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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Analytical chemistry 54 (1982), S. 671-674 
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Analytical chemistry 51 (1979), S. 1487-1492 
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1600-0501
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Titanium platelets with a sand-blasted and acid-etched surface were coated with bovine serum albumin and incubated with a suspension of Porphyromonas gingivalis (ATCC 33277). Four groups with a total of 48 specimens were formed. Laser irradiation of the specimens (n=12) was performed on a computer-controlled XY translation stage at pulse energy 60 mJ and frequency 10 pps. Twelve specimens were treated with an air powder system. After the respective treatment, human gingival fibroblasts were incubated on the specimens. The proliferation rate was determined by means of fluorescence activity of a redox indicator (Alamar Blue® Assay) which is reduced by metabolic activity related to cellular growth. Proliferation was determined up to 72 h. Contaminated and non-treated as well as sterile specimens served as positive and negative controls. Proliferation activity was significantly (Mann–Whitney U-test, P〈0.05) reduced on contaminated and non-treated platelets when compared to sterile specimens. Both on laser as well as air powder-treated specimens, cell growth was not significantly different from that on sterile specimens. Air powder treatment led to microscopically visible alterations of the implant surface whereas laser-treated surfaces remained unchanged. Both air powder and Er : YAG laser irradiation have a good potential to remove cytotoxic bacterial components from implant surfaces. At the irradiation parameters investigated, the Er : YAG laser ensures a reliable decontamination of implants in vitro without altering surface morphology.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of fluorescence 4 (1994), S. 45-48 
    ISSN: 1573-4994
    Keywords: pH measurement on surfaces ; immobilization ; fluorescence spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The attachment of pH-sensitive Fluorescent dyes on to hydroxylapatite for measuring the pH of small volumes is described. Fluorescein and acridine were used, both activated with reactive functions, such as isothiocyanate or succinimidyl ester groups, to enhance the possibility of a covalent linkage to the surface. First investigations were carried out on synthetic hydroxylapatite as the surface material. After the preparation of a particular surface with the fluorescent dye, steady-state and time-resolved fluorescence spectroscopies were employed for estimating the pH value of a solution applied to the surface. In this paper we present the results of our investigations done with both methods. Fluorescein shows significant variation in excitation spectra with pH, whereas in the case of acridine, the fluorescence lifetimes are very sensitive to pH.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1615-6714
    Keywords: Caries ; Confocal laser scanning microscopy ; Glass-lonomer ; Microradiography ; Orthodontic patients ; Karies ; Konfokale Laserrastermikroskopie ; Glasionomer ; Mikroradiographie ; Kieferorthopädische Patienten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Ziel der vorliegenden Studie war es, das kariostatische Potential des kunststoffmodifizierten Glasionomers (Vitremer® Stumpfaufbaufüllung, 3M Dental Product Division) bei Verwendung als Kleber für kieferorthopädische Brackets zu untersuchen. Mit quantitativer Mikroradio-graphie (TMR) und konfokaler Laserrastermikroskopie (CLSM) wurden die Mineraldichte und-verteilung in der Schmelzoberfläche nahe dem Bracketsockel gemessen. Die Studie wurde mit neun Paaren von Prämolaren, die zur Extraktion vorgesehen waren, in der “Split-Mouth”-Technik durchgeführt. Ein Zahn jedes Paares wurde mit dem kunst-stoffmodifizierten Glasionomerbracketkleber behandelt, der als Kontrolle dienende kontralaterale Prämolar mit einem nichtfluoridierten Komposit (Concise®, 3M Dental Product Devision). Jeweils beide Zähne wurden nach vier Wochen extrahiert und bis zu den analytischen Untersuchungen aufbewahrt. Die Läsionstiefen und die Werte für den Mineralverlust in Schmelzbereichen nahe den Brackets waren bei den mit Vitremer®-Bracketkleber behandelten Zähnen signifikant niederer als bei den mit Kompositbracketkleber behandelten. Dies ist ein Hinweis darauf, daß das kunststoffmodifizierte Glasionomer unter In-vivo-Bedingungen die Entwicklung kariöser Läsionen signifikant reduzierte. CLSM-Bilder von den Bereichen um die Brackets zeigen deutliche kariöse Veränderungen und bestätigen damit die TMR-Messungen. Die kombinierte Anwendung von quantitativer TMR und von CLSM bietet besondere Möglichkeiten zur Untersuchung von kariös angegriffenem Hartgewebe. Die fluoridfreisetzenden Eigenschaften des kunststoffmodifizierten Glasionomers sind eine wertvolle Fluoridierungsergänzung bei kieferorthopädischen Behandlungen.
    Notes: Abstract The aim of this study was to evaluate in vivo the cariostatic potential of the resin-reinforced glass-ionomer (Vitremer® core build-up restorative; 3M Dental Product Division) when used as a bonding agent for orthodontic brackets. The mineral distribution and topography of the enamel surface adjacent to the bracket base was determined by quantitative microradiography (TMR) and confocal laser scanning microscopy (CLSM). The study was designed in split-mouth technique using 9 pairs of premolars to be extracted for orthodontic reasons. One tooth of each pair was bonded with the resin-reinforced glass-ionomer, and the control contralateral premolar with the non-fluoridated composite (Concise®, 3M Dental Products Division). After 4 weeks all teeth were extracted and stored until analysis. The lesion depths and mineral loss values in enamel adjacent to brackets bonded with Vitremer® were significantly lower than in teeth bonded with the composite, indicating that the resin-reinforced glass-ionomers significantly reduced caries lesion development in vivo. CLSM images show a severe cariogenic challenge around orthodontic brackets and support TMR measurements. Combined TMR and CLSM provide a unique tool for examination of hard tissues exposed to caries. Fluoride releasing properties of resin-reinforced glass-ionomers represent a valuable fluoride supplement in orthodontic treatment.
    Type of Medium: Electronic Resource
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