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  • 1
    Digitale Medien
    Digitale Medien
    Beyond 2000: Chemicals from biotechnology — ecological challenge and economic restraints (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 16 (1995), S. 0 
    Details
    ISSN: 1574-6976
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6976.1995.tb00157.x
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  • 2
    Digitale Medien
    Digitale Medien
    Isolation and some properties of lysineN 6-hydroxylase fromEscherichia coli strain EN222 (1989)
    Springer
    BioMetals 2 (1989), S. 1-5 
    Details
    ISSN: 1572-8773
    Schlagwort(e): LysineN 6-hydroxylase ; External flavoprotein (FAD) monooxygenase ; Aerobactin ; Escherichia coli
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary Lysine N6-hydroxylase was isolated as a soluble enzyme from the supernatant after ultrasonication ofEscherichia coli strain EN222 which contained the structural gene on a multicopy plasmid (as described by Engelbrecht and Braun in 1986). The apoenzyme prepared by dialysis was purified by ammonium sulfate precipitation and fast protein liquid chromatography using Superose 12 and Mono Q columns. The molecular mass as determined by gel filtration was 200 kDa and 50 kDa by SDS/polyacrylamide gel electrophoresis. The enzyme binds 0.79 molecule FAD/50 kDa. The activity of the enzyme is strictly dependent on NADPH. Its properties are similar to other flavoprotein monooxygenases of the EC group 1.14.13.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01116193
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  • 3
    Digitale Medien
    Digitale Medien
    Population dynamics of biofilm development during start-up of a butyrate-degrading fluidized-bed reactor (1991)
    Springer
    Applied microbiology and biotechnology 36 (1991), S. 404-409 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Population dynamics during start-up of a fluidized-bed reactor with butyrate or butyrate plus acetate as sole substrates as well as biofilm development on the sand substratum were studied microbiologically, immunologically and by scanning electron microscopy. An adapted syntrophic consortium consisting of Syntrophospora sp., Methanothrix soehngenii, Methanosarcina mazei and Methanobrevibacter arboriphilus or Methanogenium sp. achieved high-rate butyrate degradation to methane and carbon dioxide. Desulfovibrio sp., Methanocorpusculum sp., and Methanobacterium sp. were also present in lower numbers. Immunological analysis demonstrated methanogens antigenically related to Methanobrevibacter ruminantium M1, Methanosarcina mazei S6, M. thermophila TM1, Methanobrevibacter arboriphilus AZ and Methanothrix soehngenii Opfikon in the biofilm. Immunological analysis also showed that the organisms isolated from the butyrate-degrading culture used as a source of inoculum were related to M. soehngenii Opfikon, Methanobacterium formicicum MF and Methanospirillum hungatei JF1.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00208165
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  • 4
    Digitale Medien
    Digitale Medien
    Start-up and operation of a propionate-degrading fluidized-bed reactor (1992)
    Springer
    Applied microbiology and biotechnology 36 (1992), S. 810-816 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary A laboratory-scale fluidized-bed reactor was inoculated with a syntrophically propionate-degrading co-culture. After 24 days of batch operation with propionate and acetate in the medium, the reactor was operated for 8 months with propionate as the sole substrate. The loading rate was 8.5 kg chemical oxygen demand/m3 ·day, yielding a maximal gas production of 4.5 l/l·day at a removal efficiency of 94–99%. The degradation was not inhibited by up to 85mm propionate in pulse experiments, but short-time changes in redox potential above — 300 mV led to a drop in the propionate degradation rate. While Methanocorpusculum sp. and Methanospirillum sp. adhered to the sand in the early phase of the start-up, the consortium in the mature biofilm consisted of Desulfobulbus sp., Methanothrix soehngenii and species of at least three different genera of hydrogenotrophic methanogens. A syntrophic relationship between the sulphate-reducing Desulfobulbus sp. and hydrogenotrophic and acetotrophic methanogens is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00172200
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  • 5
    Digitale Medien
    Digitale Medien
    A model for penicillin production with and without temperature shift after the growth phase (1992)
    Springer
    Applied microbiology and biotechnology 36 (1992), S. 446-451 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary A strain of Penicillium chrysogenum producting about 8 g/l of penicillin V, was cultivated in a 10-1 bioreactor. Under carbon (C)-limitation during the production phase a glucose/ammonium sulphate mixture was fed using microprocessor control. When the temperature was shifted from 25° C to 30° C at the end of the active growth phase, the specific penicillin production rate was increased by 30%, while the yield remained constant. Maximal productivity without sporulation was obtained when the net growth rate of the active (respiring and producing) biomass, estimated by measuring the respiration rate under defined conditions, was equal to or higher than 0.004 h−1. A model was developed for penicillin fermentation during C-limitation possessing the following properties: (1) the model is based on ordinary differential equations; (2) the influence of different nutrients is considered; (3) the model recognizes two cell types (active and inactive); (4) the model describes the influence of a temperature shift at the end of the vigorous growth phase.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00170180
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  • 6
    Digitale Medien
    Digitale Medien
    Enrichment of acetate-, propionate-, and butyrate-degrading co-cultures from the biofilm of an anaerobic fluidized bed reactor (1989)
    Springer
    Applied microbiology and biotechnology 31 (1989), S. 425-429 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Scanning electron microphotographs from the biofilm of a pilot scale anaerobic fluid-ized-bed reactor fed with acetate, propionate, and butyrate as carbon sources showed a predominance of filamentous organisms resembling Methanothrix sp. which could be isolated as an al-most pure culture as well as a Methanosarcina strain. Three syntrophic cultures, enriched in the medium of Boone and Xun, contained four or five microscopically distinguishable microorganisms, among them Methanospirillum sp., Methanothrix sp., Methanosarcina sp., and rods of acetogenic bacteria degrading propionate or butyrate effectively.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00257616
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  • 7
    Digitale Medien
    Digitale Medien
    Degradation of dioxane, tetrahydrofuran and other cyclic ethers by an environmental Rhodococcus strain (1991)
    Springer
    Applied microbiology and biotechnology 36 (1991), S. 120-123 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary By enrichment and isolation techniques bacterial strains with the capacity to grow on aliphatic cyclic ethers (dioxane, tetrahydrofuran, 1,3-dioxolane) have been isolated. Six strains that degrade tetrahydrofuran were classified as belonging to the genus Rhodococcus. One of two strains that degrade dioxane instead of or in combination with tetrahydrofuran was further characterized and a hypothetical catabolic pathway comprising an initial 2-hydroxylation and several oxidation steps is postulated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00164711
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  • 8
    Digitale Medien
    Digitale Medien
    Estimation of fermentation biomass concentration by measuring oxygen uptake off-line with an oxygen electrode (1989)
    Springer
    Applied microbiology and biotechnology 32 (1989), S. 32-36 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary An indirect method for biomass determination was developed for aerobic cultivation in media containing solid substrates. This method involved off-line estimation of oxygen uptake with an oxygen electrode using a calibration factor and was applied to penicillin fermentation with Penicillium chrysogenum. The volumetric oxygen uptake rate was independent of the amount of sample sealed in the measuring cell. Storage of the sample for a period up to 2.5 h also had no influence on the rate of O2 uptake. The specific O2 uptake rate at 70 to 75 h fermentation time was used as the “calibration factor” to calculate the respiration-active part of the biomass. Specific penicillin V formation rates related to net dry weight, respiration-active biomass (X A )and RNA content were compared. Only the penicillin V production rate related to X A showed a similar behaviour as the penicillin V production rate related to RNA content. For this reason, it is assumed that X A ,like RNA content, is more closely related to the metabolic state of the cells than the net dry weight. This method, therefore, provides a more suitable reference parameter for fermentation control than the generally used net dry weight.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00164819
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  • 9
    Digitale Medien
    Digitale Medien
    Parameters of unbalanced growth and reversible inhibition of deoxyribonucleic acid synthesis in Brevibacterium ammoniagenes ATCC 6872 induced by depletion of Mn2+. Inhibitor studies on the reversibility of deoxyribonucleic acid synthesis (1980)
    Springer
    Archives of microbiology 127 (1980), S. 105-114 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Manganese deficiency ; Unbalanced growth ; Brevibacterium ammoniagenes ; Reversible inhibition of DNA synthesis ; Inhibition by hydroxyurea ; Mn2+-Controlled ribonucleotide reduction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Unbalanced growth induced by depletion of manganese ions was a prerequisite for production of ribonucleotides in a high salt mineral medium with the wildtype strain Brevibacterium ammoniagenes ATCC 6872. The concentration of manganese strictly controlled the overall deoxyribonucleic acid (DNA) synthesis, whereas ribonucleic acid (RNA), protein and cell wall synthesis remained essentially unimpaired in the manganese-lacking cells. The reversibility of inhibition of overall DNA synthesis was shown by enhanced incorporation (up to threefold compared to the cultures supplied with sufficient manganese) of [8-14C] adenine into alkali-stable, trichloroacetic acid-insoluble material after subsequent addition of 10 μM MnCl2 to 15 h-old depleted cultures. The results of inhibitor studies on the restoration of overall DNA synthesis due to subsequent addition of manganese ions to depleted cultures suggest that ribonucleotide reduction is the primary target of the manganese starvation during nucleotide fermentation with Brevibacterium ammoniagenes ATCC 6872.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00428013
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  • 10
    Digitale Medien
    Digitale Medien
    Die Mikroflora eines Langzeit-Sauerteiges (1992)
    Springer
    European food research and technology 194 (1992), S. 536-540 
    Details
    ISSN: 1438-2385
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die Milchsäurebakterien und Hefen eines kommerziellen Sauerteiges wurden isoliert und identifiziert. Dieser Sauerteig wird erhalten, wenn Roggenmehl und Wasser für mindestens 48 h gesäuert und wöchentlich in neues unsteriles Substrat überimpft wird. Alle gefundenen Milchsäurebakterien waren heterofermentativ, 2/3 von ihnen hatten ein enges Substratspektrum, bildeten Ammoniak aus Arginin, waren Acetat-auxotroph und produziertenl-Lactat und Ethanol. Eine kleinere Gruppe vergor mehr als 15 (von 49 getesteten) Zuckern, bildete kein Ammoniak aus Arginin und produziertedl-Lactat. Alle untersuchten Stämme enthielten in der Zellwand Lysin-d-Asparagin als Interpeptid-Brücke des Peptidoglykans. Alle Hefe-Isolate ergaben gleiche Resultate im Zucker-Assimilationstest und waren Cycloheximid-empfindlich. Der Hefestamm wurde alsSaccharomyces cerevisiae Hansen identifiziert.
    Notizen: Summary The lactic acid bacteria and yeasts of a commercial sourdough were isolated and identified. This sourdough is obtained when rye flour and water are fermented for at least 48 h with weekly transfer to new unsterile substrate. All of the bacteria were heterofermentative, two-thirds of them had a narrow substrate spectrum, formed ammonia from arginine, were acetate-auxotrophic and producedl-lactate and ethanol. A minor group fermented more than 15 sugars (of 49 tested), were unable to form ammonia from arginine and produceddl-lactate. Of the strains tested all contained lysine-D-asparagine as an interpeptide bridge in the peptidoglycan of the cell wall. Each of the yeast isolates gave identical results in the sugar assimilation test and was cycloheximide sensitive. The yeast strain was identified asSaccharomyces cerevisiae Hansen.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01185479
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