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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 51 (1988), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The lateral hypothalamus has an important role in regulating food and water intake. We have investigated the endogenous release of monoamines from the lateral hypothalamus during manipulations of plasma osmolality and circulating volume. Adult male Sprague-Dawley rats implanted with carbon paste in vivo electrochemical (EC) electrodes in the lateral hypothalamus were placed on a 72-h water deprivation schedule. Although the carbon paste EC electrode has an intrinsically ambiguous signal in which changes in ascorbic acid may appear as changes in catechol concentrations, pharmacologic studies in lateral hypothalamus indicated that the electrode most likely measured nor-epinephrine and possibly epinephrine. On the test day, the EC electrodes were scanned with linear sweep voltammetry from -0.2 to +0.4 V at a rate of 5 mV/s. Semiderivative signal processing showed catechol and hydroxyindole peaks at +0.11 and +0.23 V, respectively. Baseline recordings were made prior to rats drinking distilled water, 10% sucrose, 5% dextrose, 0.30% NaCl, 0.90% NaCl, or 10%D-mannitol. To control for the act of drinking, other implanted dehydrated rats were intraperitoneally injected with 5% dextrose, 0.30% NaCl, or 0.90% NaCl. To dissociate the effects of osmolality and circulating volume on the EC response, hydrated rats implanted with EC electrodes were subcutaneously injected with 12% NaCl or intraperitoneally injected with 35% polyethylene glycol. Other rats subjected to water deprivation and osmotic challenges were decapitated and trunk blood was collected for measurements of plasma osmolality and hematocrit. Similar experiments were conducted using homozygous Brattleboro rats which lack arginine vasopressin (AVP) but which preserve normal plasma osmolality with prodigious drinking. The use of these rats provided a way to dissociate monoamine changes related to AVP release from changes related to plasma osmolality. Results in Sprague-Dawley and Brattleboro rats showed that the EC catechol signal was lower in rats with high plasma osmolality and rose with drinking or intraperitoneal fluid administration as a direct function of the fall in plasma osmolality. Manipulations of plasma osmolality had a greater effect on the EC catechol signal than did manipulations of circulating volume. Thus, we conclude that changes in extracellular fluid catechol concentrations in the lateral hypothalamus linearly reflect changes in plasma osmolality.
    Type of Medium: Electronic Resource
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  • 2
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    Unknown
    Berlin : Periodicals Archive Online (PAO)
    Orientalistische Literaturzeitung. 49 (1954) 76 
    ISSN: 0030-5383
    Topics: Linguistics and Literary Studies , Ethnic Sciences , History
    Notes: Besprechungen
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