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  • 1
    Electronic Resource
    Electronic Resource
    Chloroplast protein topogenesis: import, sorting and assembly
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Reviews on Biomembranes 1071 (1991), S. 221-253 
    Details
    ISSN: 0304-4157
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0304-4157(91)90015-O
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Reconstitution of mature plastocyanin from precursor apo-plastocyanin expressed in Escherichia coli
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Bioenergetics 1058 (1991), S. 107-112 
    Details
    ISSN: 0005-2728
    Keywords: Electron transport ; Photosynthesis ; Plastocyanin ; Precursor plastocyanin ; [abr] DTT; dithiothreitol ; [abr] IPTG; isopropyl 1-thio-β-d-galactopyranoside ; [abr] P700; Photosystem I reaction center chlorophyll ; [abr] PC; plastocyanin ; [abr] PMSF; phenylmethylsulfonyl fluoride ; [abr] PS I; Photosystem I
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0005-2728(05)80226-9
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of defender against apoptotic death (DAD-1) in Iris and Dianthus petals (2003)
    Oxford, UK : Munksgaard International Publishers
    Physiologia plantarum 117 (2003), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The gene defender against apoptotic death (DAD-1) prevents programmed cell death in animal cells. We investigated the expression pattern of DAD-1 in petals of iris (Iris × hollandica cv. Blue Magic) and carnation (Dianthus caryophyllus cv. Etarro). DAD-1 expression in Iris petals was strongly reduced by the time of visible senescence, which occurs 4 days after flower opening. Microscopic analysis showed that most mesophyll cells had died prior to a clear decrease in DAD-1 expression and that epidermis cells started to die by that time. In carnation petals DAD-1 expression also decreased by the time of massive cell death. After ethylene treatment, DAD-1 expression in carnation again decreased concomitant with the advance in massive cell death. In conclusion, DAD-1 is not an early regulator of petal cell death. Its expression may be required for the programmed dismantling of cells, as it ceases only just prior to, or concomitant with, cell death.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.2003.1170213.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Enhanced expression in tobacco of the gene encoding green fluorescent protein by modification of its codon usage (1997)
    Springer
    Plant molecular biology 33 (1997), S. 989-999 
    Details
    ISSN: 1573-5028
    Keywords: transgenic ; GFP ; synthetic gene ; codon usage ; cryptic intron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gene encoding green fluorescent protein (GFP) from Aequorea victoria was resynthesized to adapt its codon usage for expression in plants by increasing the frequency of codons with a C or a G in the third position from 32 to 60%. The strategy for constructing the synthetic gfp gene was based on the overlap extension PCR method using 12 long oligonucleotides as the starting material and as primers. The new gene contains 101 silent nucleotide changes compared to its wild-type counterpart used in this study. Several transgenic tobacco lines containing the wild-type gfp gene contained minute amounts of a smaller protein cross-reacting with GFP antiserum, whereas only one protein of the expected size was found in transgenics with the synthetic gfp gene. The smaller protein was probably encoded by a truncated gfp mRNA created by splicing of a 84 bp cryptic intron as detected by a reverse transcription-PCR technique. A comparison of GFP production in transgenics with the wild-type and the synthetic gfp gene under the control of the enhanced CaMV 35S promoter showed that the large-scale alterations in the gfp gene increased the frequency of high expressors in the transgenic population but hardly changed the maximum GFP concentrations.The latter phenomenon may be attributed to a reduced regeneration capacity of transformed cells with higher GFP concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005740823703
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    Paper (German National Licenses)
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