ISSN:
1573-6857
Keywords:
Ac/Ds
;
transformation
;
transgenic plants
;
transposon tagging
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have recently shown that a plasmid-borneDissociation (Ds) element can excise from extrachromosomal plasmid DNA and integrate into a plant genome in the presence of theActivator (Ac) transposase.Ds andAc-carrying plasmids were used to co-transformNicotiana plumbaginifolia protoplasts. Transgenic plants were regenerated and analyzed. Here we describe further characterization of the system and discuss its efficiency in terms of DNA transformation and transposon tagging.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01435238
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