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  • 1
    Electronic Resource
    Electronic Resource
    The role of genetic balance in control of transcription rate in the X-chromosome of Drosophila melanogaster (1974)
    Springer
    Chromosoma 45 (1974), S. 193-201 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The intensity of transcription in the polytene X-chromosome of the salivary glands of Drosophila melanogaster has been determined as a function of the ratio of X-chromosomes to autosomes (X:A). Autoradiographic measurements revealed that the single X-chromosome in males (1X2A) incorporates twice as much label as each of the two X-chromosomes in females (2X2A) which confirms the earlier report of Mukherjee and Beermann (1965). The addition of the third autosome set in super-males (1X3A) does not result in an increase of RNA synthesis in the X-chromosomes as compared to normal males (1X2A). In superfemales (3X2A) carrying an extra X-chromosome the transcription in each of the X-chromosomes proceeds with the same rate as in diploid (2X2A) or triploid (3X3A) females. In intersexes (2X3A) the rate of transcription in each of X-chromosomes is intermediate between male and female levels. — The comparison of transcription rates in X-chromosomes with the levels of two enzymes coded for by sex-linked genes in males, females, super-females and intersexes (Faizullin and Gvozdev, 1972, 1973) revealed that the dosage compensation of the enzyme synthesis is effected at the level of transcription.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00362311
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  • 2
    Electronic Resource
    Electronic Resource
    Diagnosis of urogenital chlamydiasis by dot hybridization with biotin-labeled DNA probe (1996)
    Springer
    Bulletin of experimental biology and medicine 122 (1996), S. 939-942 
    Details
    ISSN: 1573-8221
    Keywords: biotin-labeled DNA probe ; dot hybridization ; C. trachomatis ; diagnosis of chlamydiasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The DNA fragment ofC. trachomatis cryptic plasmid (1115 bp long) is cloned into pBR322 vector. The recombinant plasmid pCTp1 binds to purified chlamydial DNA and DNA isolated from epithelial cells of patients with clinical manifestations of chlamydiasis and does did not bind to DNA ofM. hominis, U. urealyticum, E. coli, herpes simplex virus, and human DNA. The probe sensitivity is 64.5%, its specificity is 84.9%, and the specificities for positive and negative results are 79.2 and 85.7%, respectively, compared with the polymerase chain reaction. These parameters are 61,6, 81.4, 78.2, and 85.6%, respectively, compared with the immunofluorescent method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02446588
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  • 3
    Electronic Resource
    Electronic Resource
    Dosage compensation of sex-linked genes in Drosophila melanogaster (1973)
    Springer
    Molecular genetics and genomics 126 (1973), S. 233-245 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The phenomenon of dosage compensation in Drosophila melanogaster which consists in doubling of the activity of the X-chromosome genes in males as compared to those in females was studied. The specific activities of 6-phosphogluconate dehydrogenase (6PGD) and glucose-6-phosphate dehydrogenase (G6PD) determined by the sex-linked structural genes Pgd and Zw respectively were studied in flies carrying duplications for different regions of the X-chromosome. The increase in dose of Pgd and Zw in females resulting from the addition of an extra X-chromosome or X-fragments leads to a proportional rise in the specific activities of 6PGD and G6PD. On the other had the addition to females of the X-chromosome carrying no Pgd gene or X-fragments lacking Pgd and Zw has no effect on the enzyme activities. Thus we failed to reveal in the X-chromosome any compensatory genes envisaged by Muller, which would repress sex-linked structural genes proportional to their dose. The 6PGD and G6PD levels in phenotypically male-like intersexes carrying two X-chromosomes and three autosome sets (2X3A) is 30% higher than in diploid (2X2A) or triploid (3X3A) females. However the specific activities of the enzymes in female-like intersexes are the same as in regular females. The levels of 6PGD and G6PD per one X-chromosome are 1.5–2.0 times higher in the intersexes than in the normal females and metafemales (3X2A). The results indicate that the level of expression of the X-chromosome is determined by the X:A ratio. It is suggested that the decreased X:A ratio in males is responsible for the hyperactivation of their X-chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267534
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  • 4
    Electronic Resource
    Electronic Resource
    Detection ofChlamydia trachomatis, Mycoplasma hominis, andUreaplasma urealyticum by polymerase chain reaction in men and women with genital diseases (1996)
    Springer
    Bulletin of experimental biology and medicine 121 (1996), S. 494-497 
    Details
    ISSN: 1573-8221
    Keywords: polymerase chain reaction ; C. trachomatis ; M. hominis ; U. urealyticum ; mixed urogenital infections
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Polymerase chain reaction was clinically used to diagnose chlamydial and mycoplasma infection in the cervix and male urethra. Examinations of 2260 patients with disorders of reproductive function detectedC. trachomatis, M. hominis, andU. urealyticum in 32.9, 9.7, and 25.7% of cases, respectively. A high incidence of mixed nongonococcal urogenital infections was observed, particularly in women. Seasonal fluctuations were shown in the detection rates of chlamydial and ureaplasmic infections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02446948
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  • 5
    Electronic Resource
    Electronic Resource
    Identification ofMycoplasma hominis, Ureaplasma urealyticum, andChlamydia trachomatis by the polymerase chain reaction (1995)
    Springer
    Bulletin of experimental biology and medicine 120 (1995), S. 1221-1224 
    Details
    ISSN: 1573-8221
    Keywords: polymerase chain reaction ; Mycoplasma hominis ; Ureaplasma urealyticum ; Chlamydia trachomatis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A procedure for identifying chlamydia, mycoplasmas, and ureaplasmas by the polymerase chain reaction is described which uses a system of four primers complementary to fragments of the 16S RNA gene, one of which detects all three microorganisms and the other three are each specific for a particular organism. With this primer system, chlamydia, mycoplasmas, and ureaplasmas are detectable in a single reaction. The procedure may be used for routine diagnoses in diagnostic laboratories.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02445577
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  • 6
    Electronic Resource
    Electronic Resource
    Potential activity of Ca2+/Mg2+-dependent endonuclease in endometrial hyperplasia and cancer (1999)
    Springer
    Bulletin of experimental biology and medicine 127 (1999), S. 624-627 
    Details
    ISSN: 1573-8221
    Keywords: apoptosis ; Ca2+/Mg2+-dependent endonuclease ; endometrial hyperplasia ; endometrial cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Potential activity of Ca2+/Mg2+-dependent endonuclease in hyperplastic endometrial tissues is lower than in normal endometrium and practically absent in endometrial cancer tissue. Thus, molecular mechanisms of apoptosis regulation are disturbed even at the stage of hyperplasia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02433297
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