ISSN:
1572-9699
Keywords:
Escherichia coli
;
specific respiration rate
;
stoichiometric growth model
;
growth yields
;
A-stat
;
ATP
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The growth characteristics of Escherichia coli K-12 in the continuous culture with a smooth increase in the dilution rate (A-stat) of various carbon sources (glucose, acetate, succinate, glycerol, lactate, acetate + succinate, casamino, acids + glucose) were studied. For all substrates studied the maximum value of specific respiration rate, Q O2, remained between 14–18 mmol O2 h-1 g dwt-1 and the maximum growth rate varied from 0.22 h-1 on acetate to 0.77 h-1 on glucose + casamino acids. After the respiratory capacity of the cells was exhausted at growth rates µ 〈 µcrit, the growth yield YXO2, increased slightly when the dilution rate increased. The maximum growth rate of Escherichia coli K12 was dependent on growth yield, respiratory capacity and glycolytic capacity of the strain. Analysis of the cultivation data using a stoichiometric flux model indicated that ATP synthesis in E. coli exceeds by two-fold that (theoretically) required to build up biomass. The experimental value of mATP 〈 4 mmol ATP h-1 g dwt-1 determined from A-stat cultivation data was low compared with the calculated ‘unproductive hydrolysis’ of ATP (64–103 mmole ATP g dwt-1).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1000198404007
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