ISSN:
1432-203X
Keywords:
Abbreviations: BA, 6-benzylaminopurine; BMP, basal medium propagation; BMR, basal medium rooting; DAPI, 4', 6-diamino-2-phenylindole; KIBA, potassium salt of indole-3-butyric acid; kinetin, 6-furfurylaminopurine; MS, Murashige and Skoog; NAA, 1-naphthaleneacetic acid
;
Key words:Primulaceae, tissue culture, regeneration, somaclonal variation, organogenesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary. In long-term callus cultures of Cyclamen persicum Mill. two types of tissue could be distinguished. One type featured a brown suberised outer layer and was poorly organogenic. The other type was yellowish in appearance and gave rise to many shoot buds. Both types co-existed on the same callus, the former prevailing. Selection for organogenic tissue resulted in cultures yielding approximately three times more petioles than random subcultures. Callus-derived shoots could be rooted and established in the greenhouse. The method allowed for the production of thousands of plants but the regenerants often showed deviant phenotypes and genotypes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050071
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