resource
    Electronic Resource
    Inheritance of RFLP loci in a loblolly pine three-generation pedigree (1991)
    Springer
    Theoretical and applied genetics 83 (1991), S. 238-242 
    Details
    ISSN: 1432-2242
    Keywords: Pinus taeda L. ; Restriction fragment length polymorphisms ; genome mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A high-density restriction fragment length polymorphism (RFLP) linkage map is being constructed for loblolly pine (Pinus taeda L.). Loblolly pine cDNA and genomic DNA clones were used as probes in hybridizations to genomic DNAs prepared from grandparents, parents, and progeny of a three-generation outbred pedigree. Approximately 200 probes were evaluated for their ability to detect polymorphic loci between DNAs prepared from the two parent trees, 20–1010 and 11–1060, and cut with four different restriction enzymes: BamHI, DraI, EcoRI, and HindIII. More than half of the probes detecting single- or low-copy number sequences (56%) revealed polymorphisms between the two parents with at least one restriction enzyme. If necessary, an additional hybridization to DNAs prepared from the four grandparent trees was conducted to determine the zygosity of parent trees. Ten of these probes were hybridized to progeny DNAs from this cross and, as expected, the markers were inherited as simple codominant Mendelian alleles. Four of the ten probes detected segregation of three alleles at one locus, and four probes detected more than one independently segregating locus. RFLPs can be used immediately to assess genetic diversity in conifer populations and to “efingerprint” genotypes in tree improvement programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226257
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