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  • 1
    Electronic Resource
    Electronic Resource
    CHANGES INDUCED BY PROLONGED ADMINISTRATION OF NICOTINE IN THE DISTRIBUTIONS OF CHOLINESTERASES AND ACID PHOSPHATASE IN THE ADRENAL MEDULLA OF THE RAT (1959)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 4 (1959), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1959.tb13214.x
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  • 2
    Electronic Resource
    Electronic Resource
    Separation of Substrate-specific Brain Esterases by Starch-gel Electrophoresis (1962)
    [s.l.] : Nature Publishing Group
    Nature 193 (1962), S. 778-779 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Whole brains of albino rats were homogenized with an equal amount of distilled water in a glass homo-genizer. The homogenate was frozen and thawed 6 times and centrifuged for 15 min. at an acceleration rate of 1,000〈/. The supernatant was subjected to starch electrophoresis using the technique ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/193778a0
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  • 3
    Electronic Resource
    Electronic Resource
    An efficient freeze-drying apparatus (1967)
    Springer
    Histochemistry and cell biology 8 (1967), S. 377-379 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A freeze-drying apparatus with a practical temperature regulating system is described. Degassing, drying and embedding all take place in the drying chamber. The drying temperature can be set at a number of fixed temperature levels which may be chosen anywhere between -70° C and +60° C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401980
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  • 4
    Electronic Resource
    Electronic Resource
    Specific demonstration of acetylcholinesterase and non-specific cholinesterase in the adrenal gland of the rat (1959)
    Springer
    Histochemistry and cell biology 1 (1959), S. 257-267 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Distribution of cholinesterase in the adrenal medulla of the rat was studied using acetylthiocholine, butyrylthiocholine and α-naphtyl acetate as substrates and eserine, di-isopropylfluorophosphate (DFP), 1:5-bid-(4-trimethylammoniumphenyl)-pentan-3-one di-iodide (62. C. 47) and tetra-isopropylpyrophosphoramide (iso-OMPA) as inhibitors. Acetylcholinesterase was observed in the nerve trunks, the ganglion cells, the coarse and the fine nerve fibers. The fine medullary network showed along the fibers small strongly positive ovoid bodies. Non-specific cholinesterase was detected in the capsule, the nerve trunks, the coarse nerve fibers and the fibers surrounding the noradrenaline-containing, fluorescent medullary cell islets. A weak reaction was also seen in the cytoplasm of the medullary cells. The fine medullary fibers with the ovoid bodies were essentially negative. A method was developed to demonstrate first non-specific cholinesterase and then acetylcholinesterase in the same section. The different distributions of the two cholinesterases were confirmed with this method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00736449
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  • 5
    Electronic Resource
    Electronic Resource
    Histochemical evidence of three types of esterases in the adrenal medulla of the rat (1962)
    Springer
    Histochemistry and cell biology 2 (1962), S. 383-388 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Esterases of the adrenal medulla have been studied histochemically using alpha-naphthyl acetate and butyrate as substrates, Blue RR Salt as a coupler and eserine and E, 600 as inhibitors. Three types of esterase activity were thus demonstrated: (1) cholinesterase activity in the nerve fibres, ganglion cells and secretory medullary cells; (2) eserine resistant but E. 600 sensitive esterase activity in the ganglion cells and secretory cells; (3) E. 600 resistant activity in strongly positive, unidentified cells scattered in the medulla. The histochemical picture was essentially similar in sections of formalin-fixed tissue and in fresh sections subjected to the voltage gradient employed for electrophoretic separation of esterases. It is concluded that esterases histochemically demonstrable in sections are desmo-enzymes and at least to a major part different from the lyo-enzymes which can be separated by starch gel electrophoresis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00736493
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  • 6
    Electronic Resource
    Electronic Resource
    Fine structural changes caused by 6-OH-dopamine in the small intensely fluorescent cells of the paracervical ganglion of the rat (1974)
    Springer
    Cell & tissue research 152 (1974), S. 437-447 
    Details
    ISSN: 1432-0878
    Keywords: 6-OH-dopamine ; Autonomic ganglia ; SIF cells ; Electron and Fluorescence microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The formaldehyde-induced fluorescence method was used for light microscopy and fixation in glutaraldehyde and osmium tetroxide for electron microscopy to study the effect of 75 mg/kg 6-hydroxydopamine on the paracervical ganglion of the rat uterus. No changes were observed in the fluorescence intensity of the ganglion. Ultrastructurally the ganglion cells and the synapses remained unchanged. The small, intensely fluorescent (SIF) cells were severely damaged, displaying large vacuoles with an irregular contour as well as smaller, more regular vacuoles studded with ribosomes. The granular vesicles showed a lower density of their central parts suggesting that their catecholamine content had diminished.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218930
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  • 7
    Electronic Resource
    Electronic Resource
    Fluorescence histochemical and electron microscopical observations on sympathetic ganglia of the chick embryo cultured with and without hydrocortisone (1975)
    Springer
    Cell & tissue research 156 (1975), S. 145-166 
    Details
    ISSN: 1432-0878
    Keywords: Sympathetic ganglion ; Chick embryo ; Hydrocortisone ; Culture ; Fluorescence microscopy, electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Lumbar sympathetic ganglia of 12-day-old chick embryos were cultured in organ cultures for 14 days with 1, 10 or 100 mg/l of hydrocortisone or without it. Catecholamines were demonstrated by the formaldehyde-induced fluorescence method. For electron microscopy, the cultures were fixed with glutaraldehyde and osmium tetroxide. Two types of cells with catecholamine fluorescence were observed in the control cultures: (1) weakly fluorescent sympathetic neurons and sympathicoblasts with long nerve fibres, which were the most common cell type in the explant, and (2) brightly fluorescent cells with or without fluorescent processes, which were less common and were scattered in the explant. Hydrocortisone caused a great increase in the number of the brightly fluorescent cells. With 10 mg/l of hydrocortisone the increase was about ten-fold as compared with the control cultures. There was no change in the morphology of the cells, nor could any change be observed in the fluorescence intensity by eye. Electron microscopically the mature neurons were the most common cell type on the surface of the culture, while more immature sympathicoblasts were seen in the deeper layers. Cells were also found which contained large numbers of catecholamine-storing granular vesicles 105–275 nm in diameter. These cells were infrequent. They had round vesicular nuclei and resembled also in other respects sympathicoblasts or young nerve cells. One such cell was found in mitotic division by electŕon microscopy. Hydrocortisone caused a marked increase in the number of these granule-containing cells and their processes. Cells which could have been classified as the small intensely fluorescent cells of the mammalian ganglion type or their electron microscopic equivalent, the granule-containing cells were found neither in the control cultures nor in the hydrocortisone-containing cultures. It is concluded that most brightly fluorescent cells in cultured sympathetic ganglia of the chick are nerve cells or sympathicoblasts rich in amine-storing granular vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221799
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  • 8
    Electronic Resource
    Electronic Resource
    Effect of hydrocortisone on the ultrastructure of the small, intensely fluorescent, granule-containing cells in cultures of sympathetic ganglia of newborn rats (1972)
    Springer
    Cell & tissue research 134 (1972), S. 297-310 
    Details
    ISSN: 1432-0878
    Keywords: Sympathetic Ganglia ; Tissue culture ; Catecholamines ; SIF cells ; Granular vesicles ; Hydrocortisone ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sympathetic chain ganglia of newborn rats were cultured in Rose chambers with or without hydrocortisone. After one week, the cultures were examined by light microscopy for formaldehyde-induced catecholamine fluorescence and by electron microscopy after fixation in 5% glutaraldehyde solution and thereafter in 1% osmium tetroxide. Hydrocortisone (10 mg/l) caused a great increase in the number of the small, intensely fluorescent (SIF) cells in the ganglion explants, and the fluorescence intensity of these cells was also increased. The SIF cells corresponded to small, granule-containing (SGC) cells in the electronmicros copic preparations, and in addition to an increase in their number there was also an increase in the size and number of granular vesicles in the presence of hydrocortisone. In control cultures the granular vesicles were round (about 100 nm in diameter) or elongated (40–150 nm in cross section and 150–250 nm in length); both types of vesicles contained electron dense cores. In hydrocortisone-containing cultures round granular vesicles up to 200 nm in diameter were also observed; the cores of these vesicles were of variable electron density. It is concluded that in tissue culture, hydrocortisone causes an increased formation of catecholamine-containing granular vesicles in SIF-SGC cells and their precursors and an increase in the number of these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307167
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of hydrocortisone on the extra-adrenal intensely fluorescent chromaffin and non-chromaffin cells in stretch preparations of the newborn rat (1973)
    Springer
    Cell & tissue research 146 (1973), S. 565-578 
    Details
    ISSN: 1432-0878
    Keywords: Sympathetic neurons ; Chromaffin cells ; SIP cells (Newborn rat) ; Development ; Administration of hydrocortisone ; Stretch preparations ; Fluorescence microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Air-dried stretch preparations were used to study adrenergic nerve fibres and catecholamine-containing cells with the formaldehyde-induced fluorescence method in the abdominal tissue block containing the para-aortic paraganglia, in the neurovascular structures of the cervical region including the carotid body, in the bladder, in the ileum, in the mesentery, in the vagus nerve and in the sympathetic ganglia of 5- or 15-day-old rats. The adrenergic nerves and the catecholamine-containing cells were well preserved and showed little or no diffusion of amines. While most intensely fluorescent cells of the main para-aortic body disappeared during the first two postnatal weeks, some such cells survived and they showed long, slender fluorescent processes. Administration of 20 mg/kg of hydrocortisone acetate daily for 5 days after birth caused a striking increase in the number and size of the clusters of the intensely fluorescent cells in the organ of Zuckerkandl, in the sympathetic ganglia and in the bladder, as well as an increase in the fluorescence intensity of the carotid body. In rats treated with hydrocortisone for 5 days and left to recover for 10 days an increased fluorescence was still observed. However, in the organ of Zuckerkandl the intensely fluorescent cells of hydrocortisone-treated 15-day-old rats showed less processes than those of the control rats of the same age.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02347184
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  • 10
    Electronic Resource
    Electronic Resource
    Histochemical evidence of chemical sympathectomy by guanethidine in newborn rats (1971)
    Springer
    Journal of molecular histology 3 (1971), S. 451-456 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Synopsis Guanethidine is known to cause a loss of catecholamines from sympathetically innervated tissues and sympathetic ganglia in adult animals but its effect on newborn animals has not been examined. Newborn rats were injected daily with guanethidine (20 mg/kg body weight) for 8 days. They were killed when 1 month-old along with untreated litter mate controls. Catecholamines were demonstrated in the iris, in the pineal body and in sympathetic ganglia, using the formaldehyde-induced fluorescence method. In the guanethidine-treated rats there was a complete loss of fluorescent nerve fibres from the pineal body and an almost complete loss of similar fibres from the iris. The sympathetic ganglia were reduced to less than 10% of the control ganglia, and the number of nerve cell bodies per unit area was decreased in the ganglion remnants. It is concluded that guanethidine causes, in newborn rats, an irreversible destruction of most sympathetic neurons, i.e. a chemical sympathectomy closely resembling that obtainable in newborn animals by injections of 6-hydroxydopamine or antiserum to nerve growth factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01014783
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