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  • 1
    Digitale Medien
    Digitale Medien
    CYTODEUTEROPORPHYRIN1 (1959)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 81 (1959), S. 250-250 
    Details
    ISSN: 1520-5126
    Quelle: ACS Legacy Archives
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1021/ja01510a063
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  • 2
    Digitale Medien
    Digitale Medien
    Growth and anthocyanin accumulation in carrot cell suspension cultures growing on fructose, glucose, or their mixtures (1991)
    s.l. : American Chemical Society
    Biotechnology progress 7 (1991), S. 288-290 
    Details
    ISSN: 1520-6033
    Quelle: ACS Legacy Archives
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1021/bp00009a014
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  • 3
    Digitale Medien
    Digitale Medien
    Cytodeuteroporphyrin1,2 (1960)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 82 (1960), S. 3183-3188 
    Details
    ISSN: 1520-5126
    Quelle: ACS Legacy Archives
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1021/ja01497a049
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  • 4
    Digitale Medien
    Digitale Medien
    The Effect of Carbohydrate and Nitrogen Concentration on Phenol Synthesis in Paul's Scarlet Rose Cells Grown in Tissue Culture (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 39 (1977), S. 0 
    Details
    ISSN: 1399-3054
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: The effects of exogenous concentrations of glucose and nitrate on total ethanol extractable phenols and leucoanthocyanins were studied in Paul's Scarlet Rose cells grown in either liquid suspension or solid culture. Aliquots of liquid suspension cultured cells were harvested during logarithmic, early stationary, and late stationary periods of growth for determination of fresh weight, dry weight, total ethanol extractable phenols and leucoanthocyanins. Cells produced phenols during all phases of growth, but at stationary phase, the production was greatest. Increasing concentrations of exogenous glucose in the culture medium resulted in an increased synthesis of total phenols in logarithmic cells, and an increased synthesis of total phenols and leucoanthocyanin in stationary cells. Addition of increased concentrations of exogenous nitrate to the stationary cells grown in suspension culture markedly reduced synthesis of leucoanthocyanins although total phenol synthesis was not significantly affected. Similar observations were made in cells cultured on solid medium in respect to exogenous glucose concentration, however these cells differed from the suspension cultured cells by having increased amounts of total phenol synthesis and decreased synthesis of leucoanthocyanins in the presence of increasing concentrations of exogenous nitrate in the culture medium.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1399-3054.1977.tb09291.x
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  • 5
    Digitale Medien
    Digitale Medien
    Sources of Nitrogen Supporting Growth and Embryogenesis in Cultured Wild Carrot Tissue (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 37 (1976), S. 0 
    Details
    ISSN: 1399-3054
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: This paper seeks to calarify conflicting reports on the nitrogen requirements for in vitro embryogenesis in Daucus carota.Tissue derived from petiole explants of the wild strain of this species were tested with a variety of sources of cellular nitrogen under conditions otherwise favorable for in vitro embryogenesis. The use of very small, sieved and well-washed inocula reduced the carry-over of soluble materials with the inoculum. Embryo yield was quantified by direct counting of samples.Nitrate at concentrations ranging from 5 to 95 mM KNO3 supportes only weak growth and very low embryogenesis under the exacting conditions of these experiments. As little as 0.1 mM NH4Cl added to a nitrate medium allows some embryogenesis and 10 mM NH4Cl is near optimal when KNO3 is in the range of 12 to 40 mM concentration. Glutamine, glutamic acid, urea and alanine can individually partially replace NH4Cl as a supplement to KNO3. Glutamine, alanine, and possibly glutamic acid can serve as sole sources of nitrogen supporting both good growth and embryogenesis. It was concluded that a reduced nitrogen source is required, at least as a supplement to nitrate, for rapid growth and for in vitro embryogenesis of cultured wild carrot tissue. The relationship of pH of the culture medium to growth and embryogenesis was explored and optima observed at approximately pH 5.4 for both processes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1399-3054.1976.tb03939.x
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  • 6
    Digitale Medien
    Digitale Medien
    Isopropyl m-Chlorocarbanilate and Its Hydroxylated Metabolites: Their Effects on Cell Suspensions and Cell Divison in Soybean and Carrot (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 40 (1977), S. 0 
    Details
    ISSN: 1399-3054
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Hydroxylated metabolites of isopropyl m-chlorocarbanilate (chlorpropham) are found in intact soybean plants (Glycine max Merr.). The metabolites are isopropyl 2-hydroxy-5-chloro-carbanilate (2OH) and isopropyl 3-chloro-4-hydroxycarbanilate (4OH). The phytotoxicity of these metabolites and chlorpropham was tested in cell suspensions and roots of intact soybean seedlings and cell cultures of carrot (Daucus carota L.). The growth of soybean cell suspensions was inhibited with 50 μM chlorpropham. Ten μM chlorpropham usually slowed initial growth of the cultures while 5 μM and 0.1 μM chlorpropham had no effect. The 2OH and 4OH metabolites had no significant effect on dry weight over the same concentration range. Some metabolism of chlorpropham, 2OH and 4OH occurred during 6 and 48 h of incubation with soybean cells. The results are interpreted to mean that all three analogs penetrated into the cells, were metabolized, and some of the metabolites excreted back into the medium. Mitotic index studies of intact 3-day-old soybean roots showed that 2OH inhibited mitosis to a greater extent than chlorpropham, whereas 4OH produced only a slight and insignificant reduction compared to controls. Chlorpropham, 2OH and 4OH (at 50 μM) all reduced the growth of wild carrot cultures grown in the presence or absence of 2,4-D. Therefore, hydroxylation of chlorpropham at the 2′ or 4′ positions of the 5′ chlorinated benzene ring is not sufficient to render the compound nonphytotoxic in all plant systems.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1399-3054.1977.tb01485.x
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  • 7
    Digitale Medien
    Digitale Medien
    Dihydroquercetin accumulation in the medium of some wild carrot culture subclones (1989)
    Springer
    Plant cell, tissue and organ culture 18 (1989), S. 105-112 
    Details
    ISSN: 1573-5044
    Schlagwort(e): biosynthetic intermediate ; anthocyanin biosynthesis ; clonal differences ; mass spectrometry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Approximately half of the subclones examined from one clone of the wild carrot cell culture WC63-1-9-1 accumulated dihydroquercetin in the culture medium. The amount of dihydroquercetin accumulated in the medium varied with the subclone used, the size of the inoculum, the medium used and the time of sampling.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00033469
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  • 8
    Digitale Medien
    Digitale Medien
    Nutrient utilization during biomass and anthocyanin accumulation in suspension cultures of wild carrot cells (1989)
    Springer
    Plant cell, tissue and organ culture 18 (1989), S. 95-104 
    Details
    ISSN: 1573-5044
    Schlagwort(e): ammonia ; succinate ; phosphate ; sucrose hydrolysis ; fructose utilization ; pH
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The medium used for the growth of anthocyanin-accumulating wild carrot (D. carota) suspension cultures contained ammonia as a sole nitrogen source and was buffered with succinate. Ammonia was the first nutrient to be completely utilized. The uptake of carbohydrate, phosphate and succinate continued after ammonia depletion. Biomass accumulation was faster and greater when sucrose was initially present in the medium than when glucose was present. When sucrose was provided in the medium it was rapidly hydrolysed to glucose and fructose and the fructose was used preferentially to glucose. Anthocyanin accumulation was rapid after ammonia fell below 3 mM and until the pH of the medium rose from 4.5 to 5.1 or 5.2.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00033468
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  • 9
    Digitale Medien
    Digitale Medien
    Phenotypic differences in anthocyanin accumulation among clonally related cultured cells of carrot (1990)
    Springer
    Plant cell, tissue and organ culture 22 (1990), S. 213-222 
    Details
    ISSN: 1573-5044
    Schlagwort(e): anthocyanin accumulation ; cell clones ; chalcone synthase ; Daucus carota ; intermediates in biosynthesis ; phenotypes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Subclones from a wild carrot cell culture have been examined for their anthocyanin accumulation in the absence and presence of DMSO and 4-coumaric acid, naringenin, dihydroquercetin or leucocyanidin. Subclones that accumulate no or extremely low levels of anthocyanin do not increase their anthocyanin accumulation when treated with DMSO or intermediates. These compounds increased the anthocyanin accumulation in subclones which produce detectable anthocyanin in their absence. Chalcone synthase was shown to be present in clones and the activity showed no correlation with the amount of anthocyanin accumulated. This suggests that the enzymes of anthocyanin biosynthesis are not coordinately repressed in the subclones which accumulate little or no anthocyanin. Dihydroquercetin and catechin were present in subclones with little or no anthocyanin but no procyanidin was detected which suggests that these subclones biosynthesize leucocyanidin but do not convert it into colorless procyanidins as a major alternative metabolic pathway to anthocyanin biosynthesis. The possibility that some clones are not anthocyanin accumulating because they have impaired transport of the sinapoylated anthocyanin into the vacuole is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00033639
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  • 10
    Digitale Medien
    Digitale Medien
    Anthocyanin yields of clonal wild carrot cell cultures (1990)
    Springer
    Plant cell, tissue and organ culture 23 (1990), S. 79-91 
    Details
    ISSN: 1573-5044
    Schlagwort(e): anthocyanin production ; cell cloning ; Daucus carota ; selection ; suspension cultures ; yield variability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The anthocyanin yields in clonal populations of wild carrot suspension cultures were measured after four patterns of cloning and selection. These patterns were: 1. serial selection of high yielding clones, 2 and 3. one selection of a low yielding clone followed by serial selection of high yielding clones, 4. serial selection of low yielding clones. In all cases the populations of clones obtained showed a wide range of anthocyanin accumulation. The population means of anthocyanin content also varied on serial cloning plus selection for high yield. One selection for low yield substantially decreased the anthocyanin accumulation in the populations obtained by subsequent serial cloning plus selection for high yield in about half the cases. Serial cloning plus selection for low yield decreased the anthocyanin accumulation to low and possibly zero levels in 3 out of 4 cases. The anthocyanin accumulation of these populations could be partially restored by serial recloning plus selection of the highest yielding clones. In one low yielding clonal population a marked increase in anthocyanin occurred during serial passaging. The data lead to the conclusion that the cell from which a clonal population is initiated affects the means of the clonal populations subsequently obtained from it. The implications of this hypothesis are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00035827
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