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Genetic analysis of the Erwinia chrysanthemi 3937 chrysobactin iron-transport system: characterization of a gene cluster involved in uptake and biosynthetic pathways (1991)

Franza, T. ; Enard, C. ; Gijsegem, F. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Molecular microbiology 5 (1991), S. 0

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ISSN: 1365-2958

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology , Medicine

Notes: Twenty of the twenty-two MudII1734 insertions impairing the chrysobactin iron-assimilation system of Erwinia chrysanthemi 3937 were localized to a 50kbp genomic insert contained in the R-prime plasmid, R′4 (Enard et al., 1988). Using the conjugative plasmid pULB1 10 (RP4::mini-Mu) and the generalized transducing phage ΦEC2, we located this iron-transport region and the two unlinked mutations on the chromosome linkage map. Chrysobactin is a catechol-type siderophore and, as we have previously observed with the entA locus of Escherichia coli, the E. chrysanthemi-derived R′4 was found to complement E. coli entB and entE mutations. A 2.9 kb Eco Ri and a 4.8kb BamHI fragment in the R′4 sharing homology with the E. coli entCEBAP15 operon DNA were subcloned. These fragments were used as DNA/DNA hybridization probes to screen a wild-type gene library, yielding a recombinant cosmid (pEC7) able to complement mutations disrupting the 2,3-drhydroxy-benzoic acid biosynthetic pathway in both Erwinia and Escherichia spp. as well as the E. coli entE mutation. Physical mapping of the genomic MudII1734 insertions corresponding to these mutations led to the identification of a cluster of genes confined to a DNA sequence of about 10 kb required for both biosynthetic and receptor functions.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb00778.x

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The requirement of chrysobactin dependent iron transport for virulence incited by Erwinia chrysanthemi on Saintpaulia ionantha (1991)

Enard, C. ; Franza, T. ; Neema, C. ; [et al.]

Springer

Plant and soil 130 (1991), S. 263-271

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ISSN: 1573-5036

Keywords: bacteria ; Erwinia chrysanthemi ; iron transport ; phytopathogenicity ; Saintpaulia ionantha ; virulence

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract To incite a systemic disease on its specific host, Saintpaulia ionantha, the soft-rot Erwinia chrysanthemi strain 3937 requires a functional high affinity iron transport system. Under iron starvation, strain 3937 produces chrysobactin, a novel catechol-type siderophore. Recent advances in the biochemistry and genetics of iron assimilation in E. chrysanthemi are reported. Analysis of leaf intercellular fluid from healthy and infected plants suggests: (i) leaf vessels in which the bacteria develop during infection would be low in free iron and (ii) chrysobactin could be produced in planta.