ISSN:
1617-4623
Keywords:
Daucus carota L.
;
Somatic segregation
;
Somatic embryogenesis
;
Somaclonal variation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract During the establishment of an embryogenic cell line from a carrot hypocotyl explant, processes closely resembling meiotic divisions are seen. A microdensitometric analysis revealed that the amount of cellular DNA diminished in the majority of cells to the haploid level. However, the diploid level was re-established in a matter of a few days. The genetic consequences of this segregation were studied by analyzing restriction fragment length polymorphisms (RFLP) and randomly amplified polymorphic DNAs (RAPD). The results showed that the great majority of embryos regenerated from segregants and that different segregants had different genetic constitutions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00290710
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