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  • 1
    Electronic Resource
    Electronic Resource
    Effects of carbon dioxide in anther cultures (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 60 (1984), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In anther cultures of Anemone canadensis L., Anemone dichotoma L., Anemone hupehensis Lemoine, Clematis viticella L. and Papaver setigerum DC. a positive relationship between incubation in 2% CO2 and the production of microspore-derived embryos was observed. In anther cultures of Nicotiana tabacum L., Anemone hupehensis and Clematis viticella a combination of cold treatment (7°C) and incubation in 2% CO2 increased embryo production. In Anemone canadensis cold treatment increased the number of proembryos, whereas incubation in 2% CO2 had no effect. In Anemone hupehensis 5% CO2 increased embryo production by more than 2%. In Anemone dichotoma and Papaver setigerum 2% CO2 was the more efficient level. CO2 had no significant effect on pH in the culture medium in anther cultures of Anemone canadensis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1984.tb04244.x
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  • 2
    Electronic Resource
    Electronic Resource
    Uptake of 14C-L-Glutamic Acid by Daucus carota Cell Suspension in Different Shock Situations (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 38 (1976), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cell suspension cultures of Daucus carota were shocked by inoculation or by temperature, salts or osmotica in combination with inoculation. Changes in cell vitality were measured by uptake studies with labelled L-glutamic acid performed during the first 30 min after shock treatment. The cell sample was then collected on filter and counted in a liquid scintillation counter. Inoculation alone caused a marked decrease in uptake. Normal uptake capacity was re-established within 2 h. Temperatures of 0−20°C as against 27°C gave an additional decrease in uptake. Of the tested osmotica, polyethylene glycol gave the greatest stabilizing effect on the glutamic acid uptake and sucrose the least. The highest uptake rates were obtained with a calcium concentration of 0.10–0.15 mM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1976.tb04873.x
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  • 3
    Electronic Resource
    Electronic Resource
    Factors Influencing the Growth and Division of Pea Mesophyll Protoplasts (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 36 (1976), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: High yields of viable protoplasts were produced from pea leaves provided that only leaves of the same age were used in each preparation. The conditions under which the pea plants were grown and the age of the plants were also important. The protoplasts were cultured in a medium supplemented with 1 mg/1 2iP and 1 mg/1 2,4-D. They were able to regenerate cell walls within two days. After 5 days cell divisions were apparent and sustained divisions led to callus formation. Special emphasis has been given in this paper to the choice of leaf material for protoplast isolation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1976.tb03934.x
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  • 4
    Electronic Resource
    Electronic Resource
    Partial Reversal by Cytokinin and (2-Chloroethyl)-Trimethylammonium Chloride of Near-Ultraviolet Inhibited Growth and Morphogenesis in Callus Cultures (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 34 (1975), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Callus cultures of Haplopappus gracilis, Nicotiana tabacum and Allium cepa var. proliferum were in varying degrees inhibited by blue to near-UV light obtained from fluorescent tubes. The inhibition was considerably reduced for Haplopappus cultures by 6-(3-methyl-2-buten-1-ylamino)-purine (2 iP) and (2-chloro-ethyl)-trimethylammonium chloride (CCC) in combination. Even separately these compounds stimulated growth in blue but not in white light. A high concentration of 2 iP reduced the inhibiting effects of near-UV on tobacco tissue cultures, and a synergism was observed between 2 iP and CCC in respect to shoot formation in blue light. Allium callus was not significantly affected by CCC. It was also observed that the concentration of indol-3-acetic acid (IAA) was more important for the growth of tobacco cultures in blue than in white light. It is believed that the light-inhibition of growth is partly due to a photoinactivation of IAA and that 2 iP and CCC might be active through processes controlling the levels of IAA and other growth hormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1975.tb03813.x
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  • 5
    Electronic Resource
    Electronic Resource
    A Revised Medium for Growth of Pea Mesophyll Protoplasts (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 39 (1977), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The nutrient requirements of mesophyll protoplasts from Pisum sativum L. cv. Timo have been investigated and a synthetic and completely defined medium has been designed. A high calcium concentration (12 mM) stimulated both protoplast survival and cell division. The content of iron and zinc was also critical. Additions of nicotinic acid, pyridoxine and thiamine were necessary. The protoplast growth was enhanced when some amino acids were included in the medium. An absolute requirement for auxin and cytokinin was shown.In the revised medium about 90% of the isolated protoplasts survived and formed a cell wall. The first divisions were observed after 5 days and after 1 week 10–20% of the cells had divided at least once.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1977.tb01879.x
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  • 6
    Electronic Resource
    Electronic Resource
    Studies on the Growth Requirements and Growth Measurements of Cell Cultures of Haplopappus gracilis (1965)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 18 (1965), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1965.tb06994.x
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  • 7
    Electronic Resource
    Electronic Resource
    Initial stages in the course of adventitious bud formation on embryos of Picea abies (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 64 (1985), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Adventitious buds on embryos of Picea abies (L.) Karst. developed after a pulse treatment with 250 μM benzyladenine (BA) of pH 5.5 for 2 h. Light and temperature regimes were not critical during the initial stages. Adventitious buds developed faster after a pulse treatment and the variation among different experiments was lower compared to when the embryos were cultured on media supplemented with BA. Various stages of the differentiation of adventitious buds were identified: stage 1 - appearance of meristematic centres (approximately the first two weeks); stage 2 - development of adventitious bud primordia (approximately the third week); stage 3 - adventitious bud development (from approximately the 4th to the 8th week). This system may be used for further studies on bud differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1985.tb01210.x
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  • 8
    Electronic Resource
    Electronic Resource
    Induction of Adventitious Buds on Buds of Norway Spruce (Picea abies) Grown in vitro (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 45 (1979), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Resting vegetative buds of Norway spruce, Picea abies (L.) Karst., were induced to form adventitious bud primordia when cultured on medium -containing cytokinin. After transfer of the induced buds to medium lacking cytokinin, adventitious buds developed. The adventitious buds arose from meristems formed de novo in the needle primordia. No differences were found in the ability to form adventitious buds among buds collected from trees ranging from 5–50 years old.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1979.tb01658.x
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  • 9
    Electronic Resource
    Electronic Resource
    The Effect of Activated Charcoal on Tissue Cultures: Adsorption of Metabolites Inhibiting Morphogenesis (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 43 (1978), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cell suspensions of Daucus carota and Haplopappus gracilis and callus suspensions of Allum cepa var. proliferum were grown in media with and without activated charcoal. Differentiation occurred in those Daucus and Allium cultures that contained charcoal. It was shown by mass spectrometry that the media without charcoal contained high amounts of phenylacetic acid and p-OH-benzoic acid (Daucus), 2,6-OH-benzoic acid (Allium) and benzoic acid, pelargonic acid and caprylic acid (Haplopappus), whereas the media with activated charcoal did not. It was also shown that p-OH-benzoic acid had inhibitory effects on the embryogenesis in Daucus cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1978.tb01575.x
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  • 10
    Electronic Resource
    Electronic Resource
    Protease activities in non-embryogenic and embryogenic carrot cell strains during callus growth and embryo formation (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 62 (1984), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Embryogenic and non-embryogenic cell strains of Daucus carota L. were examined for their protease activity using a wide range of chromogenic synthetic peptides as substrates. High arginine-specific activity was present in all strains, but no protease activity “specific” for embryogenic or non-embryogenic strains could be detected with the substrates tested. The specific protease activity was 5–10 times higher in the non-embryogenic as compared to the embryogenic strain for most tested substrates, and this difference was not due to release of proteases in the latter. All strains showed a decrease in protease activity when cultured in media without 2,4-dichlorophenoxyacetic acid, but the embryos had high protease activity in comparison with the nondifferentiated cell aggregates. In the latter aggregates, hydrolyzing activity towards three of the substrates (H-D-Phe-Pip-Arg-p-nitroanilide, Suc-Ala-Pro-Phe-p-nitro-anilide and Bz-Phe-Val-Arg-p-nitroanilide) was absent, whereas the embryos were able to hydrolyze them.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1984.tb04603.x
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