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  • 1
    Electronic Resource
    Electronic Resource
    'Arc' escorts siRNAs in heterochromatin assembly (2007)
    [s.l.] : Nature Publishing Group
    Nature structural & molecular biology 14 (2007), S. 178-179 
    Details
    ISSN: 1545-9985
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] RNAi is the process where double-stranded RNAs induce post-transcriptional gene silencing (PTGS). The initial discovery of RNAi by Andrew Fire and Craig Mello was awarded last year's Nobel prize in Physiology and Medicine. In the RNAi mechanism, an RNase III–like enzyme, Dicer, processes ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nsmb0307-178
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  • 2
    Electronic Resource
    Electronic Resource
    RNA-interference-directed chromatin modification coupled to RNA polymerase II transcription (2005)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 435 (2005), S. 1275-1279 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] RNA interference (RNAi) acts on long double-stranded RNAs (dsRNAs) in a variety of eukaryotes to generate small interfering RNAs that target homologous messenger RNA, resulting in their destruction. This process is widely used to ‘knock-down’ the expression of genes of interest to ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature03652
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  • 3
    Electronic Resource
    Electronic Resource
    Physical mapping of the Schizosaccharomyces pombe histone genes (1994)
    Springer
    Current genetics 26 (1994), S. 553-556 
    Details
    ISSN: 1432-0983
    Keywords: S. pombe ; Histone genes ; Gene mapping ; Physical mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The histone-encoding genes in Schizosaccharomyces pombe were physically mapped by hybridisation to filters containing cosmid and P1 genomic libraries. The H2A.2 gene and the H2A.1-H2B.1 gene pair mapped between the ade6 and rikl genes on chromosome III. The three H4–H3 gene pairs were mapped to three different regions by a H4.1 probe. Southern analysis of clones from each region revealed the positions of the three H4–H3 gene paris. H4.1–H3.1 was localised to chromosome I between the mei2 and rad1 genes; H4.2–H3.2 mapped between rad3 and cdc2 on chromosome II; H4.3–H3.3 was localised to a region between the nuc1 and puc1 genes on chromosome II.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00309949
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  • 4
    Electronic Resource
    Electronic Resource
    Transient inhibition of histone deacetylase activity overcomes silencing in the mating-type region in fission yeast (1999)
    Springer
    Current genetics 35 (1999), S. 82-87 
    Details
    ISSN: 1432-0983
    Keywords: Key words Trichostatin A ; Repression ; Schizosaccharomyces pombe ; Heterochromatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have investigated the effects of inhibition of histone de-acetylase activity on silencing at the silent mating-type loci in fission yeast. Treatment of exponentially growing cells with the histone deacetylase inhibitor, trichostatin A (TSA), resulted in derepression of a marker gene inserted 150 bp distal from the silent mat3-M locus. The natural targets for the silencing mechanism in this region were only partially derepressed and the activation appeared to be asymmetric, i.e. the mat2-P cassette remained silent at concentrations that clearly partially derepressed the mat3-M cassette. We further noted that treatment of wild-type h 90 cells resulted in the generation of altered sporulation phenotypes, indicating that the treatment affected the expression of mating-type genes and/or mating-type switching. The results are discussed in the light of recent accumulated data regarding the role of deacetylation for silencing in other species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050436
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  • 5
    Electronic Resource
    Electronic Resource
    Trans-acting factors and properly positioned DNA elements repress mating-type genes in fission yeast (1992)
    Springer
    Current genetics 21 (1992), S. 331-338 
    Details
    ISSN: 1432-0983
    Keywords: Fission yeast ; Silent mating-type genes ; Trans-acting factors ; Derepressing mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Repression of the mating-type P genes at the silent mat2-P locus in fission yeast is dependent on four cis-acting DNA elements, two on each side of the coding sequences. The mechanism by which these elements exert their influence on the mating-type promoter is studied here by insertion of a bacterial antibiotic resistance gene at several positions in the silent region. The behavior of the resistance gene itself, and the changes its insertion causes in mating-type expression, reveal that the repressive elements have a limited range of action and that the four elements have unequal effects on gene expression. Repression of the antibiotic resistance gene inside the silent region leads to an antibiotic-sensitive phenotype and facilitates the selection of resistant mutants. These mutants can de-repress the resistance gene at other positions than the one used for their selection. Strong antibiotic resistance correlates with derepression of the plasmid-borne mating-type cassette. These data argue that mat2-P repression is dependent on trans-acting factors and the positioning of the repressive DNA elements, but less dependent on the nature of the affected promoter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351691
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  • 6
    Electronic Resource
    Electronic Resource
    TheNAM8 gene inSaccharomyces cerevisiae encodes a protein with putative RNA binding motifs and acts as a suppressor of mitochondrial splicing deficiencies when overexpressed (1992)
    Springer
    Molecular genetics and genomics 233 (1992), S. 136-144 
    Details
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; Nuclear gene ; Mitochondrial splicing ; Suppression ; RNA binding proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have characterized the nuclear geneNAM8 inSaccharomyces cerevisiae. It acts as a suppressor of mitochondrial splicing deficiencies when present on a multicopy plasmid. The suppressed mutations affect RNA folding and are located in both group I and group II introns. The gene is weakly transcribed in wildtype strains, its overexpression is a prerequisite for the suppressor action. Inactivation of theNAM8 gene does not affect cell viability, mitochondrial function or mitochondrial genome stability. TheNAM8 gene encodes a protein of 523 amino acids which includes two conserved (RNP) motifs common to RNA-binding proteins from widely different organisms. This homology with RNA-binding proteins, together with the intronic location of the suppressed mitochondrial mutations, suggests that the NAM8 protein could be a non-essential component of the mitochondrial splicing machinery and, when present in increased amounts, it could convert a deficient intron RNA folding pattern into a productive one.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587571
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  • 7
    Electronic Resource
    Electronic Resource
    Repression of a mating type cassette in the fission yeast by four DNA elements (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 7 (1991), S. 745-755 
    Details
    ISSN: 0749-503X
    Keywords: Fission yeast ; mating type expression ; silencer ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The fission yeast, Schizosaccharomyces pombe, expresses one of two alternative mating types. They are specified by one of two determinants (M or P) present at the mat1 locus. In addition, silent copies of M and P are present on the same chromosome. In the present work we demonstrate that the difference between the active and the silent stage of the P determinant is controlled by four repressive elements that are located at the silent locus. There are two elements to the left and two to the right of the mating type cassette. Both elements to the left and either one of the two elements to the right are required for an effective blockage of transcription. When they are combined, the four elements define a highly efficient silencer functionally similar to the HMRE and HMLE and HMLI silencers in Saccharomyces cerevisiae. In addition, the DNA surrounding the silent P locus confers symmetric partitioning in mitosis to Schizosaccharomyces pombe ars plasmids.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320070709
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