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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 7 (1984), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Rates of uptake of 14C-labelled inorganic carbon were measured for whole Chara hispida plants, detached parts of the shoot and isolated (split-chamber technique) apices, lateral branchlets and rhizoid—node complexes.The rates of inorganic carbon uptake by the rhizoid—node complex expressed per gram fresh weight whole plant were three to four orders of magnitude less than the uptake for the whole plant. Up to 70% of the carbon taken up by the rhizoid—node complex was translocated to the shoot. After 12 h exposure to 14C-labelled inorganic carbon the concentration of 14C was greater in apices than in uppermost or central internodal cells and in all lateral branchlets, regardless of whether label was supplied to the whole plant or isolated rhizoid—node complexes. Measurement of inorganic carbon uptake by detached internodal cells and detached and isolated apices and lateral branchlets showed that lateral branchlets had the greatest rates of inorganic carbon uptake. During 12 h exposure to 14C, isolated lateral branchlets translocated to the attached shoot 55% of the labelled carbon taken up; for isolated apices this value was only 13%.It is concluded that it is highly unlikely that the rhizoid of Chara hispida could acquire a significant fraction of the whole plant requirement for inorganic carbon and that apices are sink regions for photosynthate while lateral branchlets are source regions.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 147 (1992), S. 317-319 
    ISSN: 1573-5036
    Keywords: bioassay ; maize ; root pigmentation ; vesicular arbuscular mycorrhiza
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Roots of maize (Zea mays cv W64A × W182E) infected by vesicular arbuscular mycorrhizal (VAM) fungi (Glomus versiforme (Karst) Berch or a Glomus species isolated from an alfalfa soil) exhibit a bright yellow pigmentation. The percentage of pigmented roots can be quantified by a rapid visual estimate or by a grid intersect method. Both methods gave similar estimates of VAM infection to those obtained using a grid intersect count on cleared roots stained with chlorazol black E. Thus for experimental or field evaluation where speed and quantity are important, the rapid visual estimate (less than one minute for each washed root system) yields reliable results. The yellow root intersect method takes longer (5–15 minutes per root system) but gives more reproducible results. The yellow root pigmentation is light sensitive However, root systems can be reliably assayed after 1 week when stored at 5°C in the dark or after 1 year if dried.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-2932
    Keywords: Acidic mining lakes ; microcosms ; potatoes ; controlled eutrophication ; sediment-water interface ; intact sediment cores
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The water of flooded, abandoned, open-cast pits (Tagebauseen) in the Lusatia region of eastern Germany is characterised by a high acidity (Fe3+/H+), low pH (2.5-3.5) and low concentrations of dissolved phosphorus (SRP) and carbon (DIC). Investigations are investigating the use of amendments to assist in removal of acidity from Tagebauseen through controlled eutrophication. A simple system has been developed to investigate the effects of amendments on water chemistry around the sediment-water interface. Intact, little-disturbed sediment cores (diameter 5.5 cm) with overlying water (total volume 1.5 1) from one Tagebausee (Koschen: pH 3.1, Fe 15-20 mg/l) have been incubated at low temperature (6-10 °C) to investigate the effects of organic amendments (potato) on the concentrations of some major ions in the system. In particular, the dynamics of Fe, P, N and C over time in the presence or absence of organic amendment (potato) have been investigated. Addition of potatoes results in the rising of a sharp pH gradient from near the sediment surface, and an overall increase in dissolved Fe, P and C in the system. The Fe eventually precipitated as black particulates but the SRP remained in solution. Intact sediment cores provide a useful tool for studying changes in water column and sediment pore water chemistry and the potential effects of amendments on the sediments of Tagebauseen.
    Type of Medium: Electronic Resource
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