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  • 1
    Electronic Resource
    Electronic Resource
    Procollagen III peptide and fibronectin in alcohol-related chronic liver disease: Correlations with morphological features and biochemical tests
    Amsterdam : Elsevier
    Clinica Chimica Acta 179 (1989), S. 315-322 
    Details
    ISSN: 0009-8981
    Keywords: Alcohol liver disease ; Fibronectin ; Liver fibrosis ; Procollagen III peptide
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0009-8981(89)90094-6
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Relationship between males caught with low pheromone doses and larval infestation ofArgyrotaenia pulchellana (1993)
    Springer
    Entomologia experimentalis et applicata 68 (1993), S. 165-170 
    Details
    ISSN: 1570-7458
    Keywords: Argyrotaenia pulchellana ; pheromones ; threshold ; pheromone doses ; lure age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sex pheromone traps forArgyrotaenia pulchellana Haw. (Lep. Tortricidae) were loaded with two low pheromone doses (0.1 and 0.01 mg) and tested in apple and pear orchards of northern Italy's Emilia-Romagna Region to estimate the relationship between male catches and subsequent larval infestation. While the lower dose captured fewer males, it registered an enhanced correlation to larval populations and a decrease in the number of episodes of high catches associated with no infestation. In all likelihood, the pheromone traps baited at the lower dose have a smaller active space and captured only makes within the orchard. Lures with 0.01 mg of pheromone can be replaced every 6 weeks, just as the 1 mg lures now in widespread use in Italy. A threshold of 65 adults captured from the beginning of flight is proposed for the Emilia-Romagna IPM project.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02380534
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Potato leafroll virus distribution in potato meristem tips and production of virus-free plants (1988)
    Springer
    Potato research 31 (1988), S. 511-520 
    Details
    ISSN: 1871-4528
    Keywords: autoradiography ; meristem tip culture ; single-node cuttings ; in vitro tuber production ; ELISA ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Uridine-H3 was incorporated into meristem tips of both healthy and PLRV-infected potato plants, of the cultivars, Majestic and Primura. Autoradiograms of tips pretreated with actinomycin D showed that 13 of 14 and 11 of 14 respectively, were virus free in the dome and first 4 leaf primordia, 1 and 3 were infected in the 4th leaf primordium, and 1 of Primura also in its 3rd primordium. The highest plantlet yield from cultures in vitro of meristem tips that included 4 leaf primordia (7 to 9 plantlets per meristem), was obtained by growing them in sequence on 3 sequential media, each based on Murashige and Skoog basic medium complemented with various hormones. The 2nd medium, containing benzylaminopurine (0.5 mg/l) and gibberellic acid (0.5 mg/l), elicited callus and the subsequent formation of adventitious buds. Of the plantlets of the cvs Vivaks, Primura and Majestic, 88, 91 and 100 %, respectively, were PLRV-free. Propagation in vitro, by the single-node cutting technique and tuberlet production, were successful.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357889
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  • 4
    Electronic Resource
    Electronic Resource
    Correlation of potato virus S and virus M contents of potato meristem tips with the percentage of virus-free plantlets produced in vitro (1996)
    Springer
    Potato research 39 (1996), S. 129-140 
    Details
    ISSN: 1871-4528
    Keywords: Dot-ELISA ; in vitro culture ; ribavirin ; DHT ; Solanum tuberosum L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Using sensitive Dot-ELISA, potato virus S (PVS) and potato virus M (PVM) contents per single potato meristem tip of 4-leaf primordia were determined, and were correlated with the percentage of virus-free plantlets obtained by meristem-tip culture preceded by chemo-and thermotherapy of the plants. Treatment of PVS-infected cv. Majestic plants for 26 days at 35°C resulted in 85% of the meristems having less than 10pg of virus, and 89% of the derived progenies were similarly virus-free. When stem cuttings were dipped for 15 days in various chemicals, the mixture of 5 mg/l ribavirin+5mg/l DHT was the most effective. For cvs Majestic and Primura, 90 and 72% of the meristems had less than 10pg of virus, and resulted in 74 and 67% virus-free progenies respectively. In PVM infection of the two cultivars, the same mixture yielded 70 and 66% meristems with less than 10pg of virus, and 69 and 68% virus-free progenies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358213
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  • 5
    Electronic Resource
    Electronic Resource
    Use of the polymerase chain reaction to clone the potato leafroll virus coat protein gene directly from the total RNA of infected plants (1995)
    Springer
    Potato research 38 (1995), S. 211-218 
    Details
    ISSN: 1871-4528
    Keywords: reverse transcription ; PCR. DNA sequencing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The potato leafroll virus (PLRV) coat protein (CP) gene was directly cloned from the total RNA extracted from virus-infected plants. First strand cDNA synthesis was not necessarily specific; it was equally efficient using either random or CP-specific primers. The viral sequence encoding the coat protein was specifically amplified from the total population of cDNA molecules by polymerase chain reaction (PCR), using specific primers bordering the CP gene. The unique amplified product thus obtained was cloned blunt-end into the pT7T318U plasmid vector, and the authenticity of the cloned gene verified by sequence analysis. This cloning strategy obviates the need for virus purification. Sequence comparison of the CP gene of the Italian isolate and those of five other PLRV isolates revealed a close similarity to the three European and the Canadian isolates, and a more distant relationship with the Australian one.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357934
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    Paper (German National Licenses)
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