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  • 1
    Electronic Resource
    Electronic Resource
    Regulation of acyl CoA: Cholesterol acyl transferase (ACAT) activity by mevalonate and cholesterol in isolated rat hepatocytes during perinatal development (1986)
    Springer
    Bioscience reports 6 (1986), S. 735-740 
    Details
    ISSN: 1573-4935
    Keywords: acyl CoA: cholesterol acyltransferase ; hepatocytes ; cholesterol ; mevalonate ; lipoproteins ; development (rat liver)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Acyl CoA: cholesterol acyl transferase (ACAT) activity presents marked oscillations and differential sensitivity to the “in vitro” stimulation of the kinase-phosphatase modulatory system in the perinatal rat liver. The regulation of this enzyme activity by some modulators generally active in adulthood, such as cholesterol, lipoproteins and mevalonate, has been studied in hepatocytes isolated at different developmental stages. A lack of effect of mevalonate and a positive effort of lipoprotein cholesterol have been observed at the fetal and neonatal stages. A differential prevalence is suggested of one of the two modulatory mechanisms (phosphorylation-dephosphorylation system, or substrate effect) at each developmental stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01116541
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of the asialoglycoprotein receptor in cultured rat hepatocytes is modulated by cell density (1996)
    Springer
    Bioscience reports 16 (1996), S. 477-484 
    Details
    ISSN: 1573-4935
    Keywords: Cultured hepatocytes ; asialoglycoprotein receptor ; cell density dependence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The influence of cell density on expression of the asialoglycoprotein receptor system in primary cultures of rat hepatocytes was evaluated by measuring the level of the receptor specific mRNA. When the hepatocytes are cultured at high cellular density and are not in a proliferative condition, the transcript molecules of the receptor appear increased about 50% with respect to the low plating density, indicating a modulation of asialoglycoprotein receptor expression at transcriptional level. Such control may be dependent on surface molecules involved in cell specific reassociation, since it is well known that cell contacts play a significant regulatory role in differentiated cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01198463
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  • 3
    Electronic Resource
    Electronic Resource
    Some aspects of glycosylation in rat liver during pregnancy (1989)
    Springer
    Bioscience reports 9 (1989), S. 565-572 
    Details
    ISSN: 1573-4935
    Keywords: glycoprotein ; dolichol ; glycosyl transferase ; liver ; pregnancy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The aim of the present report was to analyze the pattern of glycoprotein synthesis in rat liver on 19th and 22nd day of pregnancy by following the incorporation of14C-glucosamine and3H-galactose into isolated rat hepatocytes, the N-acetylglucosaminyl-1-P and galactosyl transferase activities, and the liver content of dolichol and dolichyl-phosphate. The data obtained show a decrease of precursor incorporation into glycoproteins during the last period of pregnancy; this decrease is independent of enzyme activities. The dolichol content increases and the dolichyl-phosphate content, usually considered as rate limiting for glycosylation, decreases. These results, present in other conditions of proliferation and differentiation of rat liver, could explain the differences in membrane organization, the increase of hepatic proteolysis and the alteration in secretory activity during the last phase of gestation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01119799
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  • 4
    Electronic Resource
    Electronic Resource
    Changes in lipoprotein binding and uptake by hepatocytes during rat liver regeneration (1989)
    Springer
    Bioscience reports 9 (1989), S. 231-241 
    Details
    ISSN: 1573-4935
    Keywords: lipoprotein metabolism ; regenerating liver ; apo E receptor ; apo B, E receptor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The binding and uptake of cholesterol enriched lipoproteins by isolated hepatocytes was decreased at 16 hours after partial hepatectomy, with a tendency to return to control values as the regeneration proceeds. The number of lipoprotein binding sites of total cellular membranes remained similar to control at 16 and 24 hours. The plasma lipoprotein pattern, determined by electrophoretic analysis, showed a lower per cent of very low density lipoproteins (VLDL) and a higher per cent of low density lipoproteins (LDL) at 16 and 24 hours post-partial hepatectomy. At these times, plasma lecithin: cholesterol acyltransferase (LCAT) activity was decreased. It is intriguing to suggest that the regenerating liver could regulated the blood lipoprotein pattern and the uptake of lipoproteins by modulating the surface expression of the receptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01116000
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  • 5
    Electronic Resource
    Electronic Resource
    Modulation of asialoglycoprotein binding activity in livers of pregnant or estrogen-treated rats (1989)
    Springer
    Bioscience reports 9 (1989), S. 701-707 
    Details
    ISSN: 1573-4935
    Keywords: asialoglycoprotein ; receptor ; liver ; pregnant rat ; estrogen-treated rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract This report deals with the modulation of activity and expression of the hepatic asialoglycoprotein receptor, in pregnant or diethylstilbestrol-treated rats. The results show a two-fold increase in the total cell associated binding activity, both in pregnant and in estrogen-treated animals, with respect to normal values. On the contrary the surface expression was shown to be strongly enhanced only in the liver of pregnant rat. Therefore the modulation shown by this receptor system in pregnancy seems to be only partially estrogen-dependent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01114808
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  • 6
    Electronic Resource
    Electronic Resource
    Hormonal control of cholesterogenesis and related enzymes in isolated rat hepatocytes during pre- and postnatal development (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 125 (1985), S. 507-511 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effect of glucagon and insulin on the incorporation of 1-14C-acetate into cholesterol and fatty acids and on the enzymes involved in the first steps of cholesterol synthesis (3-hydroxy-3-methyl-glutaryl-coenzyme A reductase, 3-hydroxy-3-methyl-glutaryl-coenzyme A synthase, and acetoacetyl-coenzyme A thiolase) was investigated. Isolated rat hepatocytes at different stages of fetal and postnatal development were employed. Data obtained show the appearance of hormonal control on the 18th day of fetal life, indicating the same pattern, as regards acetate incorporation and HMGCoA reductase prepared and assayed in the presence of NaF. On the contrary, HMGCoA reductase, prepared without NaF, HMGCoA synthase, and acetoacetyl CoA thiolase, does not appear to respond to hormonal stimulation. In the perinatal period, the hormonal effect is no longer detectable, probably because of a hormone resistance of this metabolic pathway.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041250321
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  • 7
    Electronic Resource
    Electronic Resource
    Regulation of amino acid transport in isolated rat hepatocytes during development (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 130 (1987), S. 103-110 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effect of amino acid depletion or supplementation and the effect of glucagon and insulin on the amino acid transport mediated by system A were investigated by determining the uptake of either 2-amino [1-14C]isobutyric acid (AIB) or N-methyl 2-amino [1-14C]isobutyric acid (MeAIB) in rat hepatocytes, freshly isolated at different stages of pre- and postnatal development. The data obtained show that the Na+-dependent uptake was higher at the earliest developmental stages, and steadily decreased until the adult level. The hormones increased AIB and MeAIB uptake enhancing the Vmax, while the Km was unchanged. This effect was evident in cells from adult and 18-20-day-old fetuses, while no response was present before the 18th day of fetal life and in the perinatal period. Actinomycin D or cycloheximide abolished this hormone-dependent increase. A decrease in AIB and MeAIB transport after incubation in an amino acid-rich medium was demonstrated at all ages tested, but was particularly evident in the prenatal life. The increase in the activity of the system following amino acid starvation was shown to be mostly dependent from de novo protein synthesis in the fetal life; on the contrary in the adult the increase appeared to be more linked to the releas from transinhibition of the transport.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041300115
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  • 8
    Electronic Resource
    Electronic Resource
    Different signal transduction by epidermal growth factor may be responsible for the difference in modulation of amino acid transport between fetal and adult hepatocytes (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 155 (1993), S. 549-555 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: [1-14C]-2-aminoisobutyric acid (AIB) uptake and signal transduction pattern after epidermal growth factor (EGF) stimulation were examined in freshly isolated hepatocytes from 20-day-old fetuses and 3-month-old rats. EGF induced a transient increase of AIB transport after 10 min only in adult animals; the observed unresponsiveness of fetal liver is not dependent on a lack of EGF receptors which are present though to a lesser extent on the plasma membrane in this period. As far as the production of the second messengers, inositol trisphosphate (IP3) and calcium, is concerned, substantial differences were found: EGF increased IP3 production in adult hepatocytes, whereas it had no effect in fetal ones. Moreover, the addition of EGF induced a calcium transient in hepatocytes from adult animals, while there was no increase in fetal cells. The lack of EGF effect on amino acid transport in fetal cells could be due to its inability to produce both IP3 and calcium transients, suggesting that this transduction pathway is not activated during fetal life.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041550313
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